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pMD2.G
  • Sequence Map
    Editing disabled on read-only sequence.
    ggatcccctgagggggcccccatgggctagaggatccggcctcggcctctgcataaataaaaaaaattagtcagccatgagcttggcccattgcatacgttgtatccatatcataatatgtacatttatattggctcatgtccaacattaccgccatgttgacattgattattgacctaggggactcccccgggggtacccgatctcctaggccggagccggagacgtatttattttttttaatcagtcggtactcgaaccgggtaacgtatgcaacataggtatagtattatacatgtaaatataaccgagtacaggttgtaatggcggtacaactgtaactaataactApaIBsrGIBstXIBsu36IHincIIPspOMINmeAIIISV40pro...primerCMV_...ter20406080100120140160
    ctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattggatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaacAseISpeICMV_immearly_promoterCAG_enhancer180200220240260280300320340
    acgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtctgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagBsaAIBtgZISnaBICMV_immearly_promoterCAG_enhancer360380400420440460480500520
    atcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttCspCICMV_immearly_promoter540560580600620640660680700
    tgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctcccctcgaagcttacatgtggtaccgagctcggatcctgagaacttcaacccgccatccgcacatgccaccctccagatatattcgtctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggggagcttcgaatgtacaccatggctcgagcctaggactcttgaagtKpnINspIPciISacISacIBsmBIEsp3IAcc65IAflIIIEco53kIEco53kIHindIIICMV2_promoterCMV_promoterCMV_immearly_promoterLNCX_primerpCEP_fwd_primerCMV_...mer720740760780800820840860
    gggtgagtctatgggacccttgatgttttctttccccttcttttctatggttaagttcatgtcataggaaggggagaagtaacagggtacacatattgaccaaatcagggtaattttgcatttgtaattttaaaaaatgctttcttcttttaatatacttttttgtttatcttatcccactcagataccctgggaactacaaaagaaaggggaagaaaagataccaattcaagtacagtatccttcccctcttcattgtcccatgtgtataactggtttagtcccattaaaacgtaaacattaaaattttttacgaaagaagaaaattatatgaaaaaacaaatagaataKflIPpuMIbGlob_int8809009209409609801,0001,0201,040
    ttctaatactttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctctttgcaccattctaaagaataacagtgataatttctgggttaaggcaatagcaatatttctgcatataaatatttctgcatataaattgtaactgatgtaagaggtttcatattaagattatgaaagggattagagaaagaaagtcccgttattactatgttacatagtacggagaaacgtggtaagatttcttattgtcactattaaagacccaattccgttatcgttataaagacgtatatttataaagacgtatatttaacattgactacattctccaaagtataabGlob_int1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gctaatagcagctacaatccagctaccattctgcttttattttatggttgggataaggctggattattctgagtccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctgggcaacgtgctggtctgtgtgctggcccatcactttggcaacgattatcgtcgatgttaggtcgatggtaagacgaaaataaaataccaaccctattccgacctaataagactcaggttcgatccgggaaaacgattagtacaagtatggagaatagaaggagggtgtcgaggacccgttgcacgaccagacacacgaccgggtagtgaaaccgttAlwNIBstXIbGlob_int1,2401,2601,2801,3001,3201,3401,3601,3801,400
    agcacgtgagatctgaattcaacagagatcgatctgtttccttgacactatgaagtgccttttgtacttagcctttttattcattggggtgaattgcaagttcaccatagtttttccacacaaccaaaaaggaaactggaaaaatgttccttctaattaccattattgcccgtcatcgtgcactctagacttaagttgtctctagctagacaaaggaactgtgatacttcacggaaaacatgaatcggaaaaataagtaaccccacttaacgttcaagtggtatcaaaaaggtgtgttggtttttcctttgacctttttacaaggaagattaatggtaataacgggcagtApoIClaIPmlIBglIIBsaAIBspDIEcoRIvsv-G1,4201,4401,4601,4801,5001,5201,5401,560
    agctcagatttaaattggcataatgacttaataggcacagccatacaagtcaaaatgcccaagagtcacaaggctattcaagcagacggttggatgtgtcatgcttccaaatgggtcactacttgtgatttccgctggtatggaccgaagtatataacacagtccatccgatccttcgagtctaaatttaaccgtattactgaattatccgtgtcggtatgttcagttttacgggttctcagtgttccgataagttcgtctgccaacctacacagtacgaaggtttacccagtgatgaacactaaaggcgaccatacctggcttcatatattgtgtcaggtaggctaggaSwaIvsv-G1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcactccatctgtagaacaatgcaaggaaagcattgaacaaacgaaacaaggaacttggctgaatccaggcttccctcctcaaagttgtggatatgcaactgtgacggatgccgaagcagtgattgtccaggtgactcctcaccatgtgctggttgatgaatacacaggagaatgagtgaggtagacatcttgttacgttcctttcgtaacttgtttgctttgttccttgaaccgacttaggtccgaagggaggagtttcaacacctatacgttgacactgcctacggcttcgtcactaacaggtccactgaggagtggtacacgaccaactacttatgtgtcctcttacPflMIDraIIIvsv-G1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggttgattcacagttcatcaacggaaaatgcagcaattacatatgccccactgtccataactctacaacctggcattctgactataaggtcaaagggctatgtgattctaacctcatttccatggacatcaccttcttctcagaggacggagagctatcatccctgggaaaggagccaactaagtgtcaagtagttgccttttacgtcgttaatgtatacggggtgacaggtattgagatgttggaccgtaagactgatattccagtttcccgatacactaagattggagtaaaggtacctgtagtggaagaagagtctcctgcctctcgatagtagggaccctttcctcBsmIPasIBsaXIEcoNIvsv-G1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggcacagggttcagaagtaactactttgcttatgaaactggaggcaaggcctgcaaaatgcaatactgcaagcattggggagtcagactcccatcaggtgtctggttcgagatggctgataaggatctctttgctgcagccagattccctgaatgcccagaagggtcaagtatctccgtgtcccaagtcttcattgatgaaacgaatactttgacctccgttccggacgttttacgttatgacgttcgtaacccctcagtctgagggtagtccacagaccaagctctaccgactattcctagagaaacgacgtcggtctaagggacttacgggtcttcccagttcatagaBplIBplIBsmIPstIStuIXcmIBsaBIBstAPIvsv-G2,1202,1402,1602,1802,2002,2202,2402,260
    ctgctccatctcagacctcagtggatgtaagtctaattcaggacgttgagaggatcttggattattccctctgccaagaaacctggagcaaaatcagagcgggtcttccaatctctccagtggatctcagctatcttgctcctaaaaacccaggaaccggtcctgctttcaccatgacgaggtagagtctggagtcacctacattcagattaagtcctgcaactctcctagaacctaataagggagacggttctttggacctcgttttagtctcgcccagaaggttagagaggtcacctagagtcgatagaacgaggatttttgggtccttggccaggacgaaagtggtaAgeIBsrFIvsv-G2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aatcaatggtaccctaaaatactttgagaccagatacatcagagtcgatattgctgctccaatcctctcaagaatggtcggaatgatcagtggaactaccacagaaagggaactgtgggatgactgggcaccatatgaagacgtggaaattggacccaatggagttctgaggaccttagttaccatgggattttatgaaactctggtctatgtagtctcagctataacgacgaggttaggagagttcttaccagccttactagtcaccttgatggtgtctttcccttgacaccctactgacccgtggtatacttctgcacctttaacctgggttacctcaagactcctggBcgIBclIBsaIKpnIBmgBIAcc65Ivsv-G2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agttcaggatataagtttcctttatacatgattggacatggtatgttggactccgatcttcatcttagctcaaaggctcaggtgttcgaacatcctcacattcaagacgctgcttcgcaacttcctgatgatgagagtttattttttggtgatactgggctatccaaaaatccaatcaagtcctatattcaaaggaaatatgtactaacctgtaccatacaacctgaggctagaagtagaatcgagtttccgagtccacaagcttgtaggagtgtaagttctgcgacgaagcgttgaaggactactactctcaaataaaaaaccactatgacccgataggtttttaggttBsaBIBstBIBpu10Ivsv-G2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgagcttgtagaaggttggttcagtagttggaaaagctctattgcctcttttttctttatcatagggttaatcattggactattcttggttctccgagttggtatccatctttgcattaaattaaagcacaccaagaaaagacagatttatacagacatagagatgaaccgactagctcgaacatcttccaaccaagtcatcaaccttttcgagataacggagaaaaaagaaatagtatcccaattagtaacctgataagaaccaagaggctcaaccataggtagaaacgtaatttaatttcgtgtggttcttttctgtctaaatatgtctgtatctctacttggctgavsv-GVSVG Tag2,8202,8402,8602,8802,9002,9202,9402,960
    tggaaagtaactcaaatcctgcacaacagattcttcatgtttggaccaaatcaacttgtgataccatgctcaaagaggcctcaattatatttgagtttttaatttttatggaattcaccccaccagtgcaggctgcctatcagaaagtggtggctggtgtggctaatgccctggcacctttcattgagtttaggacgtgttgtctaagaagtacaaacctggtttagttgaacactatggtacgagtttctccggagttaatataaactcaaaaattaaaaataccttaagtggggtggtcacgtccgacggatagtctttcaccaccgaccacaccgattacgggaccgApoIBsgIBsgIStuIEcoRI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccacaagtttcactaagctcgcttccttgctgtccaatttctattaaaggttccttggttccctaagtccaactactaaactgggggatattatgaagggccttgagcatctggattctgcctaataaaaaacatttattttcattgcaatgatgtatttaaattatttctgaatggtgttcaaagtgattcgagcgaaggaacgacaggttaaagataatttccaaggaaccaagggattcaggttgatgatttgaccccctataatacttcccggaactcgtagacctaagacggattattttttgtaaataaaagtaacgttactacataaatttaataaagacttaSwaI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    attttactaaaaagggaatgtgggaggtcagtgcatttaaaacataaagaaatgaagagctagttcaaaccttgggaaaatacactatatcttaaactccatgaaagaaggtgaggctgcaaacagctaatgcacattggcaacagccctgatgcctatgccttattcatccctctaaaatgatttttcccttacaccctccagtcacgtaaattttgtatttctttacttctcgatcaagtttggaacccttttatgtgatatagaatttgaggtactttcttccactccgacgtttgtcgattacgtgtaaccgttgtcgggactacggatacggaataagtagggagEarISapIBspQI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    agaaaaggattcaagtagaggcttgatttggaggttaaagtttggctatgctgtattttacattacttattgttttagctgtcctcatgaatgtcttttcactacccatttgcttatcctgcatctctcagccttgactccactcagttctcttgcttagagataccacctttcctcttttcctaagttcatctccgaactaaacctccaatttcaaaccgatacgacataaaatgtaatgaataacaaaatcgacaggagtacttacagaaaagtgatgggtaaacgaataggacgtagagagtcggaactgaggtgagtcaagagaacgaatctctatggtggaaagg3,5203,5403,5603,5803,6003,6203,6403,660
    cctgaagtgttccttccatgttttacggcgagatggtttctcctcgcctggccactcagccttagttgtctctgttgtcttatagaggtctacttgaagaaggaaaaacagggggcatggtttgactgtcctgtgagcccttcttccctgcctcccccactcacagtgacccggaggacttcacaaggaaggtacaaaatgccgctctaccaaagaggagcggaccggtgagtcggaatcaacagagacaacagaatatctccagatgaacttcttcctttttgtcccccgtaccaaactgacaggacactcgggaagaagggacggagggggtgagtgtcactgggcctAccIMscIBceAI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    atccctcgacatggcagtctagcactagtgcggccgcagatctgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggctagggagctgtaccgtcagatcgtgatcacgccggcgtctagacgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccgEagINotINspIPciISpeIBglIIAflIII3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttcgtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaagBssSIBssSαIpBR322_origin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcApaLIBseYIHaeIIpBR322_origin4,2204,2404,2604,2804,3004,3204,3404,360
    tcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggAlwNIBceAIpBR322_origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggatttaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaapBR322_origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaAmpicillin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataaAhdIAseIBsaIBsrFINmeAIIIAmpicillin4,9204,9404,9604,9805,0005,0205,0405,060
    aattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccAclIFspIAmpicillin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcBcgIPvuIScaIAmpicillin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaAclIXmnIApaLIBssSIBssSαIAmpicillin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatEarIAmpicillinAmpR_promoter5,6205,6405,6605,6805,7005,7205,7405,760
    aacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcaBmgBI5,7805,8005,820
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    5824 bp42504500475050005250550025050075010001250vsv-GCMV2_promoterCMV_promoterLNCX_primerSV40pro_F_primer,Bsu36I,PspOMI,ApaI,NmeAIII,BstXI,BsrGI,HincII,SpeI,AseICMV_immearly_promoter,CAG_enhancer,BtgZIBsaAI,SnaBI,CspCICMV_fwd_primer,pCEP_fwd_primer,Eco53kI,SacI,BsmBI,Esp3IHindIIIAflIIIPciINspI,Acc65I,KpnI,Eco53kI,SacI,KflI,PpuMIbGlob_intAlwNIBstXIBsaAI,PmlI,BglII,ApoI,EcoRI,BspDI,ClaIpBR322_originAlwNIBceAIAhdIBsaI,BsrFINmeAIII,AseI,FspI,AclIAmpicillin,PvuIScaI,BcgI,AclI,XmnIAmpR_promoter,BssSI,BssSαI,ApaLI,EarI,BmgBI

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    5824