Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pLenti6.3/V5-DIO-HA-hM4D(Gi)-mCherry
  • Sequence Map
    Editing disabled on read-only sequence.
    aatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactttacatcagaatacgttatgagaacatcagaacgttgtaccattgctactcaatcgttgtacggaatgttcctctctttttcgtggcacgtacggctaaccaccttcattccaccatgctagcacggaataatccttccgttgtctgcccagactgtacctaacctgcttggtgaBaeIRSV promoterpRSV/5'LTR20406080100120140160
    gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtcttaacggcgtaacgtctctataacataaattcacggatcgagctatgtatttgcccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacapRSV/5'LTRHIV-1 5' LTRRSV promoter180200220240260280300320340
    gtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtcactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcapRSV/5'LTRHIV-1 5' LTR360380400420440460480500520
    acgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgtgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcNruIHIV-1 psi packaging signal540560580600620640660680700
    cagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgtt720740760780800820840860
    gatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcactatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtMfeINotISapIBspQIEcoNI8809009209409609801,0001,0201,040
    gagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacBbvCIRRE1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaaaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaccttBsmIBsaBIRRE1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatat1,4201,4401,4601,4801,5001,5201,5401,560
    aaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagtttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctcKflIPpuMI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acagagacagatccattcgattagtgaacggatctcgacggtatcggttaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatgtctctgtctaggtaagctaatcacttgcctagagctgccatagccaattgaaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaatagctClaIHpaIBspDIcPPT1,7601,7801,8001,8201,8401,8601,8801,9001,920
    taagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagattcgaaccctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcPcmv1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatggatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttaccNdeISnaBIPcmv2,1202,1402,1602,1802,2002,2202,2402,260
    gcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgaPcmv2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgactctagaggatccactagtggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggctgagatctcctaggtgatcacctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcaatacgtcttaccatcgacctaacatcgacgatSalISpeIBsmBIBspEIEsp3IPcmvLox27222,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccttacttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgaatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcggaatgaacatgtcgagcaggtacggcggccacctcaccgccgggagccgcgcaagcatgacaaggtgctaccacatcaggagcaacaccctccactacaggttgaactacaacAscIXcmISgrAImCherryLoxP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    acgttgtaggcgccgggcagctgcacgggcttcttggccttgtaggtggtcttgacctcagcgtcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttcttgcaacatccgcggcccgtcgacgtgcccgaagaaccggaacatccaccagaactggagtcgcagcatcaccggcggcaggaagtcgaagtcggagacgaactagagcgggaagtcccgcggcaggagccccatgtaggcgagcctcctccggagggtcgggtaccagaagaagaBbvCIPflMImCherry2,8202,8402,8602,8802,9002,9202,9402,960
    gcattacggggccgtcggaggggaagttggtgccgcgcagcttcaccttgtagatgaactcgccgtcctgcagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcggggatcgtaatgccccggcagcctccccttcaaccacggcgcgtcgaagtggaacatctacttgagcggcaggacgtccctcctcaggacccagtgccagtggtgcggcggcaggagcttcaagtagtgcgcgagggtgaacttcgggagccccttcctgtcgaagttcatcagcccctaAloISbfIBstEIImCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtcggcggggtgcttcacgtaggccttggagccgtacatgaactgaggggacaggatgtcccaggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagcagccgccccacgaagtgcatccggaacctcggcatgtacttgactcccctgtcctacagggtccgcttcccgtcccccggtgggaaccagtggaagtcgaaccgccagacccacgggagcatccccgccgggagcgggagcgggagctagagcttgagcaccggcaagtgcctcAhdIBstEIImCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccctccatgtgcaccttgaagcgcatgaactccttgatgatggccatgttatcctcctcgcccttgctcaccatggtggcgaccggtggatcccgcctggcagtgccgatgttccgatactggcacagcagcaggtgccggaaggtctttttaaaggtggcgttgcacagagcatgggaggtacacgtggaacttcgcgtacttgaggaactactaccggtacaataggaggagcgggaacgagtggtaccaccgctggccacctagggcggaccgtcacggctacaaggctatgaccgtgtcgtcgtccacggccttccagaaaaatttccaccgcaacgtgtctcgtaBfuAIBspMIPaqCIhM4DmCherry3,3403,3603,3803,4003,4203,4403,4603,4803,500
    agcaggcagggttgatggtgctgttgacgtagcagagccagtagccaatggaccacaccgtgtcagggatgcagctctggcagaaggtgttcaccaggaccatgacgttgtagggcgtccaggtgaggatgaaggccagcagaatggcaaagatcgttcgtgtcactttgcgctctcgtccgtcccaactaccacgacaactgcatcgtctcggtcatcggttacctggtgtggcacagtccctacgtcgagaccgtcttccacaagtggtcctggtactgcaacatcccgcaggtccactcctacttccggtcgtcttaccgtttctagcaagcacagtgaaacgcgagDraIIIhM4D3,5203,5403,5603,5803,6003,6203,6403,660
    ccgggccgccatctgccgcttcttgcgcacctggttgcgagcgatgctggcgaacttgcgggccacgttggccgcagggcgcatgccagccggcgtggcaggcacaatctcaatggctgtcacacactcattgcctgtctgcttcgtcacaatctggatcttggaccatctggagggcccggcggtagacggcgaagaacgcgtggaccaacgctcgctacgaccgcttgaacgcccggtgcaaccggcgtcccgcgtacggtcggccgcaccgtccgtgttagagttaccgacagtgtgtgagtaacggacagacgaagcagtgttagacctagaacctggtagacctcSfiIXcmISexAIhM4D3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gctgggttgagggcccgcggctgcaggggaggggcgggcatggcgggcgtggtggcctctgtggtggacagctctgtggctgggcgttccttggtgttctgggtggcactgcctgagctggactcattggaagtgtccttatcagccacggggcgcggtggcggtggcagcgctgcgacccaactcccgggcgccgacgtcccctccccgcccgtaccgcccgcaccaccggagacaccacctgtcgagacaccgacccgcaaggaaccacaagacccaccgtgacggactcgacctgagtaaccttcacaggaatagtcggtgccccgcgccaccgccaccgtcgcgacAfeIhM4D3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcgggggggcctcctccagcttgccattgcgcagctcctcccgggcggcctccccgggcgggggcttcttgacgctctgcttcattagtgggctcttgaggaaggccagcgtcttggctttcttctccttcgggccctcgggccggtgcttgtggactcggctgcgactggccagcgcccccccggaggaggtcgaacggtaacgcgtcgaggagggcccgccggaggggcccgcccccgaagaactgcgagacgaagtaatcacccgagaactccttccggtcgcagaaccgaaagaagaggaagcccgggagcccggccacgaacacctgagccgacgctgaccggtchM4D4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggagatgtggatgtacagcaccgtcatgatgaccacaggcaggtagaagccagcaatggctgtgccaaaggtcactgctgggttggacaggaactggatgaagcactggttgtcgggcaccgtccgcttacccaccacaaactgccagaacaagatggcaggcgcccagagcacgcctctacacctacatgtcgtggcagtactactggtgtccgtccatcttcggtcgttaccgacacggtttccagtgacgacccaacctgtccttgacctacttcgtgaccaacagcccgtggcaggcgaatgggtggtgtttgacggtcttgttctaccgtccgcgggtctcgtgcBfuAIBspMIhM4D4,2204,2404,2604,2804,3004,3204,3404,360
    aaggacagtacccaggcagcagcaatcatgaggcctgccatcttggtggtgcgccgggcagggtaggtgagaggcttggtgacgcagaagtagcggtcaaagctgatgatgagaaggttcatgacggaggcgttgctcaccacgcagtccagggccagccacaggtcgcagaccattcctgtcatgggtccgtcgtcgttagtactccggacggtagaaccaccacgcggcccgtcccatccactctccgaaccactgcgtcttcatcgccagtttcgactactactcttccaagtactgcctccgcaacgagtggtgcgtcaggtcccggtcggtgtccagcgtctggthM4D4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cggcgcccaggggccagtagcccttgatgatgtacacggtgtagaggttcatggagaaggcgcctatgatgagatcagcacacgccaggctgaagaggaagtagttgttgactgtctgcagctgcctgttgaccttgatggacagcatcaccaggatgttgcccacgacagtcacgccgcgggtccccggtcatcgggaactactacatgtgccacatctccaagtacctcttccgcggatactactctagtcgtgtgcggtccgacttctccttcatcaacaactgacagacgtcgacggacaactggaactacctgtcgtagtggtcctacaacgggtgctgtcagtgPasIhM4D4,5604,5804,6004,6204,6404,6604,6804,7004,720
    caggctcagggagcctgtcactgtggcaatgaagaccatttccaccgtctcatagcgattgtgggatgatgacgtgaccaggcgcacggactgattgcccgagctgccattgacaggtgtgaagttggccatggtggcgctagcataacttcgtataaagtatcctatacgaagtgtccgagtccctcggacagtgacaccgttacttctggtaaaggtggcagagtatcgctaacaccctactactgcactggtccgcgtgcctgactaacgggctcgacggtaactgtccacacttcaaccggtaccaccgcgatcgtattgaagcatatttcataggatatgcttcaBmtINheIBmgBIBsmBIEsp3IhM4DLox27224,7404,7604,7804,8004,8204,8404,8604,8804,900
    tatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgactagtggatccgagctcggtaccaagcttaagtttaaaccgctgatcagcctcgactgtgccttcataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcgaatagctgatcacctaggctcgagccatggttcgaattcaaatttggcgactagtcggagctgacacggaagBclIPmeISpeIEcoRIEcoRVLoxP4,9204,9404,9604,9805,0005,0205,0405,060
    taaagggccaattctgcagatatccagcacagtggcggccgctcgagtctagagggcccgcggttcgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggttagtaatgatcgacaatcaacctctggattacaaaatttgtgaaagattgactggatttcccggttaagacgtctataggtcgtgtcaccgccggcgagctcagatctcccgggcgccaagcttccattcggatagggattgggagaggagccagagctaagatgcgcatggccaatcattactagctgttagttggagacctaatgttttaaacactttctaactgaccMluINotITliIXhoIBstBIEcoNIEcoRVPspXIPaeR7IWPREV5 epitope5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgaWPRE5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggcacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccPfoIPflMIWPRE5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctaWPRE5,6205,6405,6605,6805,7005,7205,7405,760
    ctccctttgggccgcctccccgcctggcgatggtaccggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcaggagggaaacccggcggaggggcggaccgctaccatggccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcBaeINsiINsiISexAISV40 promoterWPRE5,7805,8005,8205,8405,8605,8805,9005,9205,940
    caaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttggttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacAvrIISV40 promoter5,9605,9806,0006,0206,0406,0606,0806,1006,120
    caaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccaagcctttgtctcaagaagaatccaccctcattgaaagagcaacggctacaagtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacaactgttaattagtagccgtatcatatagccgtatcatattatgctgttccactccttgatttggtaccggttcggaaacagagttcttcttaggtgggagtaactttctcgttgccgatgttBclIPmlIEM7Blasticidin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tcaacagcatccccatctctgaagactacagcgtcgccagcgcagctctctctagcgacggccgcatcttcactggtgtcaatgtatatcattttactgggggaccttgtgcagaactcgtggtgctgggcactgctgctgctgcggcagctggcaacctgacttgtatcgtcgcagttgtcgtaggggtagagacttctgatgtcgcagcggtcgcgtcgagagagatcgctgccggcgtagaagtgaccacagttacatatagtaaaatgaccccctggaacacgtcttgagcaccacgacccgtgacgacgacgacgccgtcgaccgttggactgaacatagcagcgNruIPpuMIBlasticidin6,3206,3406,3606,3806,4006,4206,4406,460
    gatcggaaatgagaacaggggcatcttgagcccctgcggacggtgccgacaggtgcttctcgatctgcatcctgggatcaaagccatagtgaaggacagtgatggacagccgacggcagttgggattcgtgaattgctgccctctggttatgtgtgggagggctaagcacaattcctagcctttactcttgtccccgtagaactcggggacgcctgccacggctgtccacgaagagctagacgtaggaccctagtttcggtatcacttcctgtcactacctgtcggctgccgtcaaccctaagcacttaacgacgggagaccaatacacaccctcccgattcgtgttaagBlpIBsaBIBlasticidin6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gagctcggtacctttaagaccaatgacttacaaggcagctgtagatcttaaccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggcctcgagccatggaaattctggttactgaatgttccgtcgacatctagaattggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggttgcttctgttctagacgaaaaacgaacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgdeltaU3/3'LTRdelta U33' LTR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    taactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtcataaatattgdeltaU3/3'LTR3' LTR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcaacgtttctttacttatagtctctcactctccttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccgagatcgatagBsmISV40 pA7,0207,0407,0607,0807,1007,1207,1407,160
    ccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagggacgtacccaattcgccggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccctgcatgggttaagcggSfiIAvrII7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatgatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggactta7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcccttccgcttaccctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaa7,5407,5607,5807,6007,6207,6407,6607,6807,700
    tagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagAloIDraIII7,7207,7407,7607,7807,8007,8207,8407,860
    ggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgSspIbla (amp) promoter7,8807,9007,9207,9407,9607,9808,0008,0208,040
    attcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgataagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgactSspIAmpicillinbla (amp) promoter8,0608,0808,1008,1208,1408,1608,1808,2008,220
    agatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatatctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatAclIAmpicillin8,2408,2608,2808,3008,3208,3408,3608,3808,400
    cactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacagtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgtBcgIScaIAmpicillin8,4208,4408,4608,4808,5008,5208,5408,560
    acatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggatgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctAclIAmpicillin8,5808,6008,6208,6408,6608,6808,7008,7208,740
    ggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatgccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacAhdIAmpicillin8,7608,7808,8008,8208,8408,8608,8808,9008,920
    gatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcacAmpicillin8,9408,9608,9809,0009,0209,0409,0609,0809,100
    agttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagtcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcpUC ori9,1209,1409,1609,1809,2009,2209,2409,260
    cagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggatgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctapUC ori9,2809,3009,3209,3409,3609,3809,4009,4209,440
    aaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtpUC ori9,4609,4809,5009,5209,5409,5609,5809,6009,620
    cgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtaPciIpUC ori9,6409,6609,6809,7009,7209,7409,7609,7809,800
    gttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgccaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgSapIBspQI9,8209,8409,8609,8809,9009,9209,9409,960
    agctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgatttcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaa9,98010,00010,02010,04010,06010,08010,10010,12010,140
    acgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaagctttgcggttcgcgcgttaattgggagtgatttcccttgttttcgacctcgacgttcgaa10,16010,18010,200
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10207 bp750080008500900095005001000150020002500PcmvHIV-1 5' LTR

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    10207