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pDCAFA
  • Sequence Map
    Editing disabled on read-only sequence.
    tttacggctagctcagtcctaggtacaatgctagctactagagaaatcaaattaaggaggtaagataaatggaacaggcaatcattaatgatgaacgggagtatcttcgccatttctggcatcccgtatgtactgtaactgagcttgaaaaggcgcatccttccagcctcggcccaaatgccgatcgagtcaggatccatgttacgatcgatgatctctttagtttaattcctccattctatttaccttgtccgttagtaattactacttgccctcatagaagcggtaaagaccgtagggcatacatgacattgactcgaacttttccgcgtaggaaggtcggagccgggAseIBaeIBmtIBmtINheINheIAvrIIME2QA4II6ND8ER10EY12LR14HF16WH18PV20CT22VT24EL26EK28AH30PS32SL34GP3669-1124ndmA CDSndmA geneJ23110derived f...5105 RBSiGEM15-01C20406080100120140160
    cctggccgttaagctgctgaatgaacagctcgttgtcgccaagctaggcgatgagtacgtcgcgatgcgtgatagatgcgctcatcgatcagctaagctttccttgggtacagtaagtggaaaccgcctacagtgcccctatcacggatggcaatatgatacgcatggcgcttgcggaccggcaattcgacgacttacttgtcgagcaacagcggttcgatccgctactcatgcagcgctacgcactatctacgcgagtagctagtcgattcgaaaggaacccatgtcattcacctttggcggatgtcacggggatagtgcctaccgttatactatgcgtaccgcgaacgBlpINruILA38VK40LL42NE44QL46VV48AK50LG52DE54YV56AM58RD60RC62AH64RS66AK68LS70LG72TV74SG76NR78LQ80CP82YH84GW86QY88DT90HG92AC9469-1124ndmA CDSndmA gene180200220240260280300320340
    cagctcgtaccagcgtgccccaacagcccaatacccaacaaggctaaagttgatcgcttcgattgcgaagaacgctatggattgatttggattcgattggactctagttttgactgcactgaaattccctactttagtgcagccaacgatcctaggttgcgtattgttatacaaggtcgagcatggtcgcacggggttgtcgggttatgggttgttccgatttcaactagcgaagctaacgcttcttgcgatacctaactaaacctaagctaacctgagatcaaaactgacgtgactttaagggatgaaatcacgtcggttgctaggatccaacgcataacaatatgttcAvrIIQL96VP98AC100PN102SP104IP106NK108AK110VD112RF114DC116EE118RY120GL122IW124IR126LD128SS130FD132CT134EI136PY138FS140AA142ND144PR146LR148IV150IQ15269-1124ndmA CDSndmA gene360380400420440460480500520
    aaccttactggtgggatgcgactgcggaacgtagatgggaaaattttacagatttttctcactttgcattcattcacccaggcacgcttttcgatccaaataatgctgaacctccaattgttccgatggatcgattcaatggtcagtttcggtttgtctacgacacgccagaagattggaatgaccaccctacgctgacgccttgcatctacccttttaaaatgtctaaaaagagtgaaacgtaagtaagtgggtccgtgcgaaaagctaggtttattacgacttggaggttaacaaggctacctagctaagttaccagtcaaagccaaacagatgctgtgcggtcttctBcgIMfeIEP154YW156WD158AT160AE162RR164WE166NF168TD170FS172HF174AF176IH178PG180TL182FD184PN186NA188EP190PI192VP194MD196RF198NG200QF202RF204VY206DT208PE210D69-1124ndmA CDSndmA geneiGEM15-03CiGEM15-02C540560580600620640660680700
    tatggctgtcccaaatcaggctccaattggttcattttcgtatacttgcagcatgccgtttgctattaaccttgaagtatccaaatactccagcagttcgctgcatgtgttattcaatgtgtcatgtccggtagacagccacaccacgaaaaactttctgatcttcgctagggagataccgacagggtttagtccgaggttaaccaagtaaaagcatatgaacgtcgtacggcaaacgataattggaacttcataggtttatgaggtcgtcaagcgacgtacacaataagttacacagtacaggccatctgtcggtgtggtgctttttgaaagactagaagcgatccctcMfeISphIBstZ17IM212AV214PN216QA218PI220GS222FS224YT226CS228MP230FA232IN234LE236VS238KY240SS242SS244LH246VL248FN250VS252CP254VD256SH258TT260KN262FL264IF266AR268E69-1124ndmA CDSndmA gene720740760780800820840860
    caatcggacgactcggattatctgcacattgcatttaatgatctcgtcttcgctgaagacaaaccagtaattgagtcccaatggcctaaagacgcgccagcagatgaggtctcagtagtcgcagataaggtatcgatacaatatagaaaatggctgcgggaactaaaagaagctcgttagcctgctgagcctaatagacgtgtaacgtaaattactagagcagaagcgacttctgtttggtcattaactcagggttaccggatttctgcgcggtcgtctactccagagtcatcagcgtctattccatagctatgttatatcttttaccgacgcccttgattttcttcgagBbsIBbsIBsaIQ270SD272DS274DY276LH278IA280FN282DL284VF286AE288DK290PV292IE294SQ296WP298KD300AP302AD304EV306SV308VA310DK312VS314IQ316YR318KW320LR322EL324KE326A69-1124ndmA CDSndmA gene8809009209409609801,0001,0201,040
    ataaagaaggttcacaagccttccgaagtgctttgttagacccagtcattgaaagcgataggagctacatataatactagagtcacacaggaaagTACTAGatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatatttcttccaagtgttcggaaggcttcacgaaacaatctgggtcagtaactttcgctatcctcgatgtatattatgatctcagtgtgtcctttcATGATCtacttgtttgaactgcagttggtcaccaaaggataacgatggtgacttctagagggcttcgcggtacagaaagtBmrIBglIIPflFIHincIITsp45ITth111IMN2KL4DV6NQ8WF10PI12AT14TE16DL18PK20RH22VF24H1152-2918H328KE330GS332QA334FR336SA338LL340DP342VI344ES346DR348SY350I*35269-1124iGEM15-04CiGEM15-26CndmA CDSndmA genendmD CDSndmD geneBBa...321,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacggtgcaacaaccccgttctttaccggtagaccgcgctactgagaccaagtcaattacgaaccctcttggcgacgggcgtatctcctaacgccaactgtgacccacgattatggccattgctcaacgccacagtcatagtacctacctgaatactttcgccctgaccaccgacgAgeIBmrIMscIBstXIDraIIIHincIIA26TL28LG30QE32MA34IW36RD38DS40GS42VN44AW46EN48RC50PH52RG54LR56LT58LG60AN62TG64NE66LR68CQ70YH72GW74TY76ES78GT80GG82C1152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene1,2401,2601,2801,3001,3201,3401,3601,3801,400
    acttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatatgaaaacagggtcgggtagcgctacgtggtgggggtttacggcgcgcccaattatgaaaaggacaggcgcttttcgtgccgaaatagacctgctgtaatccagtcggcggtcctctcgggtaaagttaggagctactgcgagtcgaacatttgcgacatttttgtttagacgtatAhdIBlpINruIBseYIT84FV86PA88HR90DA92PP94PN96AA98RV100NT102FP104VR106EK108HG110FI112WT114TL116GQ118PP120GE122PI124SI126LD128DA130QL132VN134AV136KT138NL140H1152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene1,4201,4401,4601,4801,5001,5201,5401,560
    gcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatcgcatcaatatctacgactataactgcctcaacagtcgcaggatgcagtcttagaaagtcgcaagtagctacacaaaccacgccggtcgcgacttctagacgtaaactttaggtacgacgttctagctccctaagaccattgttctagtccgagataacgataagtaaaaatataAfeIDrdIBglIIS142VV144ID146AD148ID150GV152VS154VL156RQ158NL160SA162FI164DV166FG168AA170SA172ED174LH176LK178SM180LQ182DR184GI186LV188TR190SG192SI194AI196HF198YM2001152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaacgtcgcgagttggtaatcatttacgcaacatgtacgcgttcatgaatgaggccctgcaggtcctatgcttgaagttttcttgatgagcatacggtacttgcaataggcgtcccgtcgtcttcgacatcgatggctgaactaatcgtaatgtctatagtcgctagtctgatagcttAclIAfeIBsrGIEcoRVQR202ST204IS206KC208VV210HA212QV214LT216PG218RP220GY222EL224QK226NY228SY230AM232NV234IR236RA238AE240AV242AT244DL246IS248IT250DI252SD254QT256IE2581152-2918iGEM15-04CiGEM15-26CndmD CDSndmD geneiGEM15-24C1,7601,7801,8001,8201,8401,8601,8801,9001,920
    aagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggaggttcgaacttcagcaatctcttttgtactgattccgaggaggttgggtgatatagacgcttcaccaatgcgcatgagttctctgtccactataattgagtatgaccgacttcggatagccgatgggtgatggtcgtaagtcaggtccctacgtgtagtcgtagtgttgcggcctccMluIBsaBIBsaXIBseRIAflIIIKL260EV262VR264EN266MT268KA270PP272TH274YI276CE278VV280TR282TQ284ET286GD288IN290SY292WL294KP296IG298YP300LP302AF304SP306GM308HI310SI312TT314PE3161152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccatcgtaagctgttataagggagcacttgcccggactgctcgcacttaggaagtagtagccacacttctttctctaagtcagggcaccgccgaggtctagttacgtgcttctacacttccaaccttgcgttgattttcaatgtgaaggctccttgccaaaaggtgagcaggtttgGS318IR320QY322SL324VN326GP328DE330RE332SF334II336GV338KK340EI342QS344RG346GS348RS350MH352ED354VK356VG358TQ360LK362VT364LP366RN368GF370PL372VQ374T1152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene2,1202,1402,1602,1802,2002,2202,2402,260
    cagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatgtcttttgtgggctaagagcatcgtccaccgtagccatagtggggttaaaacacataccgtgtccgagacctagtcgttccaagtagcatactttatgtaataaaacgagcacgtaaactcgtacaaggtaaggtcctagctgactgacgcaacccgctagcaaacttacacgtaPvuIBfuAIBspMIPaqCIR376KH378PI380LV382AG384GI386GI388TP390IL392CM394AQ396AL398DQ400QG402SS404YE406IH408YF410AR412AF414EH416VP418FQ420DR422LT424AL426GD428RL430NV432H1152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtagaaccggagccgggtctgctctgatctcgttttgaacggctgtagtacctctaagtattgcgggttctgcatctacaaatgtgaacgccgggcgttggttactagctttgacatagacgacagcgagaacgagtaccgtagcgacttctcaggtaggctaaacttaaaaagtcatEarIBstAPIL434GL436GP438DE440TR442AK444LA446DI448ME450IH452NA454QD456VD458VY460TC462GP464QP466MI468ET470VS472AV474AL476AH478GI480AE482ES484IR486FE488FF490S1152-2918iGEM15-04CiGEM15-26CndmD CDSndmD geneiGEM15-25C2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtttttcttgctacaagggcaaagactacttcttatactccaactcgagtttttttgaccagtttataagtgacagagcggaccgagatgcaacaacgttcgaacaaacctgttgctacaagcatagcttcgaagaacactcgtcccacatacgccctgaacatattgaggtcagcaBarISacIEco53kIK492KN494DV496PV498SD500EE502YE504VE506LK508KT510GQ512IF514TV516SP518GS520TL522LQ524AC526LD528ND530VR532IE534AS536CE538QG540VC542GT544CI546TP548VV5501152-2918iGEM15-04CiGEM15-26CndmD CDSndmD gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaattaggccgctagagctcgtagtactgtgaatggaaagattctttctttccctttcgccattcacctagtacggcacacaaagcgcgacgttcaggtttttttagcaagagctagacactccggcccgtagtgggtgccatacctgctcgacatatttgatctctttctcctctttaPflMISG552DL554EH556HD558TY560LS562KK564ER566ES568GK570WI572MP574CV576SR578CK580SK582KI584VL586DL588*1152-2918iGEM15-04CiGEM15-26CndmD CDSndmD geneEQ_NdmD_pSB1C3_revEQ_gst9_1 (gBLOCK)EQ_pSB1C3_Gib_forBBa...BS2,8202,8402,8602,8802,9002,9202,9402,960
    actagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgccggtatttgatctactagtgcgaaatactgatacttgagagccccttaacgatgttccacgcctacgaggaaaggtaggacttcgatggcatgtgctatctctgtcaccttaaaaagggcaggtcccttgtatttagtggccggaaggacgcgtagttaggtgagccggtcaacggccataaBarIBclIBseRIMI2TL4YD6YE8LS10GN12CY14KV16RM18LL20SI22LK24LP26YT28IE30TV32EF34FP36SR38EH40KS42PA44FL46RI48NP50LG52QL54PV56L2981-3655iGEM15-04CiGEM15-26CEQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcgcgctactactaaagcacaacgcgctacgggtccggtaggaccaaatggaccgcttgtttatgctgtggtcaaactggaccataggctgtgtacgcgggcggtaggagccggtccgtttatacaccgagcgcaaacggctaccaaactggccgagctaaaggcgacgtgcaaacEcoNISexAIR58DD60DF62VL64RD66AQ68AI70LV72YL74AN76KY78DT80SL82TW84YP86TH88AP90AI92LG94QA96NM98WL100AF102AD104GL106TG108SI110SA112AR114L2981-3655iGEM15-04CiGEM15-26Cgst9 CDSgst9 geneEQ_gst9_1 (gBLOCK)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    catgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtgtactaaacaaaaaaatgctaaagttacgtctcgttacggcacgggcgcgcgtgcttgatgacgcgtatgacctgcttgtggacacccgccgccttgttttacctgtagttaccgatacaggccggcggcgtgggtggtagcgcctagaacggacgaagggcatatagcgtaacaBcgIBssHIIH116DL118FF120YD122FN124AE126QC128RA130RA132HE134LL136RI138LD140EH142LW144AA146EQ148NG150HQ152WL154CP156AA158AP160TI162AD164LA166CF168PY170IA172L2981-3655iGEM15-04CiGEM15-26Cgst9 CDSgst9 gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgatccggcctcacttgcggaagaaacctgtcaataatactagTAGCGGCCGCTGCagcctacttcggccgcaaagtgatgagctgatgggccgccatgctgcgaccgacctggcacagtttgcgtaggggcctaagtggcagtacagcccctaaaaaggttggagtcgaaggaagctaggccggagtgaacgccttctttggacagttattatgatcATCGCCGGCGACGtAjuINaeINotISpeIAvaIIBbvCIPflFINgoMIVTth111IS174DE176AG178VS180LL182DY184PA186VR188RW190LD192RV194KR196IP198GF200TV202MS204GI206FP208TS210AS212FD214PA216SL218AE220ET222CQ224*2981-3655iGEM15-26CiGEM15-04Cgst9 CDSgst9 geneiGEM15C-123,5203,5403,5603,5803,6003,6203,6403,660
    gtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggcaggccgttttttcccgttccacagtggtgggacgggaaaaagaaattttggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtccPstIBsrBITsp45IE. coli His Operon TerminatorVR PrimeriG...C3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaaDrdIPciIAflIIIrep (pMB1)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcgggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagApaLIBseYIrep (pMB1)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagttcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcarep (pMB1)4,2204,2404,2604,2804,3004,3204,3404,360
    atttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgactaaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgrep (pMB1)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggccgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcgagctccgaacctaagagtggttattttttgcgggccgPspXIT0 Terminator4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactaSacIEcoRVEco53kIChloramphenicol ResistanceT0 Terminator4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatcccttMscINcoIPasIBsmBIEsp3IPflMIChloramphenicol Resistance4,9204,9404,9604,9805,0005,0205,0405,060
    ataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgcctatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggAclIChloramphenicol Resistance5,0805,1005,1205,1405,1605,1805,2005,2205,240
    atacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgattatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctaBspEIPvuIIChloramphenicol Resistance5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctcggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacgggctagttgagctcacggtggactgcagaBsaAIPspXIChloramphenicol ResistanceVF2 ...mer5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagttctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgaaagcgattcctactaaagaccttaagcgccggcgaagatctcNotIXbaIEcoRIStem LoopiGEM15-01CPrefix5,6205,6405,6605,6805,7005,720
    • Plasmid
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    Editing disabled on read-only sequence.
    5725 bp42504500475050005250550025050075010001250iGEM15-26CiGEM15-04CndmD CDSndmD genendmA CDSndmA genepoint mutationiGEM15-01C1152-291869-1124NheIJ23110,derived from BBa K515105 RBS,BmtI,AvrII,NheI,BmtI,BaeI,AseI,NruI,+1iGEM15-02C,iGEM15-03C,AvrIIBcgI,MfeIMfeIBstZ17I,SphIBbsI,BbsIBsaIBmrIBBa_B0032,PflFI,Tth111I,Tsp45IHincII,BglIIBstXI,MscIHincII,DraIII,BmrI,AgeIBseYI,NruIPspXIT0 TerminatorEco53kISacI,EcoRVNcoI,MscI,PasI,PflMI,BsmBI,Esp3IChloramphenicol ResistanceAclI,BspEIPvuIIVF2 Primer,End of Terminator,Stem Loop,Prefix,BsaAI,PspXI,EcoRI,NotI,XbaI

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    5725