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pFYF_EMX1
  • Sequence Map
    Editing disabled on read-only sequence.
    gacgtcgctagctgtacaaaaaagcaggctttaaaggaaccaattcagtcgactggatccggtaccaaggtcgggcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagactgcagcgatcgacatgttttttcgtccgaaatttccttggttaagtcagctgacctaggccatggttccagcccgtccttctcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaatcttaattaaactgacatttgtgtttctAccIAseIBaeIBmtIEarIKpnINheISalIStyITfiIXmnIZraIAatIIBamHIBsaHIBsaJIBsrGIAcc65IHincIIEcoO109IU6 promoter20406080100120140160
    tattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgagtccgagcagaagaagaaataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaacacctttcctgctttgtggctcaggctcgtcttcttcttNdeIBsaAIU6 promoterUniversal reverseEMX1 pr...spacerFull EM...primersgRNAGGCACG...GCCGG180200220240260280300320340
    gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttaagcttgggccgctcgaggtacctctctacatatgacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaattcgaacccggcgagctccatggagagatgtatactgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccAvaIKpnINdeINspIPciITliIXhoIBsoBIBsrBIPspXIAcc65IAflIIIPaeR7IHindIIIsgRNAFull EMX1 forward primerUniversal forw...ealing region360380400420440460480500520
    cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggDrdIMmeIBciVIBsaJIBssSIBssSαI540560580600620640660680700
    ttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactgaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgacMmeIApaLIBaeGIBseYIHaeII720740760780800820840860
    gcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttAcuIAlwNIBceAI8809009209409609801,0001,0201,040
    ccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaBspHI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtAhdIAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaatAseIBglIBmrIBpmIBsaIAvaIIBcoDIBsmAIBsrDIBsrFINmeAIIIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    atagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagtttatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaAclIFspIPvuIAvaIIBsrDIAmpR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtBcgIBtsIBtsIEaeIScaIBsaHIBtsαIBtsαIAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    catagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaagtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgtttAclIAcuIXmnIApaLIBaeGIBssSIBssSαIAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    atgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgcctacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggEarISspIBciVIBcoDIBsmAIBspHIBsrBIAmpRamp promoter2,1202,1402,1602,1802,2002,2202,2402,260
    accttgga2,279
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    2279 bp150017502000250500AmpRsgRNAFull EMX1 forward primerGGCACGGGCAGCTTGCCGGBsaHI,ZraI,AatII,NheI,BmtI,BsrGI,XmnI,SalI,AccI,HincII,BamHI,Acc65I,BsaJI,KpnI,+5AseIU6 promoter,BsaAINdeIUniversal reverse,EMX1 protospacerUniversal forward annealing regionHindIII,BsrBI,AvaI,BsoBI,PaeR7I,PspXI,TliI,XhoI,Acc65I,KpnI,NdeIAflIIIPciINspIAvaII,PvuIEaeI,BtsI,BtsαIBtsI,BtsαIScaIBcgIBsaHIAclIXmnIBssSIBssSαI,AcuI,ApaLI,BaeGIamp promoter,EarI,SspI,BspHI,BciVI,BcoDI,BsmAI,BsrBI

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    2279