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pHR_SpCas9
  • Sequence Map
    Editing disabled on read-only sequence.
    ctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttgactgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaNaeINgoMIVF1 ori20406080100120140160
    ccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctatggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataDrdIF1 ori180200220240260280300320340
    tcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaaggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcApoIApoIPsiISspILacZ alpha360380400420440460480500520
    ccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagcaccgcggtggcggccgctctagagacggcggggagctcgctgaggctggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacctcgtggcgccaccgccggcgagatctctgccgcccctcgagcgactccgaAleINotISacIXbaIBbvCIEco53kIT7M13-fwd540560580600620640660680700
    tgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactgtactaaccacgcatacaaacatacttcgatgtcctgactaaaccgcccgatactcccgcccccttcgagaccttcccggcgctaccccgcgcgccgcaggtcttccgcggtatgccgggcgaccgccgtgggtaggccatattttcgggcgctggggcttgccactggaggBstEII720740760780800820840860
    actttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagtgaaagtcgctgtttgctcgtgaatatgtatgcgctgataagacggcgatatgtattggtgagtcgatcgaattctagggtagttcgaacgtacggcccgcgcggtcttcctcgcgtcggtttggtcctactacaaactaccccataaactcgtgaacgttgggaataggccttcAscIBmtINheIAflIIBspEIHindIIIRBCS2 Promotor5´UTR HSP70A8809009209409609801,0001,0201,040
    ccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaaatggccaggtgagtcgacgagcaagcccggcggatcaggcgggggaccgggtgtttccgatccgcggttacgttcgtcaagcgtacgtcggggacctcgccacgggaggactatttggccggtcccccggatacaagaaatgaaaaaatgttctcttcagtgagttgtagaattttaccggtccactcagctgctcgttcgggccgcctagtccgAccISalIHincIIRBCS2 PromotorIntron 15´UTR RBCS21,0601,0801,1001,1201,1401,1601,1801,2001,220
    agcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcaggatgctcgagATGGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAtcgcacgaacgtctaaactgaacgttgcgggcgtaacacagctgcttccgaaaaccgaggagacagcgacagagttcgtcgtagattgggacgcagcggcaaaggtaaacgtcctacgagctcTACCTGATGTTCCTGGTGCTGCCGCTGATGTTCCTGGTGCTGTAGCTGATGTAccISalITliIXhoIHincIIPaeR7IM1D2Y3K41349-1361M1D2Y3K4D5H6D7G8D9Y10K11D12H13D14I15D16Y171349-1468Intron 1FLAG+NLS1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AGGACGACGACGACAAGATGGCCCCGAAGAAGAAGCGCAAGGTCGGCATCCACGGCGTGCCCGCCGCGgacaagaagtactccatcggcctggacatcggcacgaacagcgtgggctgggccgtgatcaccgacgagtacaaggtgccgtccaagaagttcaaggtgctgggcaaTCCTGCTGCTGCTGTTCTACCGGGGCTTCTTCTTCGCGTTCCAGCCGTAGGTGCCGCACGGGCGGCGCctgttcttcatgaggtagccggacctgtagccgtgcttgtcgcacccgacccggcactagtggctgctcatgttccacggcaggttcttcaagttccacgacccgttBstXIK18D19D20D21D22K23M24A25P26K27K28K29R30K31V32G33I34H35G36V37P38A39A401349-1468DK2KY4SI6GL8DI10GT12NS14VG16WA18VI20TD22EY24KV26PS28KK30FK32VL34GN361469-5620Cas9\chlamyFLAG+NLS1,4201,4401,4601,4801,5001,5201,5401,560
    caccgaccgccacagcatcaagaagaacctgatcggcgccctgctgttcgactcgggcgagaccgccgaggcgacgcgcctgaagcgcaccgcgcgccgccgctacacgcgccgcaagaaccgcatctgctacctgcaggagatcttcagcaacgagatggccaaggtggacgacgtggctggcggtgtcgtagttcttcttggactagccgcgggacgacaagctgagcccgctctggcggctccgctgcgcggacttcgcgtggcgcgcggcggcgatgtgcgcggcgttcttggcgtagacgatggacgtcctctagaagtcgttgctctaccggttccacctgctgSbfITD38RH40SI42KK44NL46IG48AL50LF52DS54GE56TA58EA60TR62LK64RT66AR68RR70YT72RR74KN76RI78CY80LQ82EI84FS86NE88MA90KV92DD941469-5620Cas9\chlamy1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcgttcttccaccgcctggaggagtccttcctggtggaggaggacaagaagcacgagcgccaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtacccgacgatctaccacctgcgcaagaagctggtggacagcaccgacaaggcggacctgcgcctgaagcaagaaggtggcggacctcctcaggaaggaccacctcctcctgttcttcgtgctcgcggtggggtagaagccgttgtagcacctgctccaccggatggtgctcttcatgggctgctagatggtggacgcgttcttcgaccacctgtcgtggctgttccgcctggacgcggactPaqCISF96FH98RL100EE102SF104LV106EE108DK110KH112ER114HP116IF118GN120IV122DE124VA126YH128EK130YP132TI134YH136LR138KK140LV142DS144TD146KA148DL150RL1521469-5620Cas9\chlamyMut11,7601,7801,8001,8201,8401,8601,8801,9001,920
    tctacctggccctggcgcacatgatcaagttccgcggccacttcctgatcgagggcgacctgaaccccgacaactcggacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggagaacccgatcaacgcctcgggcgtggacgccaaggcgatcctagatggaccgggaccgcgtgtactagttcaaggcgccggtgaaggactagctcccgctggacttggggctgttgagcctgcacctgttcgacaagtaggtcgaccacgtctggatgttggtcgacaagctcctcttgggctagttgcggagcccgcacctgcggttccgctaggaIY154LA156LA158HM160IK162FR164GH166FL168IE170GD172LN174PD176NS178DV180DK182LF184IQ186LV188QT190YN192QL194FE196EN198PI200NA202SG204VD206AK208AI210L1469-5620Cas9\chlamy1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gtccgcgcgcctgtccaagagccgccgcctggagaacctgatcgcccagctgcccggcgagaagaagaacggcctgttcggcaacctgatcgcgctgagcctgggcctgacgccgaacttcaagtcgaacttcgacctggccgaggacgcgaagctgcagctgagcaaggacacccaggcgcgcggacaggttctcggcggcggacctcttggactagcgggtcgacgggccgctcttcttcttgccggacaagccgttggactagcgcgactcggacccggactgcggcttgaagttcagcttgaagctggaccggctcctgcgcttcgacgtcgactcgttcctgtggS212AR214LS216KS218RR220LE222NL224IA226QL228PG230EK232KN234GL236FG238NL240IA242LS244LG246LT248PN250FK252SN254FD256LA258ED260AK262LQ264LS266KD268T1469-5620Cas9\chlamy2,1202,1402,1602,1802,2002,2202,2402,260
    tacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgcggacctgttcctggccgcgaagaacctgtcggacgccatcctgctgtccgacatcctgcgcgtgaacaccgagatcacgaaggcccccctgtcggcgtccatgatcaagcgctacgacgagcaccatgctgctgctggacctgttggacgaccgggtctagccgctggtcatgcgcctggacaaggaccggcgcttcttggacagcctgcggtaggacgacaggctgtaggacgcgcacttgtggctctagtgcttccggggggacagccgcaggtactagttcgcgatgctgctcgtggAfeIY270DD272DL274DN276LL278AQ280IG282DQ284YA286DL288FL290AA292KN294LS296DA298IL300LS302DI304LR306VN308TE310IT312KA314PL316SA318SM320IK322RY324DE326H1469-5620Cas9\chlamy2,2802,3002,3202,3402,3602,3802,4002,4202,440
    accaggacctgaccctgctgaaggcgctggtgcgccagcagctgccggagaagtacaaggagatcttcttcgaccagtccaagaacggctacgccggctacatcgacggcggcgccagccaggaggagttctacaagttcatcaagcccatcctggagaagatggacggcacggatggtcctggactgggacgacttccgcgaccacgcggtcgtcgacggcctcttcatgttcctctagaagaagctggtcaggttcttgccgatgcggccgatgtagctgccgccgcggtcggtcctcctcaagatgttcaagtagttcgggtaggacctcttctacctgccgtgcctNaeINgoMIVH328QD330LT332LL334KA336LV338RQ340QL342PE344KY346KE348IF350FD352QS354KN356GY358AG360YI362DG364GA366SQ368EE370FY372KF374IK376PI378LE380KM382DG384TE3861469-5620Cas9\chlamy2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggagctgctggtgaagctgaaccgcgaggacctgctgcgcaagcagcgcaccttcgacaacggcagcatcccccaccagatccacctgggcgagctgcacgccatcctgcgccgccaggaggacttctacccgttcctgaaggacaaccgcgagaagatcgagaagatcctgacgcctcgacgaccacttcgacttggcgctcctggacgacgcgttcgtcgcgtggaagctgttgccgtcgtagggggtggtctaggtggacccgctcgacgtgcggtaggacgcggcggtcctcctgaagatgggcaaggacttcctgttggcgctcttctagctcttctaggactgcEL388LV390KL392NR394ED396LL398RK400QR402TF404DN406GS408IP410HQ412IH414LG416EL418HA420IL422RR424QE426DF428YP430FL432KD434NR436EK438IE440KI442LT4441469-5620Cas9\chlamy2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ttccgcatcccctactacgtgggcccgctggctcgcggcaactcccgcttcgcgtggatgacccgcaagagcgaggagaccatcacgccctggaacttcgaggaggtggtggacaagggcgccagcgcgcagtcgttcatcgagcgcatgaccaacttcgacaagaacctgcccaaaggcgtaggggatgatgcacccgggcgaccgagcgccgttgagggcgaagcgcacctactgggcgttctcgctcctctggtagtgcgggaccttgaagctcctccaccacctgttcccgcggtcgcgcgtcagcaagtagctcgcgtactggttgaagctgttcttggacgggtApaIPspOMIFR446IP448YY450VG452PL454AR456GN458SR460FA462WM464TR466KS468EE470TI472TP474WN476FE478EV480VD482KG484AS486AQ488SF490IE492RM494TN496FD498KN500LP5021469-5620Cas9\chlamy2,8202,8402,8602,8802,9002,9202,9402,960
    acgagaaggtgctgccgaagcactccctgctgtacgagtacttcaccgtgtacaacgagctgacgaaggtgaagtacgtgaccgagggcatgcgcaagcccgccttcctgagcggcgagcagaagaaggcgatcgtggacctgctgttcaagaccaaccgcaaggtgacggtgaatgctcttccacgacggcttcgtgagggacgacatgctcatgaagtggcacatgttgctcgactgcttccacttcatgcactggctcccgtacgcgttcgggcggaaggactcgccgctcgtcttcttccgctagcacctggacgacaagttctggttggcgttccactgccacttBsrGIBstAPINE504KV506LP508KH510SL512LY514EY516FT518VY520NE522LT524KV526KY528VT530EG532MR534KP536AF538LS540GE542QK544KA546IV548DL550LF552KT554NR556KV558TV560K1469-5620Cas9\chlamy2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gcagctgaaggaggactacttcaagaagatcgagtgcttcgacagcgtggagatctcgggcgtggaggaccgcttcaacgccagcctgggcacctaccacgacctgctgaagatcatcaaggacaaggacttcctggacaacgaggagaacgaggacatcctggaggacatcgtgcgtcgacttcctcctgatgaagttcttctagctcacgaagctgtcgcacctctagagcccgcacctcctggcgaagttgcggtcggacccgtggatggtgctggacgacttctagtagttcctgttcctgaaggacctgttgctcctcttgctcctgtaggacctcctgtagcacQ562LK564ED566YF568KK570IE572CF574DS576VE578IS580GV582ED584RF586NA588SL590GT592YH594DL596LK598II600KD602KD604FL606DN608EE610NE612DI614LE616DI618V1469-5620Cas9\chlamy3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctgaccctgacgctgttcgaggaccgcgagatgatcgaggagcgcctgaagacgtacgcccacctgttcgacgacaaggtgatgaagcagctgaagcgccgccgctacaccggctggggccgcctgagccgcaagctgatcaacggcatccgcgacaagcagtcgggcaagaccagactgggactgcgacaagctcctggcgctctactagctcctcgcggacttctgcatgcgggtggacaagctgctgttccactacttcgtcgacttcgcggcggcgatgtggccgaccccggcggactcggcgttcgactagttgccgtaggcgctgttcgtcagcccgttctggtAhdIBbsIBsiWIL620TL622TL624FE626DR628EM630IE632ER634LK636TY638AH640LF642DD644KV646MK648QL650KR652RR654YT656GW658GR660LS662RK664LI666NG668IR670DK672QS674GK676T1469-5620Cas9\chlamy3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tcctggacttcctgaagtccgacggcttcgcgaaccgcaacttcatgcagctgatccacgacgactcgctgaccttcaaggaggacatccagaaggcccaggtgtcgggccagggcgactccctgcacgagcacatcgccaacctggcgggctcccccgcgatcaagaagggcataggacctgaaggacttcaggctgccgaagcgcttggcgttgaagtacgtcgactaggtgctgctgagcgactggaagttcctcctgtaggtcttccgggtccacagcccggtcccgctgagggacgtgctcgtgtagcggttggaccgcccgagggggcgctagttcttcccgtaNruII678LD680FL682KS684DG686FA688NR690NF692MQ694LI696HD698DS700LT702FK704ED706IQ708KA710QV712SG714QG716DS718LH720EH722IA724NL726AG728SP730AI732KK734GI7361469-5620Cas9\chlamy3,5203,5403,5603,5803,6003,6203,6403,660
    cctgcagaccgtgaaggtggtggacgagctggtgaaggtgatgggccgccacaagccggagaacatcgtgatcgagatggcccgcgagaaccagaccacgcagaagggccagaagaacagccgcgagcgcatgaagcgcatcgaggagggcatcaaggagctgggctcgcagatcggacgtctggcacttccaccacctgctcgaccacttccactacccggcggtgttcggcctcttgtagcactagctctaccgggcgctcttggtctggtgcgtcttcccggtcttcttgtcggcgctcgcgtacttcgcgtagctcctcccgtagttcctcgacccgagcgtctagLQ738TV740KV742VD744EL746VK748VM750GR752HK754PE756NI758VI760EM762AR764EN766QT768TQ770KG772QK774NS776RE778RM780KR782IE784EG786IK788EL790GS792QI7941469-5620Cas9\chlamy3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctgaaggagcaccccgtggagaacacccagctgcagaacgagaagctgtacctgtactacctgcagaacggccgcgacatgtacgtggaccaggagctggacatcaaccgcctgtccgactacgacgtggaccacatcgtgccccagagcttcctgaaggacgactcgatcgacagacttcctcgtggggcacctcttgtgggtcgacgtcttgctcttcgacatggacatgatggacgtcttgccggcgctgtacatgcacctggtcctcgacctgtagttggcggacaggctgatgctgcacctggtgtagcacggggtctcgaaggacttcctgctgagctagctgtPciILK796EH798PV800EN802TQ804LQ806NE808KL810YL812YY814LQ816NG818RD820MY822VD824QE826LD828IN830RL832SD834YD836VD838HI840VP842QS844FL846KD848DS850ID8521469-5620Cas9\chlamy3,8603,8803,9003,9203,9403,9603,9804,0004,020
    acaaggtgctgacccgcagcgacaagaaccgcggcaagagcgacaacgtgccgtcggaggaggtggtgaagaagatgaagaactactggcgccagctgctgaacgccaagctgatcacgcagcgcaagttcgacaacctgaccaaggccgagcgcggcggcctgtcggagctggatgttccacgactgggcgtcgctgttcttggcgccgttctcgctgttgcacggcagcctcctccaccacttcttctacttcttgatgaccgcggtcgacgacttgcggttcgactagtgcgtcgcgttcaagctgttggactggttccggctcgcgccgccggacagcctcgacctNK854VL856TR858SD860KN862RG864KS866DN868VP870SE872EV874VK876KM878KN880YW882RQ884LL886NA888KL890IT892QR894KF896DN898LT900KA902ER904GG906LS908EL910D1469-5620Cas9\chlamy4,0404,0604,0804,1004,1204,1404,1604,1804,200
    caaggcgggcttcatcaagcgccagctggtggagacccgccagatcacgaagcacgtggcgcagatcctggactcccgcatgaacacgaagtacgacgagaacgacaagctgatccgcgaggtgaaggtgatcaccctgaagtccaagctggtcagcgacttccgcaaggacttcgttccgcccgaagtagttcgcggtcgaccacctctgggcggtctagtgcttcgtgcaccgcgtctaggacctgagggcgtacttgtgcttcatgctgctcttgctgttcgactaggcgctccacttccactagtgggacttcaggttcgaccagtcgctgaaggcgttcctgaagPmlIK912AG914FI916KR918QL920VE922TR924QI926TK928HV930AQ932IL934DS936RM938NT940KY942DE944ND946KL948IR950EV952KV954IT956LK958SK960LV962SD964FR966KD968F1469-5620Cas9\chlamy4,2204,2404,2604,2804,3004,3204,3404,360
    cagttctacaaggtgcgcgagatcaacaactaccaccacgcccacgacgcgtacctgaacgccgtggtgggcaccgcgctgatcaagaagtaccccaagctggagagcgagttcgtgtacggcgactacaaggtgtacgacgtgcgcaagatgatcgccaagtcggagcaggagagtcaagatgttccacgcgctctagttgttgatggtggtgcgggtgctgcgcatggacttgcggcaccacccgtggcgcgactagttcttcatggggttcgacctctcgctcaagcacatgccgctgatgttccacatgctgcacgcgttctactagcggttcagcctcgtcctctMluIQ970FY972KV974RE976IN978NY980HH982AH984DA986YL988NA990VV992GT994AL996IK998KY1000PK1002LE1004SE1006FV1008YG1010DY1012KV1014YD1016VR1018KM1020IA1022KS1024EQ1026E1469-5620Cas9\chlamy4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tcggcaaggccaccgcgaagtacttcttctactccaacatcatgaacttcttcaagaccgagatcacgctggccaacggcgagatccgcaagcgccccctgatcgagaccaacggcgagacgggcgagatcgtgtgggacaagggccgcgacttcgcgaccgtgcgcaaggtgctagccgttccggtggcgcttcatgaagaagatgaggttgtagtacttgaagaagttctggctctagtgcgaccggttgccgctctaggcgttcgcgggggactagctctggttgccgctctgcccgctctagcacaccctgttcccggcgctgaagcgctggcacgcgttccacgaNruII1028GK1030AT1032AK1034YF1036FY1038SN1040IM1042NF1044FK1046TE1048IT1050LA1052NG1054EI1056RK1058RP1060LI1062ET1064NG1066ET1068GE1070IV1072WD1074KG1076RD1078FA1080TV1082RK1084VL10861469-5620Cas9\chlamy4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gagcatgccccaggtgaacatcgtgaagaagaccgaggtgcagacgggcggcttctccaaggagagcatcctgccgaagcgcaactcggacaagctgatcgcccgcaagaaggactgggaccccaagaagtacggcggcttcgactccccgaccgtggcctacagcgtgctggtgctcgtacggggtccacttgtagcacttcttctggctccacgtctgcccgccgaagaggttcctctcgtaggacggcttcgcgttgagcctgttcgactagcgggcgttcttcctgaccctggggttcttcatgccgccgaagctgaggggctggcaccggatgtcgcacgaccacBbsIKflISM1088PQ1090VN1092IV1094KK1096TE1098VQ1100TG1102GF1104SK1106ES1108IL1110PK1112RN1114SD1116KL1118IA1120RK1122KD1124WD1126PK1128KY1130GG1132FD1134SP1136TV1138AY1140SV1142LV11441469-5620Cas9\chlamy4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtggcgaaggtggagaagggcaagtccaagaagctgaagagcgtgaaggagctgctgggcatcaccatcatggagcgcagctcgttcgagaagaaccccatcgacttcctggaggcgaagggctacaaggaggtgaagaaggacctgatcatcaagctgccgaagtactcgctgtcaccgcttccacctcttcccgttcaggttcttcgacttctcgcacttcctcgacgacccgtagtggtagtacctcgcgtcgagcaagctcttcttggggtagctgaaggacctccgcttcccgatgttcctccacttcttcctggactagtagttcgacggcttcatgagcgacaSapIXcmIBspQIVA1146KV1148EK1150GK1152SK1154KL1156KS1158VK1160EL1162LG1164IT1166IM1168ER1170SS1172FE1174KN1176PI1178DF1180LE1182AK1184GY1186KE1188VK1190KD1192LI1194IK1196LP1198KY1200SL12021469-5620Cas9\chlamy4,9204,9404,9604,9805,0005,0205,0405,060
    tcgagctggagaacggccgcaagcgcatgctggcctccgcgggcgagctgcagaagggcaacgagctggccctgcccagcaagtacgtgaacttcctgtacctggcgtcccactacgagaagctgaagggctcgccggaggacaacgagcagaagcagctgttcgtggagcagcaagctcgacctcttgccggcgttcgcgtacgaccggaggcgcccgctcgacgtcttcccgttgctcgaccgggacgggtcgttcatgcacttgaaggacatggaccgcagggtgatgctcttcgacttcccgagcggcctcctgttgctcgtcttcgtcgacaagcacctcgtcgtFE1204LE1206NG1208RK1210RM1212LA1214SA1216GE1218LQ1220KG1222NE1224LA1226LP1228SK1230YV1232NF1234LY1236LA1238SH1240YE1242KL1244KG1246SP1248ED1250NE1252QK1254QL1256FV1258EQ1260H1469-5620Cas9\chlamyMut25,0805,1005,1205,1405,1605,1805,2005,2205,240
    caagcactacctggacgagatcatcgagcagatctcggagttctccaagcgcgtgatcctggccgacgcgaacctggacaaggtgctgagcgcctacaacaagcaccgcgacaagcccatccgcgagcaggcggagaacatcatccacctgttcaccctgacgaacctgggcgccgttcgtgatggacctgctctagtagctcgtctagagcctcaagaggttcgcgcactaggaccggctgcgcttggacctgttccacgactcgcggatgttgttcgtggcgctgttcgggtaggcgctcgtccgcctcttgtagtaggtggacaagtgggactgcttggacccgcggK1262HY1264LD1266EI1268IE1270QI1272SE1274FS1276KR1278VI1280LA1282DA1284NL1286DK1288VL1290SA1292YN1294KH1296RD1298KP1300IR1302EQ1304AE1306NI1308IH1310LF1312TL1314TN1316LG1318A1469-5620Cas9\chlamy5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ccggccgcgttcaagtacttcgacaccacgatcgaccgcaagcgctacaccagcacgaaggaggtgctggacgcgaccctgatccaccagagcatcaccggcctgtacgagacgcgcatcgacctgtcgcagctgggcggcgacaagcgcccggcggcgaccaagaaggcgggccggccggcgcaagttcatgaagctgtggtgctagctggcgttcgcgatgtggtcgtgcttcctccacgacctgcgctgggactaggtggtctcgtagtggccggacatgctctgcgcgtagctggacagcgtcgacccgccgctgttcgcgggccgccgctggttcttccgcccggAfeIP1320AA1322FK1324YF1326DT1328TI1330DR1332KR1334YT1336ST1338KE1340VL1342DA1344TL1346IH1348QS1350IT1352GL1354YE1356TR1358ID1360LS1362QL1364GG1366DK1368RP1370AA1372TK1374KA1376G1469-5620Cas9\chlamyNLS5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aggcgaagaagaagaagtaaggatccccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggatccgcttcttcttcttcattcctaggggcgaggcacatttacctccgcgagcaactagactcggaacgggggactgcttgccgccacctaccttctatgacgagagttcacgacttcgccatcgaatcgaggggcaaagcacgactagtcagaaaaagttgtgcatttttcgcctBamHIQ1378AK1380KK1382K*13841469-5620Rbcs2\Term.Cas9\chlamyNLS5,6205,6405,6605,6805,7005,7205,7405,760
    ggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcgggtacccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatcccctcaaaacgttaaaacaaccaacattgctaggaggcaactaaaaccggagaaagaggtacccgcccgacccgcataaacttcgcccatgggtcgaaaacaagggaaatcactcccaattaacgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggKpnINcoIXcmIAcc65IRbcs2\Term.M13-revT3T3 promoterLacO5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacTfiIlac promoterLacO5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaagcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcattttPciISapITfiIBspQI6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggataccttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggaDrdIBciVIColE1 origin6,3206,3406,3606,3806,4006,4206,4406,460
    gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacaccaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgApaLIColE1 origin6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaaColE1 origin6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatctaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctaColE1 origin6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatAhdIAmpR7,0207,0407,0607,0807,1007,1207,1407,160
    cgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattBsrDIAmpR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgtcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaAclIBsrDIAmpR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccAmpR7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaactattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgttAclIApaLIAmpR7,7207,7407,7607,7807,8007,8207,8407,860
    aaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaSspIBciVI7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tccccgaaaagtgccacaggggcttttcacggtg8,060
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8067 bp6000650070007500500100015002000Cas9\chlamyMut11349-13611349-14681469-5620D10A SpCas9 F (DNA)SpCas9 D10A R (DNA)F1 ori,NgoMIV,NaeI,DrdI,PsiILacZ alpha,M13-fwd,T7,ApoI,ApoI,SspIAleI,NotI,XbaI,Eco53kI,SacI,BbvCI5´UTR HSP70A,BstEII,NheI,BmtI,AflII,HindIII,AscIRBCS2 Promotor,BspEI5´UTR RBCS2,SalI,AccI,HincIIIntron 1,SalI,AccI,HincII,PaeR7I,TliI,XhoIFLAG+NLSBstXISbfIPaqCITfiI,BspQI,SapITfiIPciIDrdI,BciVIApaLIColE1 originAhdI,BsrDIAclIAmpR,BsrDIAclI,ApaLI,SspI,BciVI

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    8067