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pGRN_H-pU6:pPIN1_gRNA_Target1-tU6
  • Sequence Map
    Editing disabled on read-only sequence.
    ctaatcggagtacgtgaaatcgctaataaggtcacgtgaaatcgctaatcaaaaaggcacgtgagaacgctaatagccctttcagatcaacagcttgcaaacacccctcgctccggcaagtagttacagcaagtagtatgttcaattagcttttcaattatgaatatatatatcagattagcctcatgcactttagcgattattccagtgcactttagcgattagtttttccgtgcactcttgcgattatcgggaaagtctagttgtcgaacgtttgtggggagcgaggccgttcatcaatgtcgttcatcatacaagttaatcgaaaagttaatacttatatatatagt20406080100120140160
    attattggtcgcccttggcttgtggacaatgcgctacgcgcaccggctccgcccgtggacaaccgcaagcggttgcccaccgtcgagcgccagcgcctttgcccacaacccggcggccggccgcaacagatcgttttataaatttttttttttgaaaaagaaaaagcccgaaaggtaataaccagcgggaaccgaacacctgttacgcgatgcgcgtggccgaggcgggcacctgttggcgttcgccaacgggtggcagctcgcggtcgcggaaacgggtgttgggccgccggccggcgttgtctagcaaaatatttaaaaaaaaaaactttttctttttcgggctttccBglIFseINaeIPsiIStyINgoMIV180200220240260280300320340
    cggcaacctctcgggcttctggatttccgatccccggaattagaagatcttggcaggatatattgtggtgtaacgttatcgatctggattttagtactggattttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactaagggtttcttagccgttggagagcccgaagacctaaaggctaggggccttaatcttctagaaccgtcctatataacaccacattgcaatagctagacctaaaatcatgacctaaaaccaaaatccttaatctttaaaataactatcttcataaaatgtttatgtttatgtatgattcccaaagaatAclIAvaIClaIScaIBsoBIBspDILB360380400420440460480500520
    tatgctcaacacatgagcgaaaccctataagaaccctaatttcccttatcgggaaactactcacacattatttatggagaaaatagagagagatagatttgtagagagagactggtgatttcagcgtaccgaattaattctcccgcgaccagccgagcgagcttagcgaactgtgatacgagttgtgtactcgctttgggatattcttgggattaaagggaatagccctttgatgagtgtgtaataaatacctcttttatctctctctatctaaacatctctctctgaccactaaagtcgcatggcttaattaagagggcgctggtcggctcgctcgaatcgcttgacacBcgIBlpIXmnI540560580600620640660680700
    gacgagaactgtgccaccaagcgtaaggccgttctctcgcatttgccttgctaggctcgcgcgagttgctggctgaggcgttctcgaaatcagctcttgttcggtcggcatctactctattcctttgccctcggacgagtgctggggcgtcggtttccactatcggcgagtacttctgctcttgacacggtggttcgcattccggcaagagagcgtaaacggaacgatccgagcgcgctcaacgaccgactccgcaagagctttagtcgagaacaagccagccgtagatgagataaggaaacgggagcctgctcacgaccccgcagccaaaggtgatagccgctcatgaaBplIBplIScaIBbvCIBsaHIBpu10INmeAIIIHgR720740760780800820840860
    ctacacagccatcggtccagacggccgcgcttctgcgggcgatttgtgtacgcccgacagtcccggctccggatcggacgattgcgtcgcatcgaccctgcgcccaagctgcatcatcgaaattgccgtcaaccaagctctgatagagttggtcaagaccaatgcggagcatatagatgtgtcggtagccaggtctgccggcgcgaagacgcccgctaaacacatgcgggctgtcagggccgaggcctagcctgctaacgcagcgtagctgggacgcgggttcgacgtagtagctttaacggcagttggttcgagactatctcaaccagttctggttacgcctcgtatatHincIIHgR8809009209409609801,0001,0201,040
    cgcccggaggcgtggcgatcctgcaagctccggatgcctccgctcgaagtagcgcgtctgctgctccatacaagccaaccacggcctccagaagaagatgttggcgacctcgtattgggaatccccgaacatcgcctcgctccagtcaatgaccgctgttatgcggccattgtccgcgggcctccgcaccgctaggacgttcgaggcctacggaggcgagcttcatcgcgcagacgacgaggtatgttcggttggtgccggaggtcttcttctacaaccgctggagcataacccttaggggcttgtagcggagcgaggtcagttactggcgacaatacgccggtaacaggBtgZIHgR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gtcaggacattgttggagccgaaatccgcgtgcacgaggtgccggacttcggggcagtcctcggcccaaagcatcagctcatcgagagcctgcgcgacggacgcactgacggtgtcgtccatcacagtttgccagtgatacacatggggatcagcaatcgcgcatatgaaatcaccagtcctgtaacaacctcggctttaggcgcacgtgctccacggcctgaagccccgtcaggagccgggtttcgtagtcgagtagctctcggacgcgctgcctgcgtgactgccacagcaggtagtgtcaaacggtcactatgtgtacccctagtcgttagcgcgtatactttagtgNdeIPflFIBstAPINmeAIIITth111IHgR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gccatgtagtgtattgaccgattccttgcggtccgaatgggccgaacccgctcgtctggctaagatcggccgcagcgatcgcatccatagcctccgcgaccggctgaagaacagcgggcagttcggtttcaggcaggtcttgcaacgtgacaccctgtgcacggcgggagatgcacggtacatcacataactggctaaggaacgccaggcttacccggcttgggcgagcagaccgattctagccggcgtcgctagcgtaggtatcggaggcgctggccgacttcttgtcgcccgtcaagccaaagtccgtccagaacgttgcactgtgggacacgtgccgccctctacgtAcuIAsiSIBfuAIBspMIRsrIIBstAPIHgR1,4201,4401,4601,4801,5001,5201,5401,560
    ataggtcaggctctcgctgaactccccaatgtcaagcacttccggaatcgggagcgcggccgatgcaaagtgccgataaacataacgatctttgtagaaaccatcggcgcagctatttacccgcaggacatatccacgccctcctacatcgaagctgaaagcacgagattcttcgtatccagtccgagagcgacttgaggggttacagttcgtgaaggccttagccctcgcgccggctacgtttcacggctatttgtattgctagaaacatctttggtagccgcgtcgataaatgggcgtcctgtataggtgcgggaggatgtagcttcgactttcgtgctctaagaagcHgR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ccctccgagagctgcatcaggtcggagacgctgtcgaacttttcgatcagaaacttctcgacagacgtcgcggtgagttcaggctttttcatagggatcagcttgggctgtcctctccaaatgaaatgaacttccttatatagaggaagggtcttgcgaaggatagtgggattgtgggaggctctcgacgtagtccagcctctgcgacagcttgaaaagctagtctttgaagagctgtctgcagcgccactcaagtccgaaaaagtatccctagtcgaacccgacaggagaggtttactttacttgaaggaatatatctccttcccagaacgcttcctatcaccctaacaZraIAatIIBsaHIBsmBIEsp3IPflFIPshAITth111IHgR35S1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gcgtcatcccttacgtcagtggagatgtcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaatgatagcctttcctttatcgcaatgatggcatttgtcgcagtagggaatgcagtcacctctacagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctacgaggagcacccacccccaggtagaaaccctggtgacagccgtctccgtagaacttactatcggaaaggaaatagcgttactaccgtaaacaXmnIBmgBIBseRICspCIPshAI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aggagccaccttccttttctactgtcctttcgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgaaattaccctttgttgaaaagtctcaatagccctttggtcttctgagactgtatctttgacatttttggagtaggggtacgataacattaacgtttatcctcggtggaaggaaaagatgacaggaaagctacttcactgtctatcgacccgttaccttaggctcctccaaagggctttaatgggaaacaacttttcagagttatcgggaaaccagaagactctgacatagaaactgtaaaaacctcatccccatgctattgtaattgcaaatAclIBseRI2,1202,1402,1602,1802,2002,2202,2402,260
    caatttcgcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtagttaaagcgcggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacatBglIBmrIFspIPvuIIM13F2,2802,3002,3202,3402,3602,3802,4002,4202,440
    atacgactcactatagggcgaattgggtaccGGTCTCAccctTCGACGTAAAGCCTGTAGAAGAGGTTTCTAGCGAACGACACGAGTTTGAGCCTCATGAAGCTTCGTTGAACAACGGAAACTCGACTTGCCTTCCGCACAATACATCATTTCTTCTTAGCTTTTTTTCTTCTTCtatgctgagtgatatcccgcttaacccatggCCAGAGTgggaAGCTGCATTTCGGACATCTTCTCCAAAGATCGCTTGCTGTGCTCAAACTCGGAGTACTTCGAAGCAACTTGTTGCCTTTGAGCTGAACGGAAGGCGTGTTATGTAGTAAAGAAGAATCGAAAAAAAGAAGAAGAgeIBsaIKpnIAcc65IHindIIIUBQ6 promoter paperj5_0363_... AnnealPr...r2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTCGTTCATACAGTTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCAAAGCAAGTATGTCAAAAAAAAACAAATAGTCGAATGTAAAAGAACTTGGCATCGAAAGCAAAAGAAGAAAAATTGAAAGGTAAGCCTCAAAAACATAGAACAAAGTATCAAACAGGGTCCTAATCTTACTAATCCGTAGCTTGGAAGTTCTTAAACTAACTTATTTTGTAGAAGTUBQ6 promoter paper2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TTCTTAAGATATGAAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAAGAATTCTATACTTCTATTAGAAGTTTTCCGGGGACCCTTAGACTTTCTTCTCTTCGTCCGGGTAAATATACCCTTTCTTGTTATCATAAAGAATATATCCGGGTAAATTCAACTTTTGTTAGAAGTTTTCAGGGTGTAGCGAATCTATTCTTTTGCTTCGACTCAAATATATGMslIPasIAflIIBstXIBtgZIEcoO109IUBQ6 promoter paper2,8202,8402,8602,8802,9002,9202,9402,960
    AGCTAGAGTCGAAGTAGTGATTCATCACACACACTATTATGTgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttTTTGCAAAATTTTCCAGATCGATTTCTTCTTCCTCTGTTCTTCGGCGTTCAATTCGATCTCAGCTTCATCACTAAGTAGTGTGTGTGATAATACAcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaAAACGTTTTAAAAGGTCTAGCTAAAGAAGAAGGAGACAAGAAGCCGCAAGTTAClaIMslIBspDIgRNA HandleUBQ6 terminator paperUBQ6 pro...r paperpPIN1_Target1 2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TTCTGGGTTTTTCTCTTCGTTTTCTGTAACTGAAACCTAAAATTTGACCTAAAAAAAATCTCAAATAATATGATTCAGTGGTTTTGTACTTTTCAGTTAGTTGAGTTTTGCAGTTCCGATGAGATAAACCAATAACTTTGCTTAGATCTAATTCATTCCGTTACACCTCTGATGGAAGACCCAAAAAGAGAAGCAAAAGACATTGACTTTGGATTTTAAACTGGATTTTTTTTAGAGTTTATTATACTAAGTCACCAAAACATGAAAAGTCAATCAACTCAAAACGTCAAGGCTACTCTATTTGGTTATTGAAACGAATCTAGATTAAGTAAGGCAATGTGGAGACTACCUBQ6 terminator paper3,1603,1803,2003,2203,2403,2603,2803,3003,320
    AGATGGAAGGTTCTTAATAATGATGCCATTTTTTGGGTAATAATTTTGAATTAGAATCAAGGGTATAAGATTCATAATTAACATCACTTAAGCAAAGTTCGTAATATACGACCACAGGATATAATTTTTGGTACgagaTGAGACCgagctccagcttttgttccctttagtgaggTCTACCTTCCAAGAATTATTACTACGGTAAAAAACCCATTATTAAAACTTAATCTTAGTTCCCATATTCTAAGTATTAATTGTAGTGAATTCGTTTCAAGCATTATATGCTGGTGTCCTATATTAAAAACCATGctctACTCTGGctcgaggtcgaaaacaagggaaatcactccBsaISacIAflIIBanIIPflMIEco53kIUBQ6 terminator paperj5_0362...AnnealPo...r3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gttaattccgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagdcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtccaattaaggctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttchgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagM13R3,5203,5403,5603,5803,6003,6203,6403,660
    gggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtSapIBspQIPvuII3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggggataacgcaggaaagaacatgaaggccttgacaggatatattggcgggtaaactaagtcgctgtatgtgtttgtttgagatctgatctcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacccctattgcgtcctttcttgtacttccggaactgtcctatataaccgcccatttgattcagcgacatacacaaacaaactctagactagagtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactStuIRB3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagcgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgBciVI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtAlwNI4,2204,2404,2604,2804,3004,3204,3404,360
    gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaagaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagccttcttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaAcuI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagtDraIDraI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    atctaaagtatatatgtgtaacattggtctagtgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggttagatttcatatatacacattgtaaccagatcactaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccaPflMI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtctagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagNsiI4,9204,9404,9604,9805,0005,0205,0405,060
    atcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaattagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattaBsmISspIAsiSIBsmBIEcoNIEsp3IBpu10Isplit KAN F5,0805,1005,1205,1405,1605,1805,2005,2205,240
    acctggaatgctgttttccctgggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacaacattggcaacgctacctttgccatgtttcagaaacaacttggaccttacgacaaaagggaccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgttgtaaccgttgcgatggaaacggtacaaagtctttgttgaBsmINsiIPasI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctggcgcatcgggcttcccatacaatcggtagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggccttgagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgaccgcgtagcccgaagggtatgttagccatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggaactcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaataNruIBanII5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttccattcgtctgtcaaaataacaagtactactatataaaaatagaacacgttacattgtagtctctaaaactctgtgttgcaccgaaacaacttatttagcttgaaaacgactcaacttcctagtctagtgcgtagaagggctgttgcgtctggcaaggcaccgtttcgttttcaag5,6205,6405,6605,6805,7005,7205,7405,760
    aaaatcaccaactggtccacctacaacaaagctctcatcaaccgtggctccctcactttctggctggatgatggggcgattcaggcgatccccatccaacagcccgccgtcgagcgggcttttttatccccggaagcctgtggatagagggtagttatccacgtgaaaccgctaattttagtggttgaccaggtggatgttgtttcgagagtagttggcaccgagggagtgaaagaccgacctactaccccgctaagtccgctaggggtaggttgtcgggcggcagctcgcccgaaaaaataggggccttcggacacctatctcccatcaataggtgcactttggcgattBsaBI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgccccgcaaagccttgattcacggggctttccggcccgctccaaaaactatccacgtgaaatcgctaatcagggtacgtgaaatcgacggggcgtttcggaactaagtgccccgaaaggccgggcgaggtttttgataggtgcactttagcgattagtcccatgcactttagc5,9605,9806,0006,020
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    6037 bp45005000550050010001500HgRpUBQ6-pPIN1_Target1-r (DNA)pPIN1_Target1-f (DNA)pPIN1_Target1-gRNA_Handle-f (DNA)

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    6037