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pPbcsx28
  • Sequence Map
    Editing disabled on read-only sequence.
    caaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagaAcuIBceAIpUC19pMB1 origin of replicationRNAII transcript (complementary strand)RNAI transcript20406080100120140160
    gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcMmeIAlwNIBseYIpUC19pMB1 origin of replicationRNAII transcript (complementary strand)180200220240260280300320340
    ctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcaDrdIMmeIBciVIpUC19pMB1 origin of replicationRNAII transcript (complementary strand)360380400420440460480500520
    caggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcggtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcPciIAflIIIpUC19pMB1 ori...icationRNAII tr...strand)540560580600620640660680700
    cagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttagtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatSapIBsaXIBspQIPvuIICAP...tepUC19720740760780800820840860
    cactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcgactctagaggatccTTTACACTTTATGCTTCCGGCTCGTATGTTAGGAGGTCTTTATCatggattagtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgaacgtacggacgtccagctgagatctcctaggAAATGTGAAATACGAAGGCCGAGCATACAATCCTCCAGAAATAGtacctaatAccIPstISalISbfISphIXbaIBamHIBfuAIBspMIHincIIHindIIIMD2YLac promoterlac rep...g sitepUC198809009209409609801,0001,0201,040
    tatggaattagcgaaagaggcattctctataatatgtaccttcatagcagcatacgtggcctattactatgctatcaagcaactacaccaaaaaagtgttgagaatattgaatatgccaaataccaagctgtgttacaagcacataaaagcctgtataaactactcagatttactataccttaatcgctttctccgtaagagatattatacatggaagtatcgtcgtatgcaccggataatgatacgatagttcgttgatgtggttttttcacaactcttataacttatacggtttatggttcgacacaatgttcgtgtattttcggacatatttgatgagtctaaatgaBplIBplIBsmIBsaAIM4EL6AK8EA10FS12II14CT16FI18AA20YV22AY24YY26AI28KQ30LH32QK34SV36EN38IE40YA42KY44QA46VL48QA50HK52SL54YK56LL58RF60T1043-1576pbcsx28pUC191,0601,0801,1001,1201,1401,1601,1801,2001,220
    acaaatactgaaaatgaagacagcatattgatttgggaaaaaacaaaagatggtaagcaagaagcaacctattattttagaaaagaaaacatacgcaaatttataaaagagctttccaaagaaatatataacgaaggatgtggtatttttatgtcaaaagaggctctgtcactaatgtttatgacttttacttctgtcgtataactaaacccttttttgttttctaccattcgttcttcgttggataataaaatcttttcttttgtatgcgtttaaatattttctcgaaaggtttctttatatattgcttcctacaccataaaaatacagttttctccgagacagtgattApoIBbsIPsiIT62NT64EN66ED68SI70LI72WE74KT76KD78GK80QE82AT84YY86FR88KE90NI92RK94FI96KE98LS100KE102IY104NE106GC108GI110FM112SK114EA116LS118L1043-1576pbcsx28pUC191,2401,2601,2801,3001,3201,3401,3601,3801,400
    tttccgaatatagaaatattgtttatggtttcatgctttccgcccaaaacaatccccaagaaacaataagaataactaatagagagtctgtagaacgaatgaaaaagattcaccaaaacctctcgatagaaatcagacaagcaatcaatctgaaaaaacgagatttaagattttaaaaggcttatatctttataacaaataccaaagtacgaaaggcgggttttgttaggggttctttgttattcttattgattatctctcagacatcttgcttactttttctaagtggttttggagagctatctttagtctgttcgttagttagacttttttgctctaaattctaaaatBsaBII120SE122YR124NI126VY128GF130ML132SA134QN136NP138QE140TI142RI144TN146RE148SV150ER152MK154KI156HQ158NL160SI162EI164RQ166AI168NL170KK172RD174LR176F*1781043-1576pbcsx28pUC191,4201,4401,4601,4801,5001,5201,5401,560
    accgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgatggcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgctApoIBglIBmrIFspIKpnIPvuISacIBanIIBceAIEcoRIPvuIIAcc65IEco53kIpUC191,5801,6001,6201,6401,6601,6801,7001,7201,740
    atggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagataccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctDrdIKasINarINdeISfoITatIPluTIBstAPIpUC191,7601,7801,8001,8201,8401,8601,8801,9001,920
    caagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctagttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggatPfoIZraIAatIIBsmBIBsmBIEsp3IEsp3IEcoO109IpUC19pUC191,9401,9601,9802,0002,0202,0402,0602,0802,100
    tttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctaaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaBciVIblablapUC192,1202,1402,1602,1802,2002,2202,2402,260
    ggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttAclIAcuIXmnIblapUC192,2802,3002,3202,3402,3602,3802,4002,4202,440
    gagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttccBcgIPvuIScaITatIAvaIIblaNew AGAAW target 2pUC192,4602,4802,5002,5202,5402,5602,5802,6002,620
    agctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcatcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgtAclIFspIBsrDIblapUC192,6402,6602,6802,7002,7202,7402,7602,7802,800
    acaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgtgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgcBglIBmrIBpmIBsaIAvaIIBsrDIBsrFINmeAIIIblapUC192,8202,8402,8602,8802,9002,9202,9402,960
    acggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtAhdIblapUC192,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaaactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttpUC19pMB1 origin of replication3,1603,1803,2003,2203,2403,260
    • Plasmid
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    Editing disabled on read-only sequence.
    3264 bp2250250027503000250500750pUC19blaNew AGAAW target 2RNAI promoter (clockwise); TTGAAGpMB1 origin of replication

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    3264