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  • 0 feature results
pKR338 = pRS416-dCas9-Mxi1-pRPR1(TetO)-gRNA + TetR + HphMX
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCCTAGCGAACGGACATTGAATGTGCGCGGAGCATAGAAAATTACTACCTTATTAAACCCTTAAATGAGACACAAATAAATAAAAATACAAAACATAAACCTAAAATCCEN/ARS20406080100120140160
    AAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTTTCATTTATTTCTTCCATCTTCTCAATGCCTTACTTCTTTTTTTTTATTTGTTTCCAAATTTTTTAAAGTTGTTTTTCGCATGAAATGTATATATAAATAATCTGTTCTTTTCGTCTAATTTATCTATATGTAAGCTAATTGCTATTCATTTTACATTTTAGTGTCCTAAAAGCCEN/ARS180200220240260280300320340
    TGTGTGGTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAACACACCAGAAGATGTGTCTGTTCTACTTTGTTAAGCCGTAATTATGGACTCTCGTCCTTCTCGTTCTATTTTCCATCATAAACAACCGCTAGGGGGATCTCAGAAAATGTAGAAGCCTTTTGTTTTTGATAAAAAAGAAATTAAAGAAAAAAATGAAAGATAAAAATTAAATATCEN/ARS360380400420440460480500520
    TATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTATAAATATAATTTTTTAAATTTAATATTAATAAAAATATCGTGCACTACTTTTCCTGGGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAAPmlISwaIAmpR promoterCEN/ARS540560580600620640660680700
    GAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAAmpRAmpRAmpR...ter720740760780800820840860
    CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCAmpR8809009209409609801,0001,0201,040
    GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCACTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAmpR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATABglIAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTAhdIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTC1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGori2,1202,1402,1602,1802,2002,2202,2402,260
    ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTGGCCTTTTGCTCgggggatctgccaattgaacataacatggtagttacatatactagtaataTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGACCGGAAAACGAGccccctagacggttaacttgtattgtaccatcaatgtatatgatcattatMfeISpeIoripRPR1Pci1-UPra...n2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tggttcggcacacattaaaagtataaaaactatctgaattacgaattacatatattggtcataaaaatcaatcaatcatcgtgtgttttatatgtctcttatctaagtataagaatatccatagttaatattcacttacgctaccttttaacctgtaatcattgtcaacaggataaccaagccgtgtgtaattttcatatttttgatagacttaatgcttaatgtatataaccagtatttttagttagttagtagcacacaaaatatacagagaatagattcatattcttataggtatcaattataagtgaatgcgatggaaaattggacattagtaacagttgtcctatpRPR12,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgttaacgacccacattgataaacgctagtatttctttttcctcttcttattggccggctgtctctatactcccctatagtctgtttcttttcgtttcgattgtccctatcagtgatagagatggcgcacatggtacgctgtggtgctcgcggctgggaacgaaactctgggagcacaattgctgggtgtaactatttgcgatcataaagaaaaaggagaagaataaccggccgacagagatatgaggggatatcagacaaagaaaagcaaagctaacagggatagtcactatctctaccgcgtgtaccatgcgacaccacgagcgccgacccttgctttgagaccctcgHpaIpRPR1RNAse P leader sequenceTetO2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgcgattggcagCGGCCGCgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttctcgagccatatccaacttccaatttaatctttcttttttaattttcacttatttgcgatacagaaagaaaaaaacgctaaccgtcGCCGGCGcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaagagctcggtataggttgaaggttaaattagaaagaaaaaattaaaagtgaataaacgctatgtctttcttttttNotIsgRNARPR1tpRPR1RNA...ce2,8202,8402,8602,8802,9002,9202,9402,960
    gcgatagtaactattgaattttgtttggatttggttagattagatatggtttctctttatatttacatgctaaaaatgggctacaccagagatacataattagatatatatacgccagtacaccttatcggcccaagccttgtcccaaggcagcgttttgttcttggaaacgctgcgctatcattgataacttaaaacaaacctaaaccaatctaatctataccaaagagaaatataaatgtacgatttttacccgatgtggtctctatgtattaatctatatatatgcggtcatgtggaatagccgggttcggaacagggttccgtcgcaaaacaagaacctttgcgacRPR1t2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccctacacgttcgctatgcttcaagaacttttctgagcacttcatgatgcatgtttgttccttattggttagctttgatgttgtgaagtcattgacacagtctgtgaaacatctttctaccagattagagtacaaacgcatgaaatccttcatttgcttttgttccactacttttgggatgtgcaagcgatacgaagttcttgaaaagactcgtgaagtactacgtacaaacaaggaataaccaatcgaaactacaacacttcagtaactgtgtcagacactttgtagaaagatggtctaatctcatgtttgcgtactttaggaagtaaacgaaaacaaggtgatgaaaaRPR1t3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tggaactcttgttgttctttCCTGCAGGtagtcgtgcaatgtatgactttaagatttgtgagcaggaagaaaagggagaatcttctaacgataaacccttgaaaaactgggtagactacgctatgttgagttgctacgcaggctgcacaattacacgagaatgctcccgcctaggaccttgagaacaacaagaaaGGACGTCCatcagcacgttacatactgaaattctaaacactcgtccttcttttccctcttagaagattgctatttgggaactttttgacccatctgatgcgatacaactcaacgatgcgtccgacgtgttaatgtgctcttacgagggcggatccAvrIIpGPM1RPR1t3,3403,3603,3803,4003,4203,4403,4603,4803,500
    atttaaggctaagggacgtgcaatgcagacgacagatctaaatgaccgtgtcggtgaagtgttcgccaaacttttcggttaacacatgcagtgatgcacgcgcgatggtgctaagttacatatatatatatatatatatatatatatatatatagccatagtgatgtctaagtaataaattccgattccctgcacgttacgtctgctgtctagatttactggcacagccacttcacaagcggtttgaaaagccaattgtgtacgtcactacgtgcgcgctaccacgattcaatgtatatatatatatatatatatatatatatatatatcggtatcactacagattcattHpaIpGPM13,5203,5403,5603,5803,6003,6203,6403,660
    cctttatggtatatttcttaatgtggaaagatactagcgcgcgcacccacacacaagcttcgtcttttcttgaagaaaagaggaagctcgctaaatgggattccactttccgttccctgccagctgatggaaaaaggttagtggaacgatgaagaataaaaagagagatccactgggaaataccatataaagaattacacctttctatgatcgcgcgcgtgggtgtgtgttcgaagcagaaaagaacttcttttctccttcgagcgatttaccctaaggtgaaaggcaagggacggtcgactacctttttccaatcaccttgctacttcttatttttctctctaggtgacpGPM13,6803,7003,7203,7403,7603,7803,8003,8203,840
    aggtgaaatttcagctgacagcgagtttcatgatcgtgatgaacaatggtaacgagttgtggctgttgccagggagggtggttctcaacttttaatgtatggccaaatcgctacttgggtttgttatataacaaagaagaaataatgaactgattctcttcctccttcttgtccttccactttaaagtcgactgtcgctcaaagtactagcactacttgttaccattgctcaacaccgacaacggtccctcccaccaagagttgaaaattacataccggtttagcgatgaacccaaacaatatattgtttcttctttattacttgactaagagaaggaggaagaacaggaAleIpGPM1497_GPM1_FwdSeqPrime3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttcttaattctgttgtaattaccttcctttgtaattttttttgtaattattcttcttaataatccaaacaaacacacatattacaatatattaaaatgtctagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacaagaattaagacaacattaatggaaggaaacattaaaaaaaacattaataagaagaattattaggtttgtttgtgtgtataatgttatataattttacagatctaatctattttcatttcactaattgtcgcgtaatctcgacgaattactccagccttagcttccaaattgttgBarIpGPM1TetR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagtggcatttgagcgggtcttcgatccacatctcgtcggatgtaacataaccgtacattttttattcgcccgaaacgagctgcggaatcggtaactctacaatctatccgtggtatgagtgaaaacgggaaatcttcccctttcgaccgttctaaaaaatgcattattgcgattttcaSnaBITetR4,2204,2404,2604,2804,3004,3204,3404,360
    tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttaaatctacacgaaatgattcagtagcgctacctcgttttcatgtaaatccatgtgccggatgtctttttgtcatactttgagagcttttagttaatcggaaaaatacggttgttccaaaaagtgatctcttacgtaatatacgtgagtcgcgacaccccgtaaaatgaaatccaaNruITetR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaacgcataaccttctagttctcgtagttcagcgatttcttctttccctttgtggatgatgactatcatacggcggtaataatgctgttcgatagcttaataaactagtggttccacgtctcggtcggaagaataagccggaacttaactagtatacgcctaatctttttgttgaattAleITetR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    atgtgaaagtgggtcttaagtctgaagaatgaatgatttgatgatttctttttccctccatttttcttactgaatatatcaatgatatagacttgtatagtttattatttcaaattaagtagctatatatagtcaagataacgtttgtttgacacgattacattattcgtcgacatacactttcacccagaattcagacttcttacttactaaactactaaagaaaaagggaggtaaaaagaatgacttatatagttactatatctgaacatatcaaataataaagtttaattcatcgatatatatcagttctattgcaaacaaactgtgctaatgtaataagcagctgtSalItGPM1498_GPM1t_RevSeqPrimeTetR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tcttttttcagcctgtcgtggtagcaatttgaggagtattattaattgaataggttcattttgcgctcgcataaacagttttcgtcagggacagtatgttggaatgagtggtaattaatggtgacatgacatgttatagcaataaccttgatgtttacatcgtagtttaatgtacagaaaaaagtcggacagcaccatcgttaaactcctcataataattaacttatccaagtaaaacgcgagcgtatttgtcaaaagcagtccctgtcatacaaccttactcaccattaattaccactgtactgtacaatatcgttattggaactacaaatgtagcatcaaattacatgBsrGItGPM14,9204,9404,9604,9805,0005,0205,0405,060
    accccgcgaattcgttcaagtaggagtgcaccaattgcaaagggaaaagctgaatgggcagttcgaaGACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCAtggggcgcttaagcaagttcatcctcacgtggttaacgtttcccttttcgacttacccgtcaagcttCTGTACCTCCGGGTCTTATGGGAGGAACTGTCAGAACTGCACGCGTCGAGTCCCCGTACTACACTGACAGCGGGCATGTAAATCGGGTATGTAGGGGTACATATTAGTMfeITEF promoterHphMX DNAtGPM1211_tGPM1_RevPrime5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCGGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTCGCCACTGAGGTTCTTCTTTCATAAAACGTAGGTATGTAAAACTACCGGCGTGCCGCGCTTCGTTTTTAATGCCGAGGAGCGACGCCTGGACGCTCGTCCCTTTGCGAGGGGAGTGTCTGCGCAACTTAACAGGGGTGCGGCGCGGGGACATCTCTTTATATTTTCCAATCCTAAGCGGTGACTCCAAGAAGAAAGTATMluITEF promoterHphMX DNA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    TACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACCATGGGTAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCATGAAGGAAAATTTTAGAACGATCCTATGTCAAGAGTGTAGTGTAGGCTTGTATTTGTTGGTACCCATTTTTCGGACTTGAGTGGCGCTGCAGACAGCTCTTCAAAGACTAGCTTTTCAAGCTGTCGCAGAGGCTGGACTACGTCGAGAGCCTCCCGCTTCTTAGAGCACGAAAGZraIAatIIPshAIHphMX DNAHphMXTEF promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    AGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACTCGAAGCTACATCCTCCCGCACCTATACAGGACGCCCATTTATCGACGCGGCTACCAAAGATGTTTCTAGCAATACAAATAGCCGTGAAACGTAGCCGGCGCGAGGGCTAAGGCCTTCACGAACTGTAACCCCTTAAGTCGCTCTCGGACTGGATAACGTAGAGGGCGGCACGTGHphMX DNAHphMX5,6205,6405,6605,6805,7005,7205,7405,760
    AGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTCCCACAGTGCAACGTTCTGGACGGACTTTGGCTTGACGGGCGACAAGACGTCGGCCAGCGCCTCCGGTACCTACGCTAGCGACGCCGGCTAGAATCGGTCTGCTCGCCCAAGCCGGGTAAGCCTGGCGTTCCTTAGCCAGTTATGTGATGTACCGCACTAAAGTATACGCGCTAAsiSIRsrIIHphMX DNAHphMX5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAACGACTAGGGGTACACATAGTGACCGTTTGACACTACCTGCTGTGGCAGTCACGCAGGCAGCGCGTCCGAGAGCTACTCGACTACGAAACCCGGCTCCTGACGGGGCTTCAGGCCGTGGAGCACGTGCGCCTAAAGCCGAGGTTGTTACAGGACTGCCTGTTACCGGCGTATTGTHphMX DNAHphMX5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCCGCCAGTAACTGACCTCGCTCCGCTACAAGCCCCTAAGGGTTATGCTCCAGCGGTTGTAGAAGAAGACCTCCGGCACCAACCGAACATACCTCGTCGTCTGCGCGATGAAGCTCGCCTCCGTAGGCCTCGAACGTCCTAGCGGCGCCGAGGCCCGCATATACGAGGCGTAACCAGAjuISacIIHphMX DNAHphMX6,1406,1606,1806,2006,2206,2406,2606,2806,300
    TTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAACTGGTTGAGATAGTCTCGAACCAACTGCCGTTAAAGCTACTACGTCGAACCCGCGTCCCAGCTACGCTGCGTTAGCAGGCTAGGCCTCGGCCCTGACAGCCCGCATGTGTTTAGCGGGCGTCTTCGCGCCGGCAGACCTGGCTACCGACACATCTTCATGAGCGGCTATCACCHphMX DNAHphMX6,3206,3406,3606,3806,4006,4206,4406,460
    AAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAATCAGTACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGTTTGGCTGCGGGGTCGTGAGCAGGCTCCCGTTTCCTTATTAGTCATGACTGTTATTTTTCTAAGAACAAAAGTTCTTGAACAGTAAACATATCAAAAAAATATAACATCAACAAGATAAAATTAGTTTACAATCGCACTAAATATAAAAAAAAGCGGAGCTGTAGTAGACGGGTCHphMX DNATEF terminatorHphMX6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGTTGTGCATACACTACTAGGGAAGACTTGAAAAAAACCTAGGAAATGAAAAAACGACACAGGAAGTCCCGTATTTACTATTCTTTCCTGCGTTATCCCCTGATTCTGTTACGCTTCAATTCACGCGTCTTTCATTATAGTACGCAGTTAGCATACACTTACGACCAGCGATATGACAACACGTATGTGATGATCCCTTCTGAACTTTTTTTGGATCCTTTACTTTTTTGCTGTGTCCTTCAGGGCATAAATGATAAGAAAGGACGCAATAGGGGACTAAGACAAvrIIHphMX DNATEF terminatorInserted_Seq6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTT6,8406,8606,8806,9006,9206,9406,9606,9807,000
    GCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTACCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCCTATGTTGTGTGGAATTGTGAGCGGATAACAATTtcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaaCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATGGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGGATACAACACACCTTAACACTCGCCTATTGTTAAagtgtgtcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttM13-revM13_re...rimerlac promoterlac operatorT3_promoterT37,0207,0407,0607,0807,1007,1207,1407,160
    gggaacaaaagctggAGCTCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGcccttgttttcgaccTCGAGTATCGAAGTTTTACAAAGATGAGGAAAAAATGAGAAGGTCTAAAAGAGCCTGAGGCGCGTAGCGGCATGGTGAAGTTTTGTGGGTTCGTGTCGTATGATTTAAAGGGGAGAAAGAAGGAGATCCCACAGCAATTAATGGGCATGATTTCCAAACCTEF PromoterM13_pUC_rev_primer7,1807,2007,2207,2407,2607,2807,3007,3207,340
    AAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTTTCTTTTTTCTCTGGCGGAGCAAAGAAAAAGAAGCAGCTTTTTCCGTTATTTTTAAAAATAGTGCAAAGAAAAAGAACTTTTAAAAAAAAAACTAAAAAAAGAGAAAGCTACTGGAGGGTAACTATAAATTCAATTATTTGCCAGAAGTTAAAGAGTTCAAAGTCAAAGTAAATEF Promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    TTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTCTAGAGatgtctagagccccaaagaagaagagaaaagttagacccgggATGGACAAGAAGTACTCCATTGGGCTCGCTATCGGCACAAACAGCGTCGGCTGGGAAGAACAAGATAATGTTGAAAAAAATGAAGAACGAGTAATCTTTCTTTCGTATCGTTAGATTAGATTCAAAAGATCTCtacagatctcggggtttcttcttctcttttcaatctgggcccTACCTGTTCTTCATGAGGTAACCCGAGCGATAGCCGTGTTTGTCGCAGCCGACCCSmaIXmaITspMIMD2KK4YS6IG8LA10IG12TN14SV16GW187646-11761M1S2R3A4P5K6K7K8R9K10V11R12P13G147604-7645TEF PromoterdCas9(Church)dCas9_VP64mNLS7,5407,5607,5807,6007,6207,6407,6607,6807,700
    CCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGGGCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAAAGAAGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACCCGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTTTCTTKasINarISfoIPluTIAV20IT22DE24YK26VP28SK30KF32KV34LG36NT38DR40HS42IK44KN46LI48GA50LL52FD54SG56ET58AE60AT62RL64KR66TA68RR70RY72TR74RK76N7646-11761dCas9(Church)7,7207,7407,7607,7807,8007,8207,8407,860
    TCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATAAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGAGAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATXcmIR78IC80YL82QE84IF86SN88EM90AK92VD94DS96FF98HR100LE102ES104FL106VE108ED110KK112HE114RH116PI118FG120NI122VD124EV126AY128HE130KY132PT134I7646-11761dCas9(Church)7,8807,9007,9207,9407,9607,9808,0008,0208,040
    TATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCATAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAGAGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGSalIY136HL138RK140KL142VD144ST146DK148AD150LR152LI154YL156AL158AH160MI162KF164RG166HF168LI170EG172DL174NP176DN178SD180VD182KL184FI186QL188VQ190TY192N7646-11761dCas9(Church)8,0608,0808,1008,1208,1408,1608,1808,2008,220
    AGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTCGAAAAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCGACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAPasIQ194LF196EE198NP200IN202AS204GV206DA208KA210IL212SA214RL216SK218SR220RL222EN224LI226AQ228LP230GE232KK234NG236LF238GN240LI242AL244SL246GL248TP250NF2527646-11761dCas9(Church)8,2408,2608,2808,3008,3208,3408,3608,3808,400
    TAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGATTTAGATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGCTGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCBsaBIKS254NF256DL258AE260DA262KL264QL266SK268DT270YD272DD274LD276NL278LA280QI282GD284QY286AD288LF290LA292AK294NL296SD298AI300LL302SD304IL306RV308NT3107646-11761dCas9(Church)8,4208,4408,4608,4808,5008,5208,5408,560
    GAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCCTCTAGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACTTCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGBlpIEI312TK314AP316LS318AS320MI322KR324YD326EH328HQ330DL332TL334LK336AL338VR340QQ342LP344EK346YK348EI350FF352DQ354SK356NG358YA360GY362ID364GG366AS3687646-11761dCas9(Church)8,5808,6008,6208,6408,6608,6808,7008,7208,740
    AGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGCAAGATCCTCCTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTCTTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTBsu36IQE370EF372YK374FI376KP378IL380EK382MD384GT386EE388LL390VK392LN394RE396DL398LR400KQ402RT404FD406NG408SI410PH412QI414HL416GE418LH420AI422LR424RQ426E7646-11761dCas9(Church)8,7608,7808,8008,8208,8408,8608,8808,9008,920
    GGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCGCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAAGGGGCCTAAAGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATTCCCCSmaIXmaITspMID428FY430PF432LK434DN436RE438KI440EK442IL444TF446RI448PY450YV452GP454LA456RG458NS460RF462AW464MT466RK468SE470ET472IT474PW476NF478EE480VV482DK484G7646-11761dCas9(Church)8,9408,9608,9809,0009,0209,0409,0609,0809,100
    GCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGAGAGCCGGAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCTCTCGBarIBsaBIA486SA488QS490FI492ER494MT496NF498DK500NL502PN504EK506VL508PK510HS512LL514YE516YF518TV520YN522EL524TK526VK528YV530TE532GM534RK536PA538FL540SG542E7646-11761dCas9(Church)9,1209,1409,1609,1809,2009,2209,2409,260
    AGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCATTAATCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGTAATTPasIQ544KK546AI548VD550LL552FK554TN556RK558VT560VK562QL564KE566DY568FK570KI572EC574FD576SV578EI580SG582VE584DR586FN588AS590LG592TY594HD596LL598KI600IK6027646-11761dCas9(Church)9,2809,3009,3209,3409,3609,3809,4009,4209,440
    AGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGATTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATGGGGGTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTACCCCCKasINarISfoIPluTIDK604DF606LD608NE610EN612ED614IL616ED618IV620LT622LT624LF626ED628RE630MI632EE634RL636KT638YA640HL642FD644DK646VM648KQ650LK652RR654RY656TG658WG6607646-11761dCas9(Church)9,4609,4809,5009,5209,5409,5609,5809,6009,620
    CGGCTGTCAAGAAAACTGATCAATGGGATCCGAGACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACTTCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCAACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTAjuIBamHICspCIRL662SR664KL666IN668GI670RD672KQ674SG676KT678IL680DF682LK684SD686GF688AN690RN692FM694QL696IH698DD700SL702TF704KE706DI708QK710AQ712VS714GQ716GD7187646-11761dCas9(Church)9,6409,6609,6809,7009,7209,7409,7609,7809,800
    GTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAATGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCCCTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCGGGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCSL720HE722HI724AN726LA728GS730PA732IK734KG736IL738QT740VK742VV744DE746LV748KV750MG752RH754KP756EN758IV760IE762MA764RE766NQ768TT770QK772GQ774KN776S7646-11761dCas9(Church)9,8209,8409,8609,8809,9009,9209,9409,960
    TAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATGAGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGGACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGKflIR778ER780MK782RI784EE786GI788KE790LG792SQ794IL796KE798HP800VE802NT804QL806QN808EK810LY812LY814YL816QN818GR820DM822YV824DQ826EL828DI830NR832LS834D7646-11761dCas9(Church)9,98010,00010,02010,04010,06010,08010,10010,12010,140
    TACGACGTGGATGCTATCGTGCCCCAGTCTTTTCTCAAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAAATAGAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACATGCTGCACCTACGATAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTCACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGY836DV838DA840IV842PQ844SF846LK848DD850SI852DN854KV856LT858RS860DK862NR864GK866SD868NV870PS872EE874VV876KK878MK880NY882WR884QL886LN888AK890LI892T7646-11761dCas9(Church)10,16010,18010,20010,22010,24010,26010,28010,30010,320
    AACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTTGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGATTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTPmlIPsrIBstXIQ894RK896FD898NL900TK902AE904RG906GL908SE910LD912KA914GF916IK918RQ920LV922ET924RQ926IT928KH930VA932QI934LD936SR938MN940TK942YD944EN946DK948LI950RE9527646-11761dCas9(Church)10,34010,36010,38010,40010,42010,44010,46010,48010,500
    GGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATTACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACTCTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGFspAIVK954VI956TL958KS960KL962VS964DF966RK968DF970QF972YK974VR976EI978NN980YH982HA984HD986AY988LN990AV992VG994TA996LI998KK1000YP1002KL1004ES1006EF1008VY10107646-11761dCas9(Church)10,52010,54010,56010,58010,60010,62010,64010,660
    GGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCTTTTACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCATGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGD1012YK1014VY1016DV1018RK1020MI1022AK1024SE1026QE1028IG1030KA1032TA1034KY1036FF1038YS1040NI1042MN1044FF1046KT1048EI1050TL1052AN1054GE1056IR1058KR1060PL1062IE1064TN1066GE10687646-11761dCas9(Church)10,68010,70010,72010,74010,76010,78010,80010,82010,840
    CAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTAAAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGAGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAATTTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTKflINruIPaqCITG1070EI1072VW1074DK1076GR1078DF1080AT1082VR1084KV1086LS1088MP1090QV1092NI1094VK1096KT1098EV1100QT1102GG1104FS1106KE1108SI1110LP1112KR1114NS1116DK1118LI1120AR1122KK1124DW1126D7646-11761dCas9(Church)10,86010,88010,90010,92010,94010,96010,98011,00011,020
    CCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAAAAACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTTTTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTP1128KK1130YG1132GF1134DS1136PT1138VA1140YS1142VL1144VV1146AK1148VE1150KG1152KS1154KK1156LK1158SV1160KE1162LL1164GI1166TI1168ME1170RS1172SF1174EK1176NP1178ID1180FL1182EA1184K7646-11761dCas9(Church)11,04011,06011,08011,10011,12011,14011,16011,18011,200
    GGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAACCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGATCACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTG1186YK1188EV1190KK1192DL1194II1196KL1198PK1200YS1202LF1204EL1206EN1208GR1210KR1212ML1214AS1216AG1218EL1220QK1222GN1224EL1226AL1228PS1230KY1232VN1234FL1236YL1238AS1240HY1242E7646-11761dCas9(Church)11,22011,24011,26011,28011,30011,32011,34011,360
    AGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAATAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTAAGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAK1244LK1246GS1248PE1250DN1252EQ1254KQ1256LF1258VE1260QH1262KH1264YL1266DE1268II1270EQ1272IS1274EF1276SK1278RV1280IL1282AD1284AN1286LD1288KV1290LS1292AY1294NK1296HR1298DK1300PI13027646-11761dCas9(Church)11,38011,40011,42011,44011,46011,48011,50011,52011,540
    CAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCATAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCATGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGAscIRE1304QA1306EN1308II1310HL1312FT1314LT1316NL1318GA1320PA1322AF1324KY1326FD1328TT1330ID1332RK1334RY1336TS1338TK1340EV1342LD1344AT1346LI1348HQ1350SI1352TG1354LY1356ET1358RI13607646-11761dCas9(Church)11,56011,58011,60011,62011,64011,66011,68011,70011,720
    GACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACGAGGGAGCTCCCAAGAAAAAGCGCAAGGTAGGTAGTTCCAAGCTTGGCGGCAGCGGCGGCAGCATGGAACGTGTGAGAATGATTAATGTGCAAAGGCTGTTAGAAGCCGCAGAGTTTTTAGAAAGAAGAGAAAGAGAATCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGCTCCCTCGAGGGTTCTTTTTCGCGTTCCATCCATCAAGGTTCGAACCGCCGTCGCCGCCGTCGTACCTTGCACACTCTTACTAATTACACGTTTCCGACAATCTTCGGCGTCTCAAAAATCTTTCTTCTCTTTCTCTTADL1362SQ1364LG1366GD1368SR1370AD13727646-11761E1G2A3P4K5K6K7R8K9V10G11S12S13K14L15G16G17S18G19G20S21M22E23R24V25R26M27I28N29V30Q31R32L33L34E35A36A37E38F39L40E41R42R43E44R45E4611762-11953MxiI DomaindCas9(Church)NLS11,74011,76011,78011,80011,82011,84011,86011,88011,900
    GCGAACACGGGTATGCCAGTTCTTTCCCTAGCATGCCCTCTCCCAGAGGCTAACTCGAGCTCTTCTCGAGTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTCGCTTGTGCCCATACGGTCAAGAAAGGGATCGTACGGGAGAGGGTCTCCGATTGAGCTCGAGAAGAGCTCAGTACATTAATCAATACAGTGCGAATGTAAGTGCGGGAGGGGGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAASphIPspXIC47E48H49G50Y51A52S53S54F55P56S57M58P59S60P61R62G63*6411762-11953CYC1 TerminatorMxiI Domain11,92011,94011,96011,98012,00012,02012,04012,060
    TTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGGTACccaattcgccctatagtgagtcgAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTTAAAAAGAAAAAAAAGACATGTCTGCGCACATGCGTACATTGTAATATGACTTTTGGAACGAACTCTTCCAAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCCATGggttaagcgggatatcactcagcKpnIMluIBsrGIAcc65ICYC1 TerminatorT7T7_p...ter12,08012,10012,12012,14012,16012,18012,20012,22012,240
    tattacgcgcgctcactggccgtcgttttacaACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGataatgcgcgcgagtgaccggcagcaaaatgtTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCBglIlacZαM13-fwdM13_f...imerlacZ_a12,26012,28012,30012,32012,34012,36012,38012,40012,420
    ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGClacZαf1 ori12,44012,46012,48012,50012,52012,54012,56012,58012,600
    ATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAloIf1 orilacZα12,62012,64012,66012,68012,70012,72012,74012,760
    TTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATCCCATTATTGACTATATf1 ori12,78012,80012,82012,84012,86012,88012,90012,92012,940
    ATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCCTGCTTTTCTGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATTAATTTAACTTCGAGATTAAACACTCAAATCATATGTACGTAAATGAATATTATGTCAAAAAATCAAAACGACCGGCGTAGAAGAGTTTATACGAAGGGTCGGACGAAAAGACATTGCAAGTGGGAGATGGAATCGTAGGGAAGGGAAACGTTTATCAGGAGAAGGTTGTTATTABstZ17I*268NQ266QG264CR262RL260YA258EW256GA254KR252YR250EG248EV246KA244DR242GK240AF238LG236RG234VI232I13012-13815URA312,96012,98013,00013,02013,04013,06013,08013,10013,120
    AATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGGTGTCATAATCAACCAATCGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCTTACAGTCTAGGACATCTCTGGTGTAGTAGGTGCCAAGATATGACAACTGGGTTACGCAGAGGGAACAGTAGATTTGGGTGTGGCCCACAGTATTAGTTGGTTAGCATTGGAAGTAGAGAAGGTGGGTACAGAGAAACTCGTTATTTCGGCTATTGTTTTAGAAACAGCGAGAAGI230DS228GT226SV224VD222DV220TR218YQ216QG214LA212DG210KD208DL206GV204GP202TM200IL198WD196YG194ED192RG190GM188DR186QA184IF182GI180VF178DK176DS174K13012-13815URA313,14013,16013,18013,20013,22013,24013,26013,28013,300
    GCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGCCTCTAGGTTCCTTTGTTACTTCTTCTGCCGCCTGCTTCAAACCGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCGTTACAGTTGTCATGGGAATCATATAAGAGGTCATCTATCCCTCGGGAACGTACTGTTAAGACGATTGTAGTTTTCCGGAGATCCAAGGAAACAATGAAGAAGACGGCGGACGAAGTTTGGCGATTGTTATGGACCCGGGTGGTGTGGCACACGTAAGCATTACAGACGGGTAAApaIStuIPspOMIA172ID170VT168GK166TY164EG162TS160LS158GK156CS154LE152AL150ML148LG146RP144EK142TV140EE138AA136QK134LG132SV130IG128PG126VV124GH122AN120TI118DA116WE11413012-13815URA313,32013,34013,36013,38013,40013,42013,44013,460
    CTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAAATTGTACTTGGCGGATAATGCCTTTAGCGGCTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAGACGATAAGACATATGTGGGCGTCTCATGACGTTAAACTGACATAATGGTTACAGTCGTTTAAAAGACAGAAGCTTCTCATTTTTTAACATGAACCGCCTATTACGGAAATCGCCGAATTGACACGGGAGGTACCTTTTTAGTCAGTTCTATAGGTGTACACAAAAATCATTTGTEcoRVBstZ17IAI112RY110VG108AS106YQ104LK102VT100NG98ID96AF94KR92DE90FL88LF86NY84KA82SL80AK78LP76KV74TG72EM70SF68DT66LI64DV62HT60KL58LC5613012-13815URA313,48013,50013,52013,54013,56013,58013,60013,62013,640
    AATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTGTTTGCTTTTCGTGCATGATATTAAATAGCTTGGCAGCAACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTTTAAAACCCTGGATTACGAAGTTGATTGAGGTCATTAAGGAACCACCATGCTTGTAGGTTACTTCGTGTGTTCAAACAAACGAAAAGCACGTACTATAATTTATCGAACCGTCGTTGTCCTGATCCTACTCATCGTCGTGCAAGGAATATACATCGAAAGCTGTACTAAATAGAAXcmIIK54PG52LA50EV48LE46LL44EK42TT40RV38DL36SA34CL32NT30QK28EH26MI24NF22LK20AA18VP16SP14HT12AA10RE8KY6TA4KS2M13012-13815URA3UR...r13,66013,68013,70013,72013,74013,76013,78013,80013,820
    CGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATATGCGTATATATACCAATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAGCAAAGGACGTCCAAAAACAAGACACGTCAACCCAATTCTTATGACCCGTTAAAGTACAAAGAAGTTGTGATGTATACGCATATATATGGTTAGATTCAGACACGAGGAAGGAAGCAAGAAGGAAGACAAGCCTCTAATGGCTTAGTTTTTTTAAAGTTTCTTTGGCTTTAGTTTURA3 promoter13,84013,86013,88013,90013,92013,94013,96013,98014,000
    AAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACTTTTTCTTATTTTTTTTTTACTACTTAACTTAACTTTTCGACACCATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGURA3 promoter14,02014,04014,06014,08014,10014,12014,14014,160
    CGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT14,18014,20014,220
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    14231 bp10000110001200013000100020003000dCas9(Church)pGPM1TetOlacZ_af1 ori7646-1176113012-1381511762-1195351-pRPR1-NotI-sgRNA-F (DNA)195-gRNA_delete_rev (DNA)37 SacII-gRNA-oligo (DNA)ODM002 (DNA)ODM001 (DNA)gRNA_ds_primer (DNA)73-gRNA-5'Rev+4bp (DNA)q29-gRNA-L (DNA)150-gRNA-outer-3'extender (DNA)JS244-Pci1-RPR1t-Rev (DNA)50-RPR1_term_rev (DNA)53-pGPM1_prom_fwd (DNA)124-GPM1-tHg-Rev (DNA)136-site18up-GPM1-fwd (DNA)56-tGPM1_term_rev (DNA)133-Tef1-HphMX-Fwd (DNA)JS374-T3-Adj-R (DNA)p414_Cas9_rev (DNA)TEF1_rev (DNA)43-TEF1-5'-NLS-oligo (DNA)44-NLS-dCas9(Lu)-fwd (DNA)42-5'-NLS-dCas9-fwd (DNA)Gal1_Cas9_Fwd (DNA)Met3_Cas9_Fwd (DNA)Cas9_H840A_rev (DNA)p414_Cas9_PacI_fwd (DNA)CYC1_fwd (DNA)66-CYC1t-5'-Fwd (DNA)198 second half (DNA)198_second_half (DNA)ODM019 (DNA)203 (DNA)204 (DNA)CEN/ARS,JS727-B15-BspQI_removal_CEN/ARS (DNA)SwaIPmlIAmpR promoter,AmpRAmpR180-Mid-Amp-Rev2 (DNA),mid_Amp_fwd (DNA),+2AhdIoriJS225-ori-fwd (DNA)JS213-Pci1-UP-Extender (DNA),+1random insersionPci1-UP,110-Pci1-UP-Rev (DNA),+8pRPR1192-Mid-pRPR1-fwd (DNA),HpaITSS,RNAse P leader sequence,+6sgRNA,+10196-gRNA_delete_fwd (DNA),+2130-RPR1t-int-rev (DNA),+274-RPR1t-5'-Rev (DNA)RPR1t101-RPR1t-3'-fwd (DNA)125-RPR1t-tHg-Fwd (DNA)JS211-PciI-gGPM1 (DNA),+1+2100-pGPM1-5'-Rev (DNA),+1NruIPaqCIKflIODM013 (DNA)+4JS713-Cas9-AscI-R (DNA),+2NLS,45-NLS-Mxi-fwd (DNA),+2JS227-Linker-Rev (DNA)MxiI Domain,XhoI,CYC1 Terminator,+4198-Cas9_delete_fwd (DNA),+2CYC1 Terminator,BsrGI,MluIAcc65IT7,T7_promoter,M13_forward20_primer,+9BglIlacZα210-pRS426_DS_URA3_rev (DNA),+2152-f1Ori-Rev (DNA),+1161-SceI-Ura3-F (DNA)BstZ17IURA3,StuI,PspOMI,ApaIJS11-URA3-5'-Rev (DNA),BstZ17I,EcoRV,XcmIURA3 promoter,162-SceI-Ura3-R (DNA),204-KlURA3_replace_ScURA3_rev (DNA),+2

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    14231