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pDCAFB
  • Sequence Map
    Editing disabled on read-only sequence.
    tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtatactagatgaaagaacagctcaagccgctgctagaagacaagacttaccttcgccacttctggcatcccgtgtgtacccttaatgagttcgaacgcgccaacgccagtgggcacggccccaaatgccgatcgagtcaggatccatgttacgatcgatgatctcagtgtgtcctttcatatgatctactttcttgtcgagttcggcgacgatcttctgttctgaatggaagcggtgaagaccgtagggcacacatgggaattactcaagcttgcgcggttgcggtcacccgtgccggggAccIBbsINcoIAvrIIBstBICspCIBstZ17IMK2EQ4LK6PL8LE10DK12TY14LR16HF18WH20PV22CT24LN26EF28ER30AN32AS34GH36GP3865-1132ndmB CDSndmB geneJ23110iGEM15C-07iGEM15C-06BBa_...001iGEM15C-0520406080100120140160
    atgggcgtcaccttgctaggcgagaaattggtgttggccaggttaaattcaaagatcattgcggctgctgaccgatgtgctcatcgatcggcacagctctccatcggccgcgtttgcagcaacgccggaaaggactatctcgaatgcccgtatcacggctggcgctacgatgaggctgtacccgcagtggaacgatccgctctttaaccacaaccggtccaatttaagtttctagtaacgccgacgactggctacacgagtagctagccgtgtcgagaggtagccggcgcaaacgtcgttgcggcctttcctgatagagcttacgggcatagtgccgaccgcgatgctactccgacAjuIBglIClaIPvuIBspDIMG40VT42LL44GE46KL48VL50AR52LN54SK56II58AA60AD62RC64AH66RS68AQ70LS72IG74RV76CS78NA80GK82DY84LE86CP88YH90GW92RY94DE96A65-1132ndmB CDSndmB gene180200220240260280300320340
    gggcctgtcaactgatccctgcttgccctgacaaaagcatctctcctcgggccaagatttcctcattcgattgtgaggtgaaatacgacatcgtgtgggtacggctggataacagcttcgattgcactcagattccatacctcagcgatttcgataatcccgacatgcaggtaatcgtcccggacagttgactagggacgaacgggactgttttcgtagagaggagcccggttctaaaggagtaagctaacactccactttatgctgtagcacacccatgccgacctattgtcgaagctaacgtgagtctaaggtatggagtcgctaaagctattagggctgtacgtccattagcaBbvCIBfuAIBspMIEcoO109IG98AC100QL102IP104AC106PD108KS110IS112PR114AK116IS118SF120DC122EV124KY126DI128VW130VR132LD134NS136FD138CT140QI142PY144LS146DF148DN150PD152MQ154VI156V65-1132ndmB CDSndmB gene360380400420440460480500520
    tgccgattcgtatatttgggagactgttgccgagcggcggtgggagaactttacagatttttcgcactttgccttcgtacacccagggacgctctatgatccgtttttcgctagccacccaactgtttacgtgaatcgcgttgatggtgagttgcaattcaaactcgctccgccgcgtacggctaagcatataaaccctctgacaacggctcgccgccaccctcttgaaatgtctaaaaagcgtgaaacggaagcatgtgggtccctgcgagatactaggcaaaaagcgatcggtgggttgacaaatgcacttagcgcaactaccactcaacgttaagtttgagcgaggcggcgcaPasIBsaAIBsrBIA158DS160YI162WE164TV166AE168RR170WE172NF174TD176FS178HF180AF182VH184PG186TL188YD190PF192FA194SH196PT198VY200VN202RV204DG206EL208QF210KL212AP214PR21665-1132ndmB CDSndmB gene540560580600620640660680700
    gaaatgaaaggcatcccgccagaagcaccgatgggtgacttcacctaccgctgcacaatgccgtattcagtaaatcttgaaatcaaattgtggaaagatgactcacgtttcgttctttggactaccgctagccctgtcgataacaagtcttgccggaattttatgattattgtgcgtgctttactttccgtagggcggtcttcgtggctacccactgaagtggatggcgacgtgttacggcataagtcatttagaactttagtttaacacctttctactgagtgcaaagcaagaaacctgatggcgatcgggacagctattgttcagaacggccttaaaatactaataacacgcacEM218KG220IP222PE224AP226MG228DF230TY232RC234TM236PY238SV240NL242EI244KL246WK248DD250SR252FV254LW256TT258AS260PV262DN264KS266CR268NF270MI272IV274R65-1132ndmB CDSndmB gene720740760780800820840860880
    agaaggataatcaacctgatcatatgcacctggctttccagaagcgggtgcttgacgaagaccagcctgttatcgaatcgcaatggcctctcgaaatacagacctcggaagtctccgttgcaaccgataaaatttccgtccagttccgcaaatggcataaagagctatctctgtcagctcttcctattagttggactagtatacgtggaccgaaaggtcttcgcccacgaactgcttctggtcggacaatagcttagcgttaccggagagctttatgtctggagccttcagaggcaacgttggctattttaaaggcaggtcaaggcgtttaccgtatttctcgatagagacagtcgBbsIBclINdeIE276KD278NQ280PD282HM284HL286AF288QK290RV292LD294ED296QP298VI300ES302QW304PL306EI308QT310SE312VS314VA316TD318KI320SV322QF324RK326WH328KE330LS332LS334A65-1132ndmB CDSndmB gene9009209409609801,0001,0201,0401,060
    cgttgaaggacgggaggcgttccgcgattccgtcttgacgaatgttattgaagaagaacagtaatactagagtcacacaggaaagTACTAGatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggcaacttcctgccctccgcaaggcgctaaggcagaactgcttacaataacttcttcttgtcattatgatctcagtgtgtcctttcATGATCtacttgtttgaactgcagttggtcaccaaaggataacgatggtgacttctagagggcttcgcggtacagaaagtacggtgcaacaacBglIIBstXIMN2KL4DV6NQ8WF10PI12AT14TE16DL18PK20RH22VF24HA26TL28L1160-2926V336EG338RE340AF342RD344SV346LT348NV350IE352EE354Q*35665-1132ndmD CDSndmD genendmB CDSndmB geneBBa...321,0801,1001,1201,1401,1601,1801,2001,2201,240
    gggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagccccccgttctttaccggtagaccgcgctactgagaccaagtcaattacgaaccctcttggcgacgggcgtatctcctaacgccaactgtgacccacgattatggccattgctcaacgccacagtcatagtacctacctgaatactttcgccctgaccaccgacgtgaaaacagggtcgggAgeIBmrIDraIIIG30QE32MA34IW36RD38DS40GS42VN44AW46EN48RC50PH52RG54LR56LT58LG60AN62TG64NE66LR68CQ70YH72GW74TY76ES78GT80GG82CT84FV86PA881160-2926ndmD CDSndmD gene1,2601,2801,3001,3201,3401,3601,3801,4001,420
    atcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatagcgctacgtggtgggggtttacggcgcgcccaattatgaaaaggacaggcgcttttcgtgccgaaatagacctgctgtaatccagtcggcggtcctctcgggtaaagttaggagctactgcgagtcgaacatttgcgacatttttgtttagacgtatcgcatcaatatctacgactAhdIBlpINruIHR90DA92PP94PN96AA98RV100NT102FP104VR106EK108HG110FI112WT114TL116GQ118PP120GE122PI124SI126LD128DA130QL132VN134AV136KT138NL140HS142VV144ID146AD1481160-2926ndmD CDSndmD gene1,4401,4601,4801,5001,5201,5401,5601,5801,600
    tattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaaataactgcctcaacagtcgcaggatgcagtcttagaaagtcgcaagtagctacacaaaccacgccggtcgcgacttctagacgtaaactttaggtacgacgttctagctccctaagaccattgttctagtccgagataacgataagtaaaaatatacgtcgcgagttggtaatcatttAfeIAfeIClaIDrdIBglIIBspDIID150GV152VS154VL156RQ158NL160SA162FI164DV166FG168AA170SA172ED174LH176LK178SM180LQ182DR184GI186LV188TR190SG192SI194AI196HF198YM200QR202ST204IS206K1160-2926ndmD CDSndmD geneiGEM15-24C1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacaacgcaacatgtacgcgttcatgaatgaggccctgcaggtcctatgcttgaagttttcttgatgagcatacggtacttgcaataggcgtcccgtcgtcttcgacatcgatggctgaactaatcgtaatgtctatagtcgctagtctgatagcttttcgaacttcagcaatctcttttgtAclIBsaXIBsrGIEcoRVC208VV210HA212QV214LT216PG218RP220GY222EL224QK226NY228SY230AM232NV234IR236RA238AE240AV242AT244DL246IS248IT250DI252SD254QT256IE258KL260EV262VR264EN2661160-2926ndmD CDSndmD gene1,8001,8201,8401,8601,8801,9001,9201,940
    tgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaaactgattccgaggaggttgggtgatatagacgcttcaccaatgcgcatgagttctctgtccactataattgagtatgaccgacttcggatagccgatgggtgatggtcgtaagtcaggtccctacgtgtagtcgtagtgttgcggcctcccatcgtaagctgttataagggagcacttMluIBsaBIAflIIIMT268KA270PP272TH274YI276CE278VV280TR282TQ284ET286GD288IN290SY292WL294KP296IG298YP300LP302AF304SP306GM308HI310SI312TT314PE316GS318IR320QY322SL324VN3261160-2926ndmD CDSndmD gene1,9601,9802,0002,0202,0402,0602,0802,1002,120
    cgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcgcccggactgctcgcacttaggaagtagtagccacacttctttctctaagtcagggcaccgccgaggtctagttacgtgcttctacacttccaaccttgcgttgattttcaatgtgaaggctccttgccaaaaggtgagcaggtttggtcttttgtgggctaagagcatcgtccaccgBfuAIBspMIPaqCIGP328DE330RE332SF334II336GV338KK340EI342QS344RG346GS348RS350MH352ED354VK356VG358TQ360LK362VT364LP366RN368GF370PL372VQ374TR376KH378PI380LV382AG384G1160-2926ndmD CDSndmD gene2,1402,1602,1802,2002,2202,2402,2602,2802,300
    atcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaactagccatagtggggttaaaacacataccgtgtccgagacctagtcgttccaagtagcatactttatgtaataaaacgagcacgtaaactcgtacaaggtaaggtcctagctgactgacgcaacccgctagcaaacttacacgtagaaccggagccgggtctgctctgatctcgttttgPvuII386GI388TP390IL392CM394AQ396AL398DQ400QG402SS404YE406IH408YF410AR412AF414EH416VP418FQ420DR422LT424AL426GD428RL430NV432HL434GL436GP438DE440TR442AK4441160-2926ndmD CDSndmD gene2,3202,3402,3602,3802,4002,4202,4402,4602,480
    ttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaaacggctgtagtacctctaagtattgcgggttctgcatctacaaatgtgaacgccgggcgttggttactagctttgacatagacgacagcgagaacgagtaccgtagcgacttctcaggtaggctaaacttaaaaagtcatttttcttgctacaagggcaaagactacttcttatactEarIBstAPILA446DI448ME450IH452NA454QD456VD458VY460TC462GP464QP466MI468ET470VS472AV474AL476AH478GI480AE482ES484IR486FE488FF490SK492KN494DV496PV498SD500EE502YE5041160-2926ndmD CDSndmD geneiGEM15-25C2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagccaactcgagtttttttgaccagtttataagtgacagagcggaccgagatgcaacaacgttcgaacaaacctgttgctacaagcatagcttcgaagaacactcgtcccacatacgccctgaacatattgaggtcagcataggccgctagagctcgtagtactgtgaatggaaagattcBarISacIEco53kIVE506LK508KT510GQ512IF514TV516SP518GS520TL522LQ524AC526LD528ND530VR532IE534AS536CE538QG540VC542GT544CI546TP548VV550SG552DL554EH556HD558TY560LS562K1160-2926ndmD CDSndmD gene2,6802,7002,7202,7402,7602,7802,8002,8202,840
    aaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgtttctttccctttcgccattcacctagtacggcacacaaagcgcgacgttcaggtttttttagcaagagctagacactccggcccgtagtgggtgccatacctgctcgacatatttgatctctttctcctctttatgatctactagtgcgaaatactgatacttgagagccccttaacBclIPflMIMI2TL4YD6YE8LS10GN12C2989-3663K564ER566ES568GK570WI572MP574CV576SR578CK580SK582KI584VL586DL588*1160-2926EQ_gst9_1 (gBLOCK)ndmD CDSndmD geneEQ_NdmD_pSB1C3_revgst9 CDSgst9 geneEQ_pSB1C3_Gib_forBBa...BS2,8602,8802,9002,9202,9402,9602,9803,0003,020
    ctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggttgatgttccacgcctacgaggaaaggtaggacttcgatggcatgtgctatctctgtcaccttaaaaagggcaggtcccttgtatttagtggccggaaggacgcgtagttaggtgagccggtcaacggccataacgcgctactactaaagcacaacgcgctacgggtccggtaggaccaaBarIY14KV16RM18LL20SI22LK24LP26YT28IE30TV32EF34FP36SR38EH40KS42PA44FL46RI48NP50LG52QL54PV56LR58DD60DF62VL64RD66AQ68AI70LV722989-3663EQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene3,0403,0603,0803,1003,1203,1403,1603,1803,200
    tacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgatggaccgcttgtttatgctgtggtcaaactggaccataggctgtgtacgcgggcggtaggagccggtccgtttatacaccgagcgcaaacggctaccaaactggccgagctaaaggcgacgtgcaaacgtactaaacaaaaaaatgctaaagttacgtctcgttacggcacgggcgcBcgIEcoNISexAIBssHIIYL74AN76KY78DT80SL82TW84YP86TH88AP90AI92LG94QA96NM98WL100AF102AD104GL106TG108SI110SA112AR114LH116DL118FF120YD122FN124AE126QC128RA130R2989-3663gst9 CDSgst9 geneEQ_gst9_1 (gBLOCK)3,2203,2403,2603,2803,3003,3203,3403,3603,380
    cgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctgcgtgcttgatgacgcgtatgacctgcttgtggacacccgccgccttgttttacctgtagttaccgatacaggccggcggcgtgggtggtagcgcctagaacggacgaagggcatatagcgtaacagcctacttcggccgcaaagtgatgagctgatgggccgccatgctgcgaccgaNaeINgoMIVA132HE134LL136RI138LD140EH142LW144AA146EQ148NG150HQ152WL154CP156AA158AP160TI162AD164LA166CF168PY170IA172LS174DE176AG178VS180LL182DY184PA186VR188RW190L2989-3663gst9 CDSgst9 gene3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgatccggcctcacttgcggaagaaacctgtcaataatactagtagcggccgctgcagatatgctccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaacctggcacagtttgcgtaggggcctaagtggcagtacagcccctaaaaaggttggagtcgaaggaagctaggccggagtgaacgccttctttggacagttattatgatcatcgccggcgacgtctatacgaggccgttttttcccgttccacagtggtgggacgggaaaaagaaatttAjuINotIPstISpeIAvaIIBbvCIPflFITth111ID192RV194KR196IP198GF200TV202MS204GI206FP208TS210AS212FD214PA216SL218AE220ET222CQ224*2989-3663gst9 CDSgst9 geneE. coli His Operon TerminatorSuffix3,5803,6003,6203,6403,6603,6803,7003,720
    accgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaactggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttgPciIBsrBIAflIIIE. coli ...minatorVR Primer3,7403,7603,7803,8003,8203,8403,8603,8803,900
    cgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatgcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctaDrdIrep (pMB1)3,9203,9403,9603,9804,0004,0204,0404,0604,080
    acctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtApaLIrep (pMB1)4,1004,1204,1404,1604,1804,2004,2204,2404,260
    cgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgagctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactrep (pMB1)4,2804,3004,3204,3404,3604,3804,4004,4204,440
    tccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcaaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtrep (pMB1)4,4604,4804,5004,5204,5404,5604,5804,6004,620
    cctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcgagctccgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcPspXIT0 Terminator4,6404,6604,6804,7004,7204,7404,7604,7804,800
    tccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccNcoISacIEcoRVEco53kIChloramphenicol Resistance4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccPasIBsmBIEsp3IPflMIChloramphenicol Resistance5,0005,0205,0405,0605,0805,1005,1205,1405,160
    tattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttattttataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaAclIBspEIChloramphenicol Resistance5,1805,2005,2205,2405,2605,2805,3005,3205,340
    tctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctPvuIIChloramphenicol Resistance5,3605,3805,4005,4205,4405,4605,4805,500
    taactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagcattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacgggctagttgagctcacggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgBsaAIPspXIVF2 Primer5,5205,5405,5605,5805,6005,6205,6405,6605,680
    tttcgctaaggatgatttctgGAATTCGCGGCCGCTTCTAGAGaaagcgattcctactaaagacCTTAAGCGCCGGCGAAGATCTCNotIXbaIEcoRIStem LoopiGEM15C-05Prefix5,7005,720
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5739 bp42504500475050005250550025050075010001250ndmD CDSndmD genendmB CDSndmB geneiGEM15C-07iGEM15C-06point mutationiGEM15C-051160-292665-1132J23110,BBa_K082001,AvrII,AccI,BstZ17I,BbsI,CspCI,BstBI,NcoI,BglI,AjuI,BspDI,ClaI,+1EcoO109IBbvCI,BfuAI,BspMI,BsrBIPasIBsaAIBclI,NdeIBbsIBBa_B0032BglIIBstXIDraIII,BmrI,AgeINruIPspXIT0 TerminatorEco53kISacI,EcoRVNcoI,PasI,PflMI,BsmBI,Esp3IChloramphenicol ResistanceAclI,BspEIPvuIIVF2 Primer,End of Terminator,Stem Loop,Prefix,BsaAI,PspXI,EcoRI,NotI,XbaI

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    5739