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pDCAFB
  • Sequence Map
    Editing disabled on read-only sequence.
    tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtatactagatgaaagaacagctcaagccgctgctagaagacaagacttaccttcgccacttctggcatcccgtgtgtacccttaatgagttcgaacgcgccaacgccagtgggcacggcaaatgccgatcgagtcaggatccatgttacgatcgatgatctcagtgtgtcctttcatatgatctactttcttgtcgagttcggcgacgatcttctgttctgaatggaagcggtgaagaccgtagggcacacatgggaattactcaagcttgcgcggttgcggtcacccgtgccgAccIBbsIAvrIIBstBICspCIBstZ17IMK2EQ4LK6PL8LE10DK12TY14LR16HF18WH20PV22CT24LN26EF28ER30AN32AS34GH36G65-1132ndmB CDSndmB geneJ23110iGEM15C-07iGEM15C-06BBa_...001iGEM15C-0520406080100120140160
    cccatgggcgtcaccttgctaggcgagaaattggtgttggccaggttaaattcaaagatcattgcggctgctgaccgatgtgctcatcgatcggcacagctctccatcggccgcgtttgcagcaacgccggaaaggactatctcgaatgcccgtatcacggctggcgctacgatggggtacccgcagtggaacgatccgctctttaaccacaaccggtccaatttaagtttctagtaacgccgacgactggctacacgagtagctagccgtgtcgagaggtagccggcgcaaacgtcgttgcggcctttcctgatagagcttacgggcatagtgccgaccgcgatgctacAjuIBglIClaINcoIPvuIBspDIP38MG40VT42LL44GE46KL48VL50AR52LN54SK56II58AA60AD62RC64AH66RS68AQ70LS72IG74RV76CS78NA80GK82DY84LE86CP88YH90GW92RY94D65-1132ndmB CDSndmB gene180200220240260280300320340
    aggctggggcctgtcaactgatccctgcttgccctgacaaaagcatctctcctcgggccaagatttcctcattcgattgtgaggtgaaatacgacatcgtgtgggtacggctggataacagcttcgattgcactcagattccatacctcagcgatttcgataatcccgacatgcatccgaccccggacagttgactagggacgaacgggactgttttcgtagagaggagcccggttctaaaggagtaagctaacactccactttatgctgtagcacacccatgccgacctattgtcgaagctaacgtgagtctaaggtatggagtcgctaaagctattagggctgtacgtBbvCIBfuAIBspMIEcoO109IE96AG98AC100QL102IP104AC106PD108KS110IS112PR114AK116IS118SF120DC122EV124KY126DI128VW130VR132LD134NS136FD138CT140QI142PY144LS146DF148DN150PD152MQ15465-1132ndmB CDSndmB gene360380400420440460480500520
    ggtaatcgttgccgattcgtatatttgggagactgttgccgagcggcggtgggagaactttacagatttttcgcactttgccttcgtacacccagggacgctctatgatccgtttttcgctagccacccaactgtttacgtgaatcgcgttgatggtgagttgcaattcaaactcccattagcaacggctaagcatataaaccctctgacaacggctcgccgccaccctcttgaaatgtctaaaaagcgtgaaacggaagcatgtgggtccctgcgagatactaggcaaaaagcgatcggtgggttgacaaatgcacttagcgcaactaccactcaacgttaagtttgagPasIBsaAIBsrBIVI156VA158DS160YI162WE164TV166AE168RR170WE172NF174TD176FS178HF180AF182VH184PG186TL188YD190PF192FA194SH196PT198VY200VN202RV204DG206EL208QF210KL21265-1132ndmB CDSndmB gene540560580600620640660680700
    gctccgccgcgtgaaatgaaaggcatcccgccagaagcaccgatgggtgacttcacctaccgctgcacaatgccgtattcagtaaatcttgaaatcaaattgtggaaagatgactcacgtttcgttctttggactaccgctagccctgtcgataacaagtcttgccggaattttacgaggcggcgcactttactttccgtagggcggtcttcgtggctacccactgaagtggatggcgacgtgttacggcataagtcatttagaactttagtttaacacctttctactgagtgcaaagcaagaaacctgatggcgatcgggacagctattgttcagaacggccttaaaatAP214PR216EM218KG220IP222PE224AP226MG228DF230TY232RC234TM236PY238SV240NL242EI244KL246WK248DD250SR252FV254LW256TT258AS260PV262DN264KS266CR268NF27065-1132ndmB CDSndmB gene720740760780800820840860
    tgattattgtgcgtgagaaggataatcaacctgatcatatgcacctggctttccagaagcgggtgcttgacgaagaccagcctgttatcgaatcgcaatggcctctcgaaatacagacctcggaagtctccgttgcaaccgataaaatttccgtccagttccgcaaatggcataaactaataacacgcactcttcctattagttggactagtatacgtggaccgaaaggtcttcgcccacgaactgcttctggtcggacaatagcttagcgttaccggagagctttatgtctggagccttcagaggcaacgttggctattttaaaggcaggtcaaggcgtttaccgtattBbsIBclINdeIMI272IV274RE276KD278NQ280PD282HM284HL286AF288QK290RV292LD294ED296QP298VI300ES302QW304PL306EI308QT310SE312VS314VA316TD318KI320SV322QF324RK326WH328K65-1132ndmB CDSndmB gene8809009209409609801,0001,0201,040
    agagctatctctgtcagccgttgaaggacgggaggcgttccgcgattccgtcttgacgaatgttattgaagaagaacagtaatactagagtcacacaggaaagTACTAGatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccattctcgatagagacagtcggcaacttcctgccctccgcaaggcgctaaggcagaactgcttacaataacttcttcttgtcattatgatctcagtgtgtcctttcATGATCtacttgtttgaactgcagttggtcaccaaaggataacgatggtgacttctagagggcttcgcggtaBglIIE330LS332LS334AV336EG338RE340AF342RD344SV346LT348NV350IE352EE354Q*35665-1132MN2KL4DV6NQ8WF10PI12AT14TE16DL18PK20RH221160-2926ndmB CDSndmB genendmD CDSndmD geneBBa...321,0601,0801,1001,1201,1401,1601,1801,2001,220
    gtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactgcagaaagtacggtgcaacaaccccgttctttaccggtagaccgcgctactgagaccaagtcaattacgaaccctcttggcgacgggcgtatctcctaacgccaactgtgacccacgattatggccattgctcaacgccacagtcatagtacctacctgaatactttcgccctgacAgeIBmrIBstXIDraIIIVF24HA26TL28LG30QE32MA34IW36RD38DS40GS42VN44AW46EN48RC50PH52RG54LR56LT58LG60AN62TG64NE66LR68CQ70YH72GW74TY76ES78GT801160-2926ndmD CDSndmD gene1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaacaccgacgtgaaaacagggtcgggtagcgctacgtggtgggggtttacggcgcgcccaattatgaaaaggacaggcgcttttcgtgccgaaatagacctgctgtaatccagtcggcggtcctctcgggtaaagttaggagctactgcgagtcgaacatttgcgacatttttgtttAhdIBlpINruIGG82CT84FV86PA88HR90DA92PP94PN96AA98RV100NT102FP104VR106EK108HG110FI112WT114TL116GQ118PP120GE122PI124SI126LD128DA130QL132VN134AV136KT138N1160-2926ndmD CDSndmD gene1,4201,4401,4601,4801,5001,5201,5401,560
    tctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatagacgtatcgcatcaatatctacgactataactgcctcaacagtcgcaggatgcagtcttagaaagtcgcaagtagctacacaaaccacgccggtcgcgacttctagacgtaaactttaggtacgacgttctagctccctaagaccattgttctagtccgagataacgataagtaAfeIClaIDrdIBglIIBspDIL140HS142VV144ID146AD148ID150GV152VS154VL156RQ158NL160SA162FI164DV166FG168AA170SA172ED174LH176LK178SM180LQ182DR184GI186LV188TR190SG192SI194AI196H1160-2926ndmD CDSndmD gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagaaaaatatacgtcgcgagttggtaatcatttacgcaacatgtacgcgttcatgaatgaggccctgcaggtcctatgcttgaagttttcttgatgagcatacggtacttgcaataggcgtcccgtcgtcttcgacatcgatggctgaactaatcgtaatgtctatagtcgctagtctAclIAfeIBsrGIEcoRVF198YM200QR202ST204IS206KC208VV210HA212QV214LT216PG218RP220GY222EL224QK226NY228SY230AM232NV234IR236RA238AE240AV242AT244DL246IS248IT250DI252SD254Q1160-2926ndmD CDSndmD geneiGEM15-24C1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgatagcttttcgaacttcagcaatctcttttgtactgattccgaggaggttgggtgatatagacgcttcaccaatgcgcatgagttctctgtccactataattgagtatgaccgacttcggatagccgatgggtgatggtcgtaagtcaggtccctacgtgtagtcgtagtgttgMluIBsaBIBsaXIAflIIIT256IE258KL260EV262VR264EN266MT268KA270PP272TH274YI276CE278VV280TR282TQ284ET286GD288IN290SY292WL294KP296IG298YP300LP302AF304SP306GM308HI310SI312TT3141160-2926ndmD CDSndmD gene1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactccggcctcccatcgtaagctgttataagggagcacttgcccggactgctcgcacttaggaagtagtagccacacttctttctctaagtcagggcaccgccgaggtctagttacgtgcttctacacttccaaccttgcgttgattttcaatgtgaaggctccttgccaaaaggtgagPE316GS318IR320QY322SL324VN326GP328DE330RE332SF334II336GV338KK340EI342QS344RG346GS348RS350MH352ED354VK356VG358TQ360LK362VT364LP366RN368GF370PL3721160-2926ndmD CDSndmD gene2,1202,1402,1602,1802,2002,2202,2402,260
    gtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgacaggtttggtcttttgtgggctaagagcatcgtccaccgtagccatagtggggttaaaacacataccgtgtccgagacctagtcgttccaagtagcatactttatgtaataaaacgagcacgtaaactcgtacaaggtaaggtcctagctgactgacgcaacccgctagcaaactPvuIBfuAIBspMIPaqCIVQ374TR376KH378PI380LV382AG384GI386GI388TP390IL392CM394AQ396AL398DQ400QG402SS404YE406IH408YF410AR412AF414EH416VP418FQ420DR422LT424AL426GD428RL4301160-2926ndmD CDSndmD gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    atgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttacacgtagaaccggagccgggtctgctctgatctcgttttgaacggctgtagtacctctaagtattgcgggttctgcatctacaaatgtgaacgccgggcgttggttactagctttgacatagacgacagcgagaacgagtaccgtagcgacttctcaggtaggctaaacttaaEarIBstAPINV432HL434GL436GP438DE440TR442AK444LA446DI448ME450IH452NA454QD456VD458VY460TC462GP464QP466MI468ET470VS472AV474AL476AH478GI480AE482ES484IR486FE488F1160-2926ndmD CDSndmD geneiGEM15-25C2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactaaagtcatttttcttgctacaagggcaaagactacttcttatactccaactcgagtttttttgaccagtttataagtgacagagcggaccgagatgcaacaacgttcgaacaaacctgttgctacaagcatagcttcgaagaacactcgtcccacatacgccctgaacatattgaBarISacIEco53kIF490SK492KN494DV496PV498SD500EE502YE504VE506LK508KT510GQ512IF514TV516SP518GS520TL522LQ524AC526LD528ND530VR532IE534AS536CE538QG540VC542GT544CI546T1160-2926ndmD CDSndmD gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggtcagcataggccgctagagctcgtagtactgtgaatggaaagattctttctttccctttcgccattcacctagtacggcacacaaagcgcgacgttcaggtttttttagcaagagctagacactccggcccgtagtgggtgccatacctgctcgacatatttgatctctttctPflMIP548VV550SG552DL554EH556HD558TY560LS562KK564ER566ES568GK570WI572MP574CV576SR578CK580SK582KI584VL586DL588*1160-2926ndmD CDSndmD geneEQ_NdmD_pSB1C3_revEQ_gst9_1 (gBLOCK)EQ_pSB1C3_Gib_for2,8202,8402,8602,8802,9002,9202,9402,960
    ggagaaatactagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgcctctttatgatctactagtgcgaaatactgatacttgagagccccttaacgatgttccacgcctacgaggaaaggtaggacttcgatggcatgtgctatctctgtcaccttaaaaagggcaggtcccttgtatttagtggccggaaggacgcgtagttaggtgagccggtcaacBarIBclIMI2TL4YD6YE8LS10GN12CY14KV16RM18LL20SI22LK24LP26YT28IE30TV32EF34FP36SR38EH40KS42PA44FL46RI48NP50LG52QL542989-3663EQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgggccataacgcgctactactaaagcacaacgcgctacgggtccggtaggaccaaatggaccgcttgtttatgctgtggtcaaactggaccataggctgtgtacgcgggcggtaggagccggtccgtttatacaccgagcgcaaacggctaccaaactggccgagctaaaggcgacEcoNISexAIPV56LR58DD60DF62VL64RD66AQ68AI70LV72YL74AN76KY78DT80SL82TW84YP86TH88AP90AI92LG94QA96NM98WL100AF102AD104GL106TG108SI110SA1122989-3663EQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatgtgcaaacgtactaaacaaaaaaatgctaaagttacgtctcgttacggcacgggcgcgcgtgcttgatgacgcgtatgacctgcttgtggacacccgccgccttgttttacctgtagttaccgatacaggccggcggcgtgggtggtagcgcctagaacggacgaagggcatataBcgIBssHIIAR114LH116DL118FF120YD122FN124AE126QC128RA130RA132HE134LL136RI138LD140EH142LW144AA146EQ148NG150HQ152WL154CP156AA158AP160TI162AD164LA166CF168PY170I2989-3663gst9 CDSgst9 gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgatccggcctcacttgcggaagaaacctgtcaataatactagtagcgggcgtaacagcctacttcggccgcaaagtgatgagctgatgggccgccatgctgcgaccgacctggcacagtttgcgtaggggcctaagtggcagtacagcccctaaaaaggttggagtcgaaggaagctaggccggagtgaacgccttctttggacagttattatgatcatcgccAjuINaeINotISpeIAvaIIBbvCIPflFINgoMIVTth111IA172LS174DE176AG178VS180LL182DY184PA186VR188RW190LD192RV194KR196IP198GF200TV202MS204GI206FP208TS210AS212FD214PA216SL218AE220ET222CQ224*2989-3663gst9 CDSgst9 geneSuffix3,5203,5403,5603,5803,6003,6203,6403,660
    ccgctgcagatatgctccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttaggcgacgtctatacgaggccgttttttcccgttccacagtggtgggacgggaaaaagaaattttggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaatPstIBsrBIE. coli His Operon TerminatorVR Primer3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaaaggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttDrdIPciIAflIIIrep (pMB1)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcactatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtApaLIrep (pMB1)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgrep (pMB1)4,2204,2404,2604,2804,3004,3204,3404,360
    actagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctttttgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaarep (pMB1)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaatgatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcgagctccgaacctaagagtggttaPspXIT0 Te...ator4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcttttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagSacIEcoRVEco53kIT0 TerminatorChloramphenicol Resistance4,7404,7604,7804,8004,8204,8404,8604,8804,900
    acaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaatatgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatNcoIPasIBsmBIEsp3IPflMIChloramphenicol Resistance4,9204,9404,9604,9805,0005,0205,0405,060
    aaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcacttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtgAclIChloramphenicol Resistance5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaagcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagtttBspEIPvuIIChloramphenicol Resistance5,2605,2805,3005,3205,3405,3605,3805,4005,420
    atgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacgggctagttgagctcaBsaAIPspXIChloramphenicol Resistance5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctgGAATTCGCGGCCGCTTCTAGAGcggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgaaagcgattcctactaaagacCTTAAGCGCCGGCGAAGATCTCNotIXbaIEcoRIStem LoopVF2 PrimeriGEM15C-05Prefix5,6205,6405,6605,6805,7005,720
    • Plasmid
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    5739 bp42504500475050005250550025050075010001250ndmD CDSndmD genendmB CDSndmB geneiGEM15C-07iGEM15C-06point mutationiGEM15C-0565-11321160-2926

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    5739