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  • 0 feature results
pRS416gT-GalL-Cas9
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCCTAGCGAACGGACATTGAATGTGCGCGGAGCATAGAAAATTACTACCTTATTAAACCCTTAAATGAGACACAAATAAATAAAAATACAAAACATAAACCTAAAATCCEN/ARS20406080100120140160
    AAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTTTCATTTATTTCTTCCATCTTCTCAATGCCTTACTTCTTTTTTTTTATTTGTTTCCAAATTTTTTAAAGTTGTTTTTCGCATGAAATGTATATATAAATAATCTGTTCTTTTCGTCTAATTTATCTATATGTAAGCTAATTGCTATTCATTTTACATTTTAGTGTCCTAAAAGCCEN/ARS180200220240260280300320340
    TGTGTGGTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAACACACCAGAAGATGTGTCTGTTCTACTTTGTTAAGCCGTAATTATGGACTCTCGTCCTTCTCGTTCTATTTTCCATCATAAACAACCGCTAGGGGGATCTCAGAAAATGTAGAAGCCTTTTGTTTTTGATAAAAAAGAAATTAAAGAAAAAAATGAAAGATAAAAATTAAATATCEN/ARS360380400420440460480500520
    TATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTATAAATATAATTTTTTAAATTTAATATTAATAAAAATATCGTGCACTACTTTTCCTGGGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAAPmlISwaIAmpR promoterCEN/ARS540560580600620640660680700
    GAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAAmpRAmpRAmpR...ter720740760780800820840860
    CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCBcgIAmpR8809009209409609801,0001,0201,040
    GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCACTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAmpR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATABglINmeAIIIAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTAhdIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTC1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGori2,1202,1402,1602,1802,2002,2202,2402,260
    ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTGGCCTTTTGCTCgggggatctgccaattgaacataacatggtagttacatatactagtaataTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGACCGGAAAACGAGccccctagacggttaacttgtattgtaccatcaatgtatatgatcattatMfeISpeIoripRPR12,2802,3002,3202,3402,3602,3802,4002,4202,440
    tggttcggcacacattaaaagtataaaaactatctgaattacgaattacatatattggtcataaaaatcaatcaatcatcgtgtgttttatatgtctcttatctaagtataagaatatccatagttaatattcacttacgctaccttttaacctgtaatcattgtcaacaggataaccaagccgtgtgtaattttcatatttttgatagacttaatgcttaatgtatataaccagtatttttagttagttagtagcacacaaaatatacagagaatagattcatattcttataggtatcaattataagtgaatgcgatggaaaattggacattagtaacagttgtcctatpRPR12,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgttaacgacccacattgataaacgctagtatttctttttcctcttcttattggccggctgtctctatactcccctatagtctgtttcttttcgtttcgattgtccctatcagtgatagagatggcgcacatggtacgctgtggtgctcgcggctgggaacgaaactctgggagcacaattgctgggtgtaactatttgcgatcataaagaaaaaggagaagaataaccggccgacagagatatgaggggatatcagacaaagaaaagcaaagctaacagggatagtcactatctctaccgcgtgtaccatgcgacaccacgagcgccgacccttgctttgagaccctcgHpaIpRPR1RNAse P leader sequenceTetO2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgcgattggcagCGGCCGCgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttctcgagccatatccaacttccaatttaatctttcttttttaattttcacttatttgcgatacagaaagaaaaaaacgctaaccgtcGCCGGCGcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaagagctcggtataggttgaaggttaaattagaaagaaaaaattaaaagtgaataaacgctatgtctttcttttttNotIsgRNARPR1tpRPR1RNA...ce2,8202,8402,8602,8802,9002,9202,9402,960
    gcgatagtaactattgaattttgtttggatttggttagattagatatggtttctctttatatttacatgctaaaaatgggctacaccagagatacataattagatatatatacgccagtacaccttatcggcccaagccttgtcccaaggcagcgttttgttcttggaaacgctgcgctatcattgataacttaaaacaaacctaaaccaatctaatctataccaaagagaaatataaatgtacgatttttacccgatgtggtctctatgtattaatctatatatatgcggtcatgtggaatagccgggttcggaacagggttccgtcgcaaaacaagaacctttgcgacRPR1t2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccctacacgttcgctatgcttcaagaacttttctgagcacttcatgatgcatgtttgttccttattggttagctttgatgttgtgaagtcattgacacagtctgtgaaacatctttctaccagattagagtacaaacgcatgaaatccttcatttgcttttgttccactacttttgggatgtgcaagcgatacgaagttcttgaaaagactcgtgaagtactacgtacaaacaaggaataaccaatcgaaactacaacacttcagtaactgtgtcagacactttgtagaaagatggtctaatctcatgtttgcgtactttaggaagtaaacgaaaacaaggtgatgaaaaRPR1t3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tGGAACTCTTGTTGTTCTTTGCTAGCtagtcgtgcaatgtatgactttaagatttgtgagcaggaagaaaagggagaatcttctaacgataaacccttgaaaaactgggtagactacgctatgttgagttgctacgcaggctgcacaattacacgagaatgctcccgcctaggataCCTTGAGAACAACAAGAAACGATCGatcagcacgttacatactgaaattctaaacactcgtccttcttttccctcttagaagattgctatttgggaactttttgacccatctgatgcgatacaactcaacgatgcgtccgacgtgttaatgtgctcttacgagggcggatcctaBmtINheIAvrIIpGPM1RPR1t3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ttaaggctaagggacgtgcaatgcagacgacagatctaaatgaccgtgtcggtgaagtgttcgccaaacttttcggttaacacatgcagtgatgcacgcgcgatggtgctaagttacatatatatatatatGtatagccatagtgatgtctaagtaacctttatggtatatttctaattccgattccctgcacgttacgtctgctgtctagatttactggcacagccacttcacaagcggtttgaaaagccaattgtgtacgtcactacgtgcgcgctaccacgattcaatgtatatatatatataCatatcggtatcactacagattcattggaaataccatataaagaHpaIBmgBIpGPM13,5203,5403,5603,5803,6003,6203,6403,660
    taatgtggaaagatactagcgcgcgcacccacacacaagcttcgtcttttcttgaagaaaagaggaagctcgctaaatgggattccactttccgttccctgccagctgatggaaaaaggttagtggaacgatgaagaataaaaagagagatccactgaggtgaaatttcagctgaattacacctttctatgatcgcgcgcgtgggtgtgtgttcgaagcagaaaagaacttcttttctccttcgagcgatttaccctaaggtgaaaggcaagggacggtcgactacctttttccaatcaccttgctacttcttatttttctctctaggtgactccactttaaagtcgactAleIpGPM13,6803,7003,7203,7403,7603,7803,8003,8203,840
    cagcgagtttcatgatcgtgatgaacaatggtaacgagttgtggctgttgccagggagggtggttctcaacttttaatgtatggccaaatcgctacttgggtttgttatataacaaagaagaaataatgaactgattctcttcctccttcttgtcctttcttaattctgttgtaagtcgctcaaagtactagcactacttgttaccattgctcaacaccgacaacggtccctcccaccaagagttgaaaattacataccggtttagcgatgaacccaaacaatatattgtttcttctttattacttgactaagagaaggaggaagaacaggaaagaattaagacaacattpGPM1497_GPM1_FwdSeqPrime3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttaccttcctttgtaattttttttgtaattattcttcttaataatccaaacaaacacacatattacaatatattaaaatgtctagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaaaatggaaggaaacattaaaaaaaacattaataagaagaattattaggtttgtttgtgtgtataatgttatataattttacagatctaatctattttcatttcactaattgtcgcgtaatctcgacgaattactccagccttagcttccaaattgttgggcatttgagcgggtcttBarIXbaITetRpGPM14,0404,0604,0804,1004,1204,1404,1604,1804,200
    gctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactacgatccacatctcgtcggatgtaacataaccgtacattttttattcgcccgaaacgagctgcggaatcggtaactctacaatctatccgtggtatgagtgaaaacgggaaatcttcccctttcgaccgttctaaaaaatgcattattgcgattttcaaaatctacacgaaatgatSnaBITetR4,2204,2404,2604,2804,3004,3204,3404,360
    agtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagtcagtagcgctacctcgttttcatgtaaatccatgtgccggatgtctttttgtcatactttgagagcttttagttaatcggaaaaatacggttgttccaaaaagtgatctcttacgtaatatacgtgagtcgcgacaccccgtaaaatgaaatccaacgcataaccttctagttcNruITetR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    agcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttatcgtagttcagcgatttcttctttccctttgtggatgatgactatcatacggcggtaataatgctgttcgatagcttaataaactagtggttccacgtctcggtcggaagaataagccggaacttaactagtatacgcctaatctttttgttgaatttacactttcacccagaatAleITetR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agtctgaagaatgaatgatttgatgatttctttttccctccatttttcttactgaatatatcaatgatatagacttgtatagtttattatttcaaattaagtagctatatatagtcaagataacgtttgtttgacacgattacattattcgtcgacatcttttttcagcctgtcgtcagacttcttacttactaaactactaaagaaaaagggaggtaaaaagaatgacttatatagttactatatctgaacatatcaaataataaagtttaattcatcgatatatatcagttctattgcaaacaaactgtgctaatgtaataagcagctgtagaaaaaagtcggacagcSalItGPM1498_GPM1t_RevSeqPrime4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tggtagcaatttgaggagtattattaattgaataggttcattttgcgctcgcataaacagttttcgtcagggacagtatgttggaatgagtggtaattaatggtgacatgacatgttatagcaataaccttgatgtttacatcgtagtttaatgtacaccccgcgaattcgttcaaccatcgttaaactcctcataataattaacttatccaagtaaaacgcgagcgtatttgtcaaaagcagtccctgtcatacaaccttactcaccattaattaccactgtactgtacaatatcgttattggaactacaaatgtagcatcaaattacatgtggggcgcttaagcaagtBsrGIEcoRItGPM14,9204,9404,9604,9805,0005,0205,0405,060
    agtaggagtgcaccaattgcaaagggaaaagctgaatgggcagttcgaaCCGCGGTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGtcatcctcacgtggttaacgtttcccttttcgacttacccgtcaagcttGGCGCCAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCMfeISacIItGPM1211_tGPM1_RevPrime5,0805,1005,1205,1405,1605,1805,2005,2205,240
    AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACAClac promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    TGGAATTGTGAGCGGATAACAATTTCACACAggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggAGCTCGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGAACCTTAACACTCGCCTATTGTTAAAGTGTGTcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgttttcgaccTCGAGCACTGTCGGGAGGCTTCCTTCTGAGAGGAGGCACGCAGGAGCAGAAGTGGCCAGCGCAAGGACTTTGCGTCTAgeISacIEco53kIGAL-LT3_promoterM13_re...rimerlac operator5,4405,4605,4805,5005,5205,5405,5605,5805,600
    TGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATACACGGAGCGCGGCGTGACGAGGCTTGTTATTTCTAAGATGTTATGATCGAAAATACCAATACTTCTCCTTTTTAACCGTCATTGGACCGGGGTGTTTGGAAGTTTACTTGCTTAGTTTAATTGTTGGTATCCTACTATTACGCTAATCAAAAAATCGGAATAAAGACCCCATTAGAL-L5,6205,6405,6605,6805,7005,7205,7405,760
    TAATCAGCGAAGCGATGATTTTTGATCTATTAACGGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAGAAATTAGTCGCTTCGCTACTAAAAACTAGATAATTGCCTATATATTTACGTTTTTGACGTATTGGTGAAATTGATTATGAAAGTTGTAAAAGCCAAACATAATGAAGAATAAGTTTACATTATTTTCATAGTTGTTTTTTAACAATTATATGGAGATATGAAATTGCAGTTCCTCTTGAL-LGAL1_primer5,7805,8005,8205,8405,8605,8805,9005,9205,940
    AAAACCCCGGATTCTAGAACTAGTGGATCCCCCGGGaaaaaaATGGACAAGAAGTACTCCATTGGGCTCGATATCGGCACAAACAGCGTCGGCTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGGGCAATACCGATCGCCACAGCATAAAGATTTTGGGGCCTAAGATCTTGATCACCTAGGGGGCCCttttttTACCTGTTCTTCATGAGGTAACCCGAGCTATAGCCGTGTTTGTCGCAGCCGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACCCGTTATGGCTAGCGGTGTCGTATTTCTSmaISpeIXbaIXmaIBamHIEcoRVTspMINmeAIIICas95,9605,9806,0006,0206,0406,0606,0806,1006,120
    AGAACCTCATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAAAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACTCTTTCTTCCATAGGCTGGAGGATCTTGGAGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTTTCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGAGAAAGAAGGTATCCGACCTCCTKasINarISbfISfoIPluTICas96,1406,1606,1806,2006,2206,2406,2606,2806,300
    GTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATCTCGCGCTGGCGCATATGCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATATAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAGAGCGCGACCGCGTATACXcmICas96,3206,3406,3606,3806,4006,4206,4406,460
    ATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAAAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCGACAGGTTTAGGGSalIBspEICas96,4806,5006,5206,5406,5606,5806,6006,6206,640
    GGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCGACAATCTCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGCTGTTAGAPasIBsaBICas96,6606,6806,7006,7206,7406,7606,7806,8006,820
    GCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGAAGCGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACTTCBlpICas96,8406,8606,8806,9006,9206,9406,9606,9807,000
    GCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACACGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCas97,0207,0407,0607,0807,1007,1207,1407,160
    GAGAAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCTCTTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCBsu36ICas97,1807,2007,2207,2407,2607,2807,3007,3207,340
    CCCCCTCGCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACGGGGGAGCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGSmaIXmaIBsaBITspMICas97,3607,3807,4007,4207,4407,4607,4807,5007,520
    TCTCTGCTGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAGAGACGACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTBarISacIEco53kICas97,5407,5607,5807,6007,6207,6407,6607,6807,700
    AAAAGATTGAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATTTTCTAACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTPasICas97,7207,7407,7607,7807,8007,8207,8407,860
    TAGGGAGATGATTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGATCCGAGACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATATCCCTCTACTAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTABcgIKasINarISfoIBamHICspCIPluTICas97,8807,9007,9207,9407,9607,9808,0008,0208,040
    GGATTTGCCAACCGGAACTTCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGCCTAAACGGTTGGCCTTGAAGTACGTCAACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCCCTTATGACGTCTGGCAATTCCAGCACCAjuICas98,0608,0808,1008,1208,1408,1608,1808,2008,220
    ATGAACTCGTCAAAGTAATGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAATACTTGAGCAGTTTCATTACCCTTCCGTATTCGGGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTKflICas98,2408,2608,2808,3008,3208,3408,3608,3808,400
    CACCCAGCTTCAGAATGAGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGATCATATCGTGCCCCAGTCTTTTCTCAAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGGACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGATGCTGCACCTAGTATAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTCACAACTGTTCTAGGCTABmgBICas98,4208,4408,4608,4808,5008,5208,5408,560
    AAAAATAGAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCTTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGCas98,5808,6008,6208,6408,6608,6808,7008,7208,740
    AGCTTGTTGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACTCTCTCTAPmlIPsrIBstXICas98,7608,7808,8008,8208,8408,8608,8808,9008,920
    CAACAATTACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCATGFspAICas98,9408,9608,9809,0009,0209,0409,0609,0809,100
    TTCTTTTACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCNruIBspEIPaqCICas99,1209,1409,1609,1809,2009,2209,2409,260
    TTAAAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAAATTTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTKflICas99,2809,3009,3209,3409,3609,3809,4009,4209,440
    GTCTAAAAAACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACAGATTTTTTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTCas99,4609,4809,5009,5209,5409,5609,5809,6009,620
    CGAATGCTCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCAGCTTACGAGCGATCACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTCas99,6409,6609,6809,7009,7209,7409,7609,7809,800
    TCGAGCAAATAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGAAGCTCGTTTATTCGCTTAAGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTAscIBplIBplIEcoRICas99,8209,8409,8609,8809,9009,9209,9409,960
    CACCACCATAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACCCCAAGAAGAAGAGGAAGGTGTGATCTCTTCTCGAGTCATGTGTGGTGGTATCTGTCTTTCGCCATGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCTTCTCCTTCCACACTAGAGAAGAGCTCAGTACACas99,98010,00010,02010,04010,06010,08010,10010,12010,140
    AATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTATTAATCAATACAGTGCGAATGTAAGTGCGGGAGGGGGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAAAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTTAAAAAGAAAAAAAAGACATGTCTGCGCACATMluIBsrGICYC1 Terminator10,16010,18010,20010,22010,24010,26010,28010,30010,320
    CGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGgtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCGCGTACATTGTAATATGACTTTTGGAACGAACTCTTCCAAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCcatgggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGKpnIAcc65ICYC1 TerminatorlacZαT7_promoterM13_f...imerlacZ_a10,34010,36010,38010,40010,42010,44010,46010,48010,500
    TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGBglIlacZαf1 ori10,52010,54010,56010,58010,60010,62010,64010,660
    CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGAGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTf1 orilacZα10,68010,70010,72010,74010,76010,78010,80010,82010,840
    TAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTAloIf1 ori10,86010,88010,90010,92010,94010,96010,98011,00011,020
    ATTTTAACAAAATATTAACGTTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTTAGTTTTGCTGGCCTAAAATTGTTTTATAATTGCAAATGTTAAAGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATCCCATTATTGACTATATTAATTTAACTTCGAGATTAAACACTCAAATCATATGTACGTAAATGAATATTATGTCAAAAAATCAAAACGACCGGBstZ17I*268NQ266QG26411186-11989f1 oriURA311,04011,06011,08011,10011,12011,14011,16011,18011,200
    GCATCTTCTCAAATATGCTTCCCAGCCTGCTTTTCTGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACGTAGAAGAGTTTATACGAAGGGTCGGACGAAAAGACATTGCAAGTGGGAGATGGAATCGTAGGGAAGGGAAACGTTTATCAGGAGAAGGTTGTTATTATTACAGTCTAGGACATCTCTGGTGTAGTAGGTGCCAAGATATGACAACTGGGTTACGCAGAGGGAACAGTAGATTTCR262RL260YA258EW256GA254KR252YR250EG248EV246KA244DR242GK240AF238LG236RG234VI232II230DS228GT226SV224VD222DV220TR218YQ216QG214LA212DG210KD208DL20611186-11989URA311,22011,24011,26011,28011,30011,32011,34011,360
    CCCACACCGGGTGTCATAATCAACCAATCGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGGTGTGGCCCACAGTATTAGTTGGTTAGCATTGGAAGTAGAGAAGGTGGGTACAGAGAAACTCGTTATTTCGGCTATTGTTTTAGAAACAGCGAGAAGCGTTACAGTTGTCATGGGAATCATATAAGAGGTCATCTATCCCTCGGGAACGTACTGTTAAGACGATTGTAGTTTTGV204GP202TM200IL198WD196YG194ED192RG190GM188DR186QA184IF182GI180VF178DK176DS174KA172ID170VT168GK166TY164EG162TS160LS158GK156CS154LE152AL150ML148L11186-11989URA311,38011,40011,42011,44011,46011,48011,50011,52011,540
    GGCCTCTAGGTTCCTTTGTTACTTCTTCTGCCGCCTGCTTCAAACCGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAACCGGAGATCCAAGGAAACAATGAAGAAGACGGCGGACGAAGTTTGGCGATTGTTATGGACCCGGGTGGTGTGGCACACGTAAGCATTACAGACGGGTAAGACGATAAGACATATGTGGGCGTCTCATGACGTTAAACTGACATAATGGTTACAGTCGTTTAAAAGACAGAAGCTTApaIStuIPspOMIBstZ17IG146RP144EK142TV140EE138AA136QK134LG132SV130IG128PG126VV124GH122AN120TI118DA116WE114AI112RY110VG108AS106YQ104LK102VT100NG98ID96AF94KR92DE90F11186-11989URA311,56011,58011,60011,62011,64011,66011,68011,70011,720
    GAGTAAAAAATTGTACTTGGCGGATAATGCCTTTAGCGGCTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTCTCATTTTTTAACATGAACCGCCTATTACGGAAATCGCCGAATTGACACGGGAGGTACCTTTTTAGTCAGTTCTATAGGTGTACACAAAAATCATTTGTTTAAAACCCTGGATTACGAAGTTGATTGAGGTCATTAAGGAACCACCATGCTTGTAGGTTACTTCGTGTGTTCAAANcoIXcmIEcoRVL88LF86NY84KA82SL80AK78LP76KV74TG72EM70SF68DT66LI64DV62HT60KL58LC56IK54PG52LA50EV48LE46LL44EK42TT40RV38DL36SA34CL32N11186-11989URA311,74011,76011,78011,80011,82011,84011,86011,88011,900
    GTTTGCTTTTCGTGCATGATATTAAATAGCTTGGCAGCAACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATACAAACGAAAAGCACGTACTATAATTTATCGAACCGTCGTTGTCCTGATCCTACTCATCGTCGTGCAAGGAATATACATCGAAAGCTGTACTAAATAGAAGCAAAGGACGTCCAAAAACAAGACACGTCAACCCAATTCTTATGACCCGTTAAAGTACAAAGAAGTTGTGATGTATSbfIT30QK28EH26MI24NF22LK20AA18VP16SP14HT12AA10RE8KY6TA4KS2M11186-11989URA3URA3 promoter11,92011,94011,96011,98012,00012,02012,04012,060
    TGCGTATATATACCAATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGAACGCATATATATGGTTAGATTCAGACACGAGGAAGGAAGCAAGAAGGAAGACAAGCCTCTAATGGCTTAGTTTTTTTAAAGTTTCTTTGGCTTTAGTTTTTTTTCTTATTTTTTTTTTACTACTTAACTTAACTTTTCGACACCATACCACGTGAGAGTCATGTTAGACGAGACTURA3 promoter12,08012,10012,12012,14012,16012,18012,20012,22012,240
    TGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAACGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT12,26012,28012,30012,32012,34012,36012,38012,400
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12405 bp90001000011000100020003000Cas9RPR1tTetOf1 ori11186-11989112 (DNA)110 (DNA)gRNA_ds_primer (DNA)Gal1_SI_NI_PI_Rev (DNA)B5-MCS-NotI-Remove (DNA)101 (DNA)Gal1_Cas9_Fwd (DNA)87-linker-NLS-Cas9-fwd (DNA)42-5'-NLS-dCas9-fwd (DNA)Met3_Cas9_Fwd (DNA)59-Cas9-5'-Rev (DNA)Cyc1_PacI_NLS_Cas9_Rev (DNA)p414_Cas9_PacI_fwd (DNA)CYC1_fwd (DNA)66-CYC1t-5'-Fwd (DNA)CYC1-5'-REV (DNA)109-M13-fwd-REV (DNA)

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    12405