Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
working...
Advanced Settings
pRS416-dCas9-Mxi1-pRPR1(TetO)-gRNA + Tet-Repressor
  • Sequence Map
Editing disabled on read-only sequence.
GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCCTAGCGAACGGACATTGAATGTGCGCGGAGCATAGAAAATTACTACCTTATTAAACCCTTAAATGAGACACAAATAAATAAAAATACAAAACATAAACCTAAAATCCEN/ARS20406080100120140160
AAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTTTCATTTATTTCTTCCATCTTCTCAATGCCTTACTTCTTTTTTTTTATTTGTTTCCAAATTTTTTAAAGTTGTTTTTCGCATGAAATGTATATATAAATAATCTGTTCTTTTCGTCTAATTTATCTATATGTAAGCTAATTGCTATTCATTTTACATTTTAGTGTCCTAAAAGCCEN/ARS180200220240260280300320340
TGTGTGGTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAACACACCAGAAGATGTGTCTGTTCTACTTTGTTAAGCCGTAATTATGGACTCTCGTCCTTCTCGTTCTATTTTCCATCATAAACAACCGCTAGGGGGATCTCAGAAAATGTAGAAGCCTTTTGTTTTTGATAAAAAAGAAATTAAAGAAAAAAATGAAAGATAAAAATTAAATATCEN/ARS360380400420440460480500520
TATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTATAAATATAATTTTTTAAATTTAATATTAATAAAAATATCGTGCACTACTTTTCCTGGGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAAPmlISwaIAmpR promoterCEN/ARS540560580600620640660680700
GAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAAmpRAmpRAmpR...ter720740760780800820840860
CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCAmpR8809009209409609801,0001,0201,040
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCACTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAmpR1,0601,0801,1001,1201,1401,1601,1801,2001,220
TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATABglINmeAIIIAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTAhdIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTC1,5801,6001,6201,6401,6601,6801,7001,7201,740
ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAori1,7601,7801,8001,8201,8401,8601,8801,9001,920
CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTori1,9401,9601,9802,0002,0202,0402,0602,0802,100
ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGori2,1202,1402,1602,1802,2002,2202,2402,260
ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCgggggatctgccaattgaacataacatggtagttacatatactagtaatatggttcggcacTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGccccctagacggttaacttgtattgtaccatcaatgtatatgatcattataccaagccgtgMfeISpeIpRPR1oriPci1-UP2,2802,3002,3202,3402,3602,3802,4002,4202,440
acattaaaagtataaaaactatctgaattacgaattacatatattggtcataaaaatcaatcaatcatcgtgtgttttatatgtctcttatctaagtataagaatatccatagttaatattcacttacgctaccttttaacctgtaatcattgtcaacaggatatgttaacgacctgtaattttcatatttttgatagacttaatgcttaatgtatataaccagtatttttagttagttagtagcacacaaaatatacagagaatagattcatattcttataggtatcaattataagtgaatgcgatggaaaattggacattagtaacagttgtcctatacaattgctggHpaIpRPR12,4602,4802,5002,5202,5402,5602,5802,6002,620
cacattgataaacgctagtatttctttttcctcttcttattggccggctgtctctatactcccctatagtctgtttcttttcgtttcgattgtccctatcagtgatagagatggcgcacatggtacgctgtggtgctcgcggctgggaacgaaactctgggagctgcgattggcagtgtaactatttgcgatcataaagaaaaaggagaagaataaccggccgacagagatatgaggggatatcagacaaagaaaagcaaagctaacagggatagtcactatctctaccgcgtgtaccatgcgacaccacgagcgccgacccttgctttgagaccctcgacgctaaccgtpRPR1RNAse P leader sequenceTetO2,6402,6602,6802,7002,7202,7402,7602,7802,800
gCGGCCGCgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttctcgagccatatccaacttccaatttaatctttcttttttaattttcacttatttgcgatacagaaagaaaaaagcgatagtaaccGCCGGCGcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaagagctcggtataggttgaaggttaaattagaaagaaaaaattaaaagtgaataaacgctatgtctttcttttttcgctatcattgNotIsgRNARPR1t2,8202,8402,8602,8802,9002,9202,9402,960
tattgaattttgtttggatttggttagattagatatggtttctctttatatttacatgctaaaaatgggctacaccagagatacataattagatatatatacgccagtacaccttatcggcccaagccttgtcccaaggcagcgttttgttcttggaaacgctgccctacacgttataacttaaaacaaacctaaaccaatctaatctataccaaagagaaatataaatgtacgatttttacccgatgtggtctctatgtattaatctatatatatgcggtcatgtggaatagccgggttcggaacagggttccgtcgcaaaacaagaacctttgcgacgggatgtgcaaRPR1t2,9803,0003,0203,0403,0603,0803,1003,1203,140
cgctatgcttcaagaacttttctgagcacttcatgatgcatgtttgttccttattggttagctttgatgttgtgaagtcattgacacagtctgtgaaacatctttctaccagattagagtacaaacgcatgaaatccttcatttgcttttgttccactactttttggaactcttggcgatacgaagttcttgaaaagactcgtgaagtactacgtacaaacaaggaataaccaatcgaaactacaacacttcagtaactgtgtcagacactttgtagaaagatggtctaatctcatgtttgcgtactttaggaagtaaacgaaaacaaggtgatgaaaaaccttgagaacRPR1t3,1603,1803,2003,2203,2403,2603,2803,3003,320
ttgttctttCCTGCAGGtagtcgtgcaatgtatgactttaagatttgtgagcaggaagaaaagggagaatcttctaacgataaacccttgaaaaactgggtagactacgctatgttgagttgctacgcaggctgcacaattacacgagaatgctcccgcctaggatttaaggctaaacaagaaaGGACGTCCatcagcacgttacatactgaaattctaaacactcgtccttcttttccctcttagaagattgctatttgggaactttttgacccatctgatgcgatacaactcaacgatgcgtccgacgtgttaatgtgctcttacgagggcggatcctaaattccgatAvrIIpGPM13,3403,3603,3803,4003,4203,4403,4603,4803,500
agggacgtgcaatgcagacgacagatctaaatgaccgtgtcggtgaagtgttcgccaaacttttcggttaacacatgcagtgatgcacgcgcgatggtgctaagttacatatatatatatatatatatatatatatatatatagccatagtgatgtctaagtaacctttatggtatccctgcacgttacgtctgctgtctagatttactggcacagccacttcacaagcggtttgaaaagccaattgtgtacgtcactacgtgcgcgctaccacgattcaatgtatatatatatatatatatatatatatatatatatcggtatcactacagattcattggaaataccatHpaIBmgBIpGPM13,5203,5403,5603,5803,6003,6203,6403,660
tatttcttaatgtggaaagatactagcgcgcgcacccacacacaagcttcgtcttttcttgaagaaaagaggaagctcgctaaatgggattccactttccgttccctgccagctgatggaaaaaggttagtggaacgatgaagaataaaaagagagatccactgaggtgaaatttataaagaattacacctttctatgatcgcgcgcgtgggtgtgtgttcgaagcagaaaagaacttcttttctccttcgagcgatttaccctaaggtgaaaggcaagggacggtcgactacctttttccaatcaccttgctacttcttatttttctctctaggtgactccactttaaaAleIpGPM13,6803,7003,7203,7403,7603,7803,8003,8203,840
cagctgacagcgagtttcatgatcgtgatgaacaatggtaacgagttgtggctgttgccagggagggtggttctcaacttttaatgtatggccaaatcgctacttgggtttgttatataacaaagaagaaataatgaactgattctcttcctccttcttgtcctttcttaattctgtcgactgtcgctcaaagtactagcactacttgttaccattgctcaacaccgacaacggtccctcccaccaagagttgaaaattacataccggtttagcgatgaacccaaacaatatattgtttcttctttattacttgactaagagaaggaggaagaacaggaaagaattaagapGPM1497_GPM1_FwdSeqPrime3,8603,8803,9003,9203,9403,9603,9804,0004,020
gttgtaattaccttcctttgtaattttttttgtaattattcttcttaataatccaaacaaacacacatattacaatatattaaaatgtctagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcaacattaatggaaggaaacattaaaaaaaacattaataagaagaattattaggtttgtttgtgtgtataatgttatataattttacagatctaatctattttcatttcactaattgtcgcgtaatctcgacgaattactccagccttagcttccaaattgttgggcatttgagcBarITetRpGPM14,0404,0604,0804,1004,1204,1404,1604,1804,200
cccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgcgggtcttcgatccacatctcgtcggatgtaacataaccgtacattttttattcgcccgaaacgagctgcggaatcggtaactctacaatctatccgtggtatgagtgaaaacgggaaatcttcccctttcgaccgttctaaaaaatgcattattgcgattttcaaaatctacacgSnaBITetR4,2204,2404,2604,2804,3004,3204,3404,360
tttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaaaaatgattcagtagcgctacctcgttttcatgtaaatccatgtgccggatgtctttttgtcatactttgagagcttttagttaatcggaaaaatacggttgttccaaaaagtgatctcttacgtaatatacgtgagtcgcgacaccccgtaaaatgaaatccaacgcataaccttNruIBstAPITetR4,3804,4004,4204,4404,4604,4804,5004,5204,540
gatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgctagttctcgtagttcagcgatttcttctttccctttgtggatgatgactatcatacggcggtaataatgctgttcgatagcttaataaactagtggttccacgtctcggtcggaagaataagccggaacttaactagtatacgcctaatctttttgttgaatttacactttcacAleITetR4,5604,5804,6004,6204,6404,6604,6804,7004,720
ggtcttaagtctgaagaatgaatgatttgatgatttctttttccctccatttttcttactgaatatatcaatgatatagacttgtatagtttattatttcaaattaagtagctatatatagtcaagataacgtttgtttgacacgattacattattcgtcgacatcttttttcagccagaattcagacttcttacttactaaactactaaagaaaaagggaggtaaaaagaatgacttatatagttactatatctgaacatatcaaataataaagtttaattcatcgatatatatcagttctattgcaaacaaactgtgctaatgtaataagcagctgtagaaaaaagtcSalItGPM1498_GPM1t_RevSeqPrime4,7404,7604,7804,8004,8204,8404,8604,8804,900
cctgtcgtggtagcaatttgaggagtattattaattgaataggttcattttgcgctcgcataaacagttttcgtcagggacagtatgttggaatgagtggtaattaatggtgacatgacatgttatagcaataaccttgatgtttacatcgtagtttaatgtacaccccgcgaatggacagcaccatcgttaaactcctcataataattaacttatccaagtaaaacgcgagcgtatttgtcaaaagcagtccctgtcatacaaccttactcaccattaattaccactgtactgtacaatatcgttattggaactacaaatgtagcatcaaattacatgtggggcgcttaBsrGIEcoRItGPM14,9204,9404,9604,9805,0005,0205,0405,060
tcgttcaagtaggagtgcaccaattgcaaagggaaaagctgaatgggcagttcgaaCCGCGGTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAagcaagttcatcctcacgtggttaacgtttcccttttcgacttacccgtcaagcttGGCGCCAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTMfeISacIIBstAPItGPM1211_tGPM1_RevPrime5,0805,1005,1205,1405,1605,1805,2005,2205,240
GCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTACCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCCTACGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATGGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGGATlac promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
TGTTGTGTGGAATTGTGAGCGGATAACAATTtcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggAGCTCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCACAACACACCTTAACACTCGCCTATTGTTAAagtgtgtcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgttttcgaccTCGAGTATCGAAGTTTTACAAAGATGAGGAAAAAATGAGAAGGTCTAAAAGAGCCTGAGGCGCGTAGCGGTEF PromoterM13_pUC_rev_primerM13-revT3_promoterT3M13_re...rimerlac operator5,4405,4605,4805,5005,5205,5405,5605,5805,600
GTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATCATGGTGAAGTTTTGTGGGTTCGTGTCGTATGATTTAAAGGGGAGAAAGAAGGAGATCCCACAGCAATTAATGGGCATGATTTCCAAACCTTTTCTTTTTTCTCTGGCGGAGCAAAGAAAAAGAAGCAGCTTTTTCCGTTATTTTTAAAAATAGTGCAAAGAAAAAGAACTTTTATEF Promoter5,6205,6405,6605,6805,7005,7205,7405,760
TTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTCTAGAGatgtctaAAAAAAAAACTAAAAAAAGAGAAAGCTACTGGAGGGTAACTATAAATTCAATTATTTGCCAGAAGTTAAAGAGTTCAAAGTCAAAGTAAAAAGAACAAGATAATGTTGAAAAAAATGAAGAACGAGTAATCTTTCTTTCGTATCGTTAGATTAGATTCAAAAGATCTCtacagatM1S2TEF Promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
gagccccaaagaagaagagaaaagttagacccgggATGGACAAGAAGTACTCCATTGGGCTCGCTATCGGCACAAACAGCGTCGGCTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGGGCAATACCGATCGCCACAGCATAAAGAAGAACCTctcggggtttcttcttctcttttcaatctgggcccTACCTGTTCTTCATGAGGTAACCCGAGCGATAGCCGTGTTTGTCGCAGCCGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACCCGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGASmaIXmaITspMINmeAIIIMD2KK4YS6IG8LA10IG12TN14SV16GW18AV20IT22DE24YK26VP28SK30KF32KV34LG36NT38DR40HS42IK44KN46L5986-10101R3A4P5K6K7K8R9K10V11R12P13G145944-5985dCas9(Church)dCas9_VP64mNLS5,9605,9806,0006,0206,0406,0606,0806,1006,120
CATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAAAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTTTCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGAGAAAGAAGGTATCCGACCTCCTCAGGAAAKasINarISfoIPluTII48GA50LL52FD54SG56ET58AE60AT62RL64KR66TA68RR70RY72TR74RK76NR78IC80YL82QE84IF86SN88EM90AK92VD94DS96FF98HR100LE102ES104F5986-10101dCas9(Church)6,1406,1606,1806,2006,2206,2406,2606,2806,300
TTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATATAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAGAGCGCGACCGCGTATACTAGTTTAXcmIL106VE108ED110KK112HE114RH116PI118FG120NI122VD124EV126AY128HE130KY132PT134IY136HL138RK140KL142VD144ST146DK148AD150LR152LI154YL156AL158AH160MI162K5986-10101dCas9(Church)6,3206,3406,3606,3806,4006,4206,4406,460
TTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAAAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCGACAGGTTTAGGGCCGCCGASalIBspEIF164RG166HF168LI170EG172DL174NP176DN178SD180VD182KL184FI186QL188VQ190TY192NQ194LF196EE198NP200IN202AS204GV206DA208KA210IL212SA214RL216SK218SR220RL2225986-10101dCas9(Church)6,4806,5006,5206,5406,5606,5806,6006,6206,640
CGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGCTGTTAGACGACCGGPasIBsaBIEN224LI226AQ228LP230GE232KK234NG236LF238GN240LI242AL244SL246GL248TP250NF252KS254NF256DL258AE260DA262KL264QL266SK268DT270YD272DD274LD276NL278LA2805986-10101dCas9(Church)6,6606,6806,7006,7206,7406,7606,7806,8006,820
CAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACTTCCGGGAACBlpIQI282GD284QY286AD288LF290LA292AK294NL296SD298AI300LL302SD304IL306RV308NT310EI312TK314AP316LS318AS320MI322KR324YD326EH328HQ330DL332TL334LK336AL3385986-10101dCas9(Church)6,8406,8606,8806,9006,9206,9406,9606,9807,000
TCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGAAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTCTTCTVR340QQ342LP344EK346YK348EI350FF352DQ354SK356NG358YA360GY362ID364GG366AS368QE370EF372YK374FI376KP378IL380EK382MD384GT386EE388LL390VK392LN394RE396D5986-10101dCas9(Church)7,0207,0407,0607,0807,1007,1207,1407,160
TCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGBsu36IL398LR400KQ402RT404FD406NG408SI410PH412QI414HL416GE418LH420AI422LR424RQ426ED428FY430PF432LK434DN436RE438KI440EK442IL444TF446RI448PY450YV452GP454L5986-10101dCas9(Church)7,1807,2007,2207,2407,2607,2807,3007,3207,340
GCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGSmaIXmaIBsaBITspMIA456RG458NS460RF462AW464MT466RK468SE470ET472IT474PW476NF478EE480VV482DK484GA486SA488QS490FI492ER494MT496NF498DK500NL502PN504EK506VL508PK510HS512L5986-10101dCas9(Church)7,3607,3807,4007,4207,4407,4607,4807,5007,520
TGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTABarIL514YE516YF518TV520YN522EL524TK526VK528YV530TE532GM534RK536PA538FL540SG542EQ544KK546AI548VD550LL552FK554TN556RK558VT560VK562QL564KE566DY568FK570KI5725986-10101dCas9(Church)7,5407,5607,5807,6007,6207,6407,6607,6807,700
TGAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCPasIEC574FD576SV578EI580SG582VE584DR586FN588AS590LG592TY594HD596LL598KI600IK602DK604DF606LD608NE610EN612ED614IL616ED618IV620LT622LT624LF626ED628RE6305986-10101dCas9(Church)7,7207,7407,7607,7807,8007,8207,8407,860
ATGATTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGATCCGAGACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGTACTAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACKasINarISfoIBamHICspCIPluTIMI632EE634RL636KT638YA640HL642FD644DK646VM648KQ650LK652RR654RY656TG658WG660RL662SR664KL666IN668GI670RD672KQ674SG676KT678IL680DF682LK684SD686GF6885986-10101dCas9(Church)7,8807,9007,9207,9407,9607,9808,0008,0208,040
CCAACCGGAACTTCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTGGTTGGCCTTGAAGTACGTCAACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCCCTTATGACGTCTGGCAATTCCAGCACCTACTTGAAjuIAN690RN692FM694QL696IH698DD700SL702TF704KE706DI708QK710AQ712VS714GQ716GD718SL720HE722HI724AN726LA728GS730PA732IK734KG736IL738QT740VK742VV744DE746L5986-10101dCas9(Church)8,0608,0808,1008,1208,1408,1608,1808,2008,220
CGTCAAAGTAATGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGGCAGTTTCATTACCCTTCCGTATTCGGGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTGTGGGTCKflIV748KV750MG752RH754KP756EN758IV760IE762MA764RE766NQ768TT770QK772GQ774KN776SR778ER780MK782RI784EE786GI788KE790LG792SQ794IL796KE798HP800VE802NT804Q5986-10101dCas9(Church)8,2408,2608,2808,3008,3208,3408,3608,3808,400
CTTCAGAATGAGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGATGCTATCGTGCCCCAGTCTTTTCTCAAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAAATAGAAGTCTTACTCTTCGAGATGGACATGATGGACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGATGCTGCACCTACGATAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTCACAACTGTTCTAGGCTATTTTTATBmgBIL806QN808EK810LY812LY814YL816QN818GR820DM822YV824DQ826EL828DI830NR832LS834DY836DV838DA840IV842PQ844SF846LK848DD850SI852DN854KV856LT858RS860DK862N5986-10101dCas9(Church)8,4208,4408,4608,4808,5008,5208,5408,560
GAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGTCGAACAR864GK866SD868NV870PS872EE874VV876KK878MK880NY882WR884QL886LN888AK890LI892TQ894RK896FD898NL900TK902AE904RG906GL908SE910LD912KA914GF916IK918RQ920LV9225986-10101dCas9(Church)8,5808,6008,6208,6408,6608,6808,7008,7208,740
TGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACTCTCTCTAGTTGTTAPmlIPsrIBstXIET924RQ926IT928KH930VA932QI934LD936SR938MN940TK942YD944EN946DK948LI950RE952VK954VI956TL958KS960KL962VS964DF966RK968DF970QF972YK974VR976EI978NN9805986-10101dCas9(Church)8,7608,7808,8008,8208,8408,8608,8808,9008,920
TACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCTTTTATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCATGAAGAAAAFspAIYH982HA984HD986AY988LN990AV992VG994TA996LI998KK1000YP1002KL1004ES1006EF1008VY1010GD1012YK1014VY1016DV1018RK1020MI1022AK1024SE1026QE1028IG1030KA1032TA1034KY1036FF10385986-10101dCas9(Church)8,9408,9608,9809,0009,0209,0409,0609,0809,100
ACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTAAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAATTTTTNruIBspEIPaqCIYS1040NI1042MN1044FF1046KT1048EI1050TL1052AN1054GE1056IR1058KR1060PL1062IE1064TN1066GE1068TG1070EI1072VW1074DK1076GR1078DF1080AT1082VR1084KV1086LS1088MP1090QV1092NI1094VK1096K5986-10101dCas9(Church)9,1209,1409,1609,1809,2009,2209,2409,260
GACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAACTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTKflIT1098EV1100QT1102GG1104FS1106KE1108SI1110LP1112KR1114NS1116DK1118LI1120AR1122KK1124DW1126DP1128KK1130YG1132GF1134DS1136PT1138VA1140YS1142VL1144VV1146AK1148VE1150KG1152KS1154K5986-10101dCas9(Church)9,2809,3009,3209,3409,3609,3809,4009,4209,440
AAACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTTTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGK1156LK1158SV1160KE1162LL1164GI1166TI1168ME1170RS1172SF1174EK1176NP1178ID1180FL1182EA1184KG1186YK1188EV1190KK1192DL1194II1196KL1198PK1200YS1202LF1204EL1206EN1208GR1210KR1212M5986-10101dCas9(Church)9,4609,4809,5009,5209,5409,5609,5809,6009,620
TCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAGCGATCACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTL1214AS1216AG1218EL1220QK1222GN1224EL1226AL1228PS1230KY1232VN1234FL1236YL1238AS1240HY1242EK1244LK1246GS1248PE1250DN1252EQ1254KQ1256LF1258VE1260QH1262KH1264YL1266DE1268II1270EQ12725986-10101dCas9(Church)9,6409,6609,6809,7009,7209,7409,7609,7809,800
AATAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCTTATTCGCTTAAGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGAscIEcoRIIS1274EF1276SK1278RV1280IL1282AD1284AN1286LD1288KV1290LS1292AY1294NK1296HR1298DK1300PI1302RE1304QA1306EN1308II1310HL1312FT1314LT1316NL1318GA1320PA1322AF1324KY1326FD1328TT13305986-10101dCas9(Church)9,8209,8409,8609,8809,9009,9209,9409,960
ATAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACGAGGGAGCTCCCAAGAAAAAGCGCAAGGTAGGTAGTTCCAAGCTTGGCGTATCTGTCTTTCGCCATGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGCTCCCTCGAGGGTTCTTTTTCGCGTTCCATCCATCAAGGTTCGAACCGCE1G2A3P4K5K6K7R8K9V10G11S12S13K14L15G1610102-10293ID1332RK1334RY1336TS1338TK1340EV1342LD1344AT1346LI1348HQ1350SI1352TG1354LY1356ET1358RI1360DL1362SQ1364LG1366GD1368SR1370AD13725986-10101dCas9(Church)NLS9,98010,00010,02010,04010,06010,08010,10010,12010,140
GCAGCGGCGGCAGCATGGAACGTGTGAGAATGATTAATGTGCAAAGGCTGTTAGAAGCCGCAGAGTTTTTAGAAAGAAGAGAAAGAGAATGCGAACACGGGTATGCCAGTTCTTTCCCTAGCATGCCCTCTCCCAGAGGCTAACTCGAGCTCTTCTCGAGTCATGTAATTAGTTACGTCGCCGCCGTCGTACCTTGCACACTCTTACTAATTACACGTTTCCGACAATCTTCGGCGTCTCAAAAATCTTTCTTCTCTTTCTCTTACGCTTGTGCCCATACGGTCAAGAAAGGGATCGTACGGGAGAGGGTCTCCGATTGAGCTCGAGAAGAGCTCAGTACATTAATCAATSphIPspXIG17S18G19G20S21M22E23R24V25R26M27I28N29V30Q31R32L33L34E35A36A37E38F39L40E41R42R43E44R45E46C47E48H49G50Y51A52S53S54F55P56S57M58P59S60P61R62G63*6410102-10293MxiI DomainCYC1...tor10,16010,18010,20010,22010,24010,26010,28010,30010,320
TGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAAACAGTGCGAATGTAAGTGCGGGAGGGGGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAAAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTTAAAAAGAAAAAAAAGACATGTCTGCGCACATGCGTACATTMluIBsrGICYC1 Terminator10,34010,36010,38010,40010,42010,44010,46010,48010,500
CATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGGTACccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACGTAATATGACTTTTGGAACGAACTCTTCCAAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCCATGggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgtTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGKpnIAcc65ICYC1 TerminatorlacZαT7T7_promoterM13-fwdM13_f...imerlacZ_a10,52010,54010,56010,58010,60010,62010,64010,660
ATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGBglIlacZαf1 ori10,68010,70010,72010,74010,76010,78010,80010,82010,840
CGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAf1 orilacZα10,86010,88010,90010,92010,94010,96010,98011,00011,020
TTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTAloIf1 ori11,04011,06011,08011,10011,12011,14011,16011,18011,200
AAATATTAACGTTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTTTTATAATTGCAAATGTTAAAGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATCCCATTATTGACTATATTAATTTAACTTCGAGATTAAACACTCAAATCATATGTACGTAAATGAATATTATGTCAAAAAATCAAAACGACCGGCGTAGAAGABstZ17I*268NQ266QG264CR262R11352-12155URA3f1 ori11,22011,24011,26011,28011,30011,32011,34011,360
CAAATATGCTTCCCAGCCTGCTTTTCTGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGTTTATACGAAGGGTCGGACGAAAAGACATTGCAAGTGGGAGATGGAATCGTAGGGAAGGGAAACGTTTATCAGGAGAAGGTTGTTATTATTACAGTCTAGGACATCTCTGGTGTAGTAGGTGCCAAGATATGACAACTGGGTTACGCAGAGGGAACAGTAGATTTGGGTGTGGCL260YA258EW256GA254KR252YR250EG248EV246KA244DR242GK240AF238LG236RG234VI232II230DS228GT226SV224VD222DV220TR218YQ216QG214LA212DG210KD208DL206GV204G11352-12155URA311,38011,40011,42011,44011,46011,48011,50011,52011,540
GGTGTCATAATCAACCAATCGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGCCTCTAGCCACAGTATTAGTTGGTTAGCATTGGAAGTAGAGAAGGTGGGTACAGAGAAACTCGTTATTTCGGCTATTGTTTTAGAAACAGCGAGAAGCGTTACAGTTGTCATGGGAATCATATAAGAGGTCATCTATCCCTCGGGAACGTACTGTTAAGACGATTGTAGTTTTCCGGAGATCStuIP202TM200IL198WD196YG194ED192RG190GM188DR186QA184IF182GI180VF178DK176DS174KA172ID170VT168GK166TY164EG162TS160LS158GK156CS154LE152AL150ML148LG146RP14411352-12155URA311,56011,58011,60011,62011,64011,66011,68011,70011,720
GTTCCTTTGTTACTTCTTCTGCCGCCTGCTTCAAACCGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAACAAGGAAACAATGAAGAAGACGGCGGACGAAGTTTGGCGATTGTTATGGACCCGGGTGGTGTGGCACACGTAAGCATTACAGACGGGTAAGACGATAAGACATATGTGGGCGTCTCATGACGTTAAACTGACATAATGGTTACAGTCGTTTAAAAGACAGAAGCTTCTCATTTTTApaIPspOMIBstZ17IEK142TV140EE138AA136QK134LG132SV130IG128PG126VV124GH122AN120TI118DA116WE114AI112RY110VG108AS106YQ104LK102VT100NG98ID96AF94KR92DE90FL88LF8611352-12155URA311,74011,76011,78011,80011,82011,84011,86011,88011,900
ATTGTACTTGGCGGATAATGCCTTTAGCGGCTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTGTTTGCTTTTAACATGAACCGCCTATTACGGAAATCGCCGAATTGACACGGGAGGTACCTTTTTAGTCAGTTCTATAGGTGTACACAAAAATCATTTGTTTAAAACCCTGGATTACGAAGTTGATTGAGGTCATTAAGGAACCACCATGCTTGTAGGTTACTTCGTGTGTTCAAACAAACGAAANcoIXcmIEcoRVNY84KA82SL80AK78LP76KV74TG72EM70SF68DT66LI64DV62HT60KL58LC56IK54PG52LA50EV48LE46LL44EK42TT40RV38DL36SA34CL32NT30QK2811352-12155URA311,92011,94011,96011,98012,00012,02012,04012,060
TCGTGCATGATATTAAATAGCTTGGCAGCAACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATATGCGTATATAGCACGTACTATAATTTATCGAACCGTCGTTGTCCTGATCCTACTCATCGTCGTGCAAGGAATATACATCGAAAGCTGTACTAAATAGAAGCAAAGGACGTCCAAAAACAAGACACGTCAACCCAATTCTTATGACCCGTTAAAGTACAAAGAAGTTGTGATGTATACGCATATAEH26MI24NF22LK20AA18VP16SP14HT12AA10RE8KY6TA4KS2M11352-12155URA3 promoterURA312,08012,10012,12012,14012,16012,18012,20012,22012,240
ATACCAATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATATATGGTTAGATTCAGACACGAGGAAGGAAGCAAGAAGGAAGACAAGCCTCTAATGGCTTAGTTTTTTTAAAGTTTCTTTGGCTTTAGTTTTTTTTCTTATTTTTTTTTTACTACTTAACTTAACTTTTCGACACCATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATURA3 promoter12,26012,28012,30012,32012,34012,36012,38012,40012,420
GTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGACAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT12,44012,46012,48012,50012,52012,54012,560
  • Plasmid
Editing disabled on read-only sequence.
12571 bp9000100001100012000100020003000dCas9(Church)RPR1tTetOlacZ_af1 ori11352-1215510102-102935986-10101112 (DNA)135 (DNA)125 (DNA)276 (DNA)412 (DNA)487 (DNA)80 (DNA)52-sgRNA-NotI-pRPR1-R (DNA)51-pRPR1-NotI-sgRNA-F (DNA)SNR-SacI-gRNA-fwd (DNA)867-11-gRNA-DN-1 (DNA)873-11-gRNA-DN-7 (DNA)685-9-gRNA-rstitch-UP (DNA)37 SacII-gRNA-oligo (DNA)871-11-gRNA-DN-5 (DNA)gRNA_ds_primer (DNA)869-11-gRNA-DN-3 (DNA)874-11-gRNA-DN-8 (DNA)73-gRNA-5'Rev+4bp (DNA)868-11-gRNA-DN-2 (DNA)870-11-gRNA-DN-4 (DNA)719-9-q29-gRNA-L (DNA)848-11-essGuide-DN-1 (DNA)150-gRNA-outer-3'extender (DNA)720-9-q30-gRNA-R (DNA)61-gRNA-seq-rev (DNA)53-pGPM1_prom_fwd (DNA)979-13-136-site18up-GPM1-fwd (DNA)136-site18up-GPM1-fwd (DNA)92 (DNA)411 (DNA)p414_Cas9_rev (DNA)TEF1_rev (DNA)43-TEF1-5'-NLS-oligo (DNA)44-NLS-dCas9(Lu)-fwd (DNA)42-5'-NLS-dCas9-fwd (DNA)Met3_Cas9_Fwd (DNA)Cas9_D10A_fwd (DNA)Gal1_Cas9_Fwd (DNA)59-Cas9-5'-Rev (DNA)Cas9_H840A_rev (DNA)Cas9_H840A_fwd (DNA)137-site18dn-M13F-Rev (DNA)91 (DNA)57-1-pPCDA2_HygB_f (DNA)53-1-pPCDA2_for (DNA)303 (DNA)295 (DNA)297 (DNA)546 (DNA)

Welcome to Benchling!

Sign in to view data.

New digest

Saved digests

Enzyme AatII
Enzyme lists
Cut sites visible on maps
Find enzyme
Name Cuts
Selected Color
Show enzymes that cut

Primers

Version history

 Show full history
Change User Timestamp

Sequence Alignments

Saved alignments

Sequence Info

Tags

Name
Parent version
Folder
Topology
Start index
Locked
Created
--
Length
--
Export data
Split Workspace
Bases
12571