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pSpCas9(BB)-2A-Puro (PX459) V2.0
  • Sequence Map
    Editing disabled on read-only sequence.
    gagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactctcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaaccttaattaaactgacatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgahU6_promoter20406080100120140160
    atcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttGTGGAAAGGACGAAACACCggGTCTTCgaGAAGACctgttttagagctaGAAAtagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcTTTTTTgtagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaaCACCTTTCCTGCTTTGTGGccCAGAAGctCTTCTGgacaaaatctcgatCTTTatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgAAAAAAcBbsIBbsINdeIhU6_promoterLKO1_...imer180200220240260280300320340
    ttttagagctagaaatagcaagttaaaataaggctagtccgtTTTTagcgcgtgcgccaattctgcagacaaatggctctagaggtacccgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaatagtaacgccaatagggaaaaatctcgatctttatcgttcaattttattccgatcaggcaAAAAtcgcgcacgcggttaagacgtctgtttaccgagatctccatgggcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttatcattgcggttatccctKpnIXbaIAcc65ICAG_enhancer360380400420440460480500520
    ctttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattGtgcccagtacatgaccttatgggactttcctacttggcagtacatctagaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaaCacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatNdeICAG_enhancer540560580600620640660680700
    cgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgcataatcagtagcgataatggtaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccgcgcgcggtccgccccgccccgccccgNcoISnaBICAG...er720740760780800820840860
    gaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgacgctgccttcgccccgtgccccgctccgccgccctccccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgctgcgacggaagcggggcacggggcgaggcggcgg8809009209409609801,0001,0201,040
    gcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagctgagcaagaggtaagggtttaagggatggttggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcacttcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgactcgttctccattcccaaattccctaccaaccaaccaccccataattacaaattaatggacctcgtggacggactttagtgaaBseRIPaqCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tttttcaggttGGaccggtgccaccATGGACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATCaaaaagtccaaCCtggccacggtggTACCTGATATTCCTGGTGCTGCCTCTGATGTTCCTAGTACTATAACTAATGTTTCTGCTACTGCTATTCTACCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGCTGTTCTTCATGTCGTAGCCGGACCTGTAGAgeINcoIFLAGSV40 NLSFLAGFLAGSV40 NLSNLS1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAACCGTGGTTGAGACACCCGACCCGGCACTAGTGGCTGCTCATGTTCCACGGGTCGTTCTTTAAGTTCCACGACCCGTTGTGGCTGGCCGTGTCGTAGTTCTTCTTGGACTAGCCTCGGGACGACAAGCTGTCGCCGCTTTGTCGGCTCCGGTGGGCCGACTTCTCTTGGCGGTCTTBstXI1,4201,4401,4601,4801,5001,5201,5401,560
    GAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACTTCTATGTGGTCTGCCTTCTTGGCCTAGACGATAGACGTTCTCTAGAAGTCGTTGCTCTACCGGTTCCACCTGCTGTCGAAGAAGGTGTCTGACCTTCTCAGGAAGGACCACCTTCTCCTATTCTTCGTGCTCGCCGTGGGGTAGAAGCCGTTGTAGCACCTGCTCCACCGGATBglIIPflMI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCGGTGCTCTTCATGGGGTGGTAGATGGTGGACTCTTTCTTTGACCACCTGTCGTGGCTGTTCCGGCTGGACGCCGACTAGATAGACCGGGACCGGGTGTACTAGTTCAAGGCCCCGGTGAAGGACTAGCTCCCGCTGGACTTGGGGCTGTTGTCGCTGCACCTGTTCGACAAGTAGBmgBIEcoNI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGTCGACCACGTCTGGATGTTGGTCGACAAGCTCCTTTTGGGGTAGTTGCGGTCGCCGCACCTGCGGTTCCGGTAGGACAGACGGTCTGACTCGTTCTCGTCTGCCGACCTTTTAGACTAGCGGGTCGACGGGCCGCTCTTCTTCTTACCGGACAAGCCTTTGGACTAACGGGACTBstXI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGGACCCGGACTGGGGGTTGAAGTTCTCGTTGAAGCTGGACCGGCTCCTACGGTTTGACGTCGACTCGTTCCTGTGGATGCTGCTGCTGGACCTGTTGGACGACCGGGTCTAGCCGCTGGTCATGCGGCTGGACAAAGACCGGCGGTTCTTGGACAGGCTGCGGTAGGACGACTCEcoNI2,1202,1402,1602,1802,2002,2202,2402,260
    CGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCGCTGTAGGACTCTCACTTGTGGCTCTAGTGGTTCCGGGGGGACTCGCGGAGATACTAGTTCTCTATGCTGCTCGTGGTGGTCCTGGACTGGGACGACTTTCGAGAGCACGCCGTCGTCGACGGACTCTTCATGTTTCTCTAAAAGAAGCTGGTCTCGTTCTTGCCGATGCGGCCG2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCATGTAACTGCCGCCTCGGTCGGTCCTTCTCAAGATGTTCAAGTAGTTCGGGTAGGACCTTTTCTACCTGCCGTGGCTCCTTGACGAGCACTTCGACTTGTCTCTCCTGGACGACGCCTTCGTCGCCTGGAAGCTGTTGCCGTCGTAGGGGGTGGTCTAGGTGGACCCTCTCGACG2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTTGCGGTAAGACGCCGCCGTCCTTCTAAAAATGGGTAAGGACTTCCTGTTGGCCCTTTTCTAGCTCTTCTAGGACTGGAAGGCGTAGGGGATGATGCACCCGGGAGACCGGTCCCCTTTGTCGTCTAAGCGGACCTACTGGTCTTTCTCGCTCCTTTGGTAGTGGGGGACCTTGAAApaIPspOMI2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGGCTCCTTCACCACCTGTTCCCGCGAAGGCGGGTCTCGAAGTAGCTCGCCTACTGGTTGAAGCTATTCTTGGACGGGTTGCTCTTCCACGACGGGTTCGTGTCGGACGACATGCTCATGAAGTGGCACATATTGCTCGACTGGTTTCACTTTATGCACTGGCTCCCTTACTCTTTCBarI2,8202,8402,8602,8802,9002,9202,9402,960
    CCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCGGGCGGAAGGACTCGCCGCTCGTCTTTTTCCGGTAGCACCTGGACGACAAGTTCTGGTTGGCCTTTCACTGGCACTTCGTCGACTTTCTCCTGATGAAGTTCTTTTAGCTCACGAAGCTGAGGCACCTTTAGAGGCCGCACCTTCTAGCCAAGTTGCGGAGGGACCCGTGTATGGPasI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGTGCTAGACGACTTTTAATAGTTCCTGTTCCTGAAGGACCTGTTACTCCTTTTGCTCCTGTAAGACCTTCTATAGCACGACTGGGACTGTGACAAACTCCTGTCTCTCTACTAGCTCCTTGCCGACTTTTGGATACGGGTGGACAAGCTGCTGTTTCACTACTTCGTCGACTTCGCEcoRV3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCGCCTCTATGTGGCCGACCCCGTCCGACTCGGCCTTCGACTAGTTGCCGTAGGCCCTGTTCGTCAGGCCGTTCTGTTAGGACCTAAAGGACTTCAGGCTGCCGAAGCGGTTGTCTTTGAAGTACGTCGACTAGGTGCTGCTGTCGGACTGGAAATTTCTCCTGTAGGTCTTTCGGAjuI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCAGTCCACAGGCCGGTCCCGCTATCGGACGTGCTCGTGTAACGGTTAGACCGGCCGTCGGGGCGGTAATTCTTCCCGTAGGACGTCTGTCACTTCCACCACCTGCTCGAGCACTTTCACTACCCGGCCGTGTTCGGGCTCTTGTAGCACTAGCTTTACCGGTCTCTCTTGGTCTGGTSacIEco53kI3,5203,5403,5603,5803,6003,6203,6403,660
    CCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGGTCTTCCCTGTCTTCTTGTCGGCGCTCTCTTACTTCGCCTAGCTTCTCCCGTAGTTTCTCGACCCGTCGGTCTAGGACTTTCTTGTGGGGCACCTTTTGTGGGTCGACGTCTTGCTCTTCGACATGGACATGATGGACGTCTTACCCGCCCTATACATGCACCTGGTCCTTGAPflMI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCCTGTAGTTGGCCGACAGGCTGATGCTACACCTGGTATAGCACGGAGTCTCGAAAGACTTCCTGCTGAGGTAGCTGTTGTTCCACGACTGGTCTTCGCTGTTCTTGGCCCCGTTCTCGCTGTTGCACGGGAGGCTTCTCCAGCACTTCTTCTACTTCTTGATGACCGCCGTCGAC3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGGACTTGCGGTTCGACTAATGGGTCTCTTTCAAGCTGTTAGACTGGTTCCGGCTCTCTCCGCCGGACTCGCTTGACCTATTCCGGCCGAAGTAGTTCTCTGTCGACCACCTTTGGGCCGTCTAGTGTTTCGTGCACCGTGTCTAGGACCTGAGGGCCTACTTGTGATTCATGCTGCPmlICspCI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAATCTTACTGTTCGACTAGGCCCTTCACTTTCACTAGTGGGACTTCAGGTTCGACCACAGGCTAAAGGCCTTCCTAAAGGTCAAAATGTTTCACGCGCTCTAGTTGTTGATGGTGGTGCGGGTGCTGCGGATGGACTTGCGGCAGCACCCTTGGCGGGACTAGTTTTTCATGGGATT4,2204,2404,2604,2804,3004,3204,3404,360
    GCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCGACCTTTCGCTCAAGCACATGCCGCTGATGTTCCACATGCTGCACGCCTTCTACTAGCGGTTCTCGCTCGTCCTTTAGCCGTTCCGATGGCGGTTCATGAAGAAGATGTCGTTGTAGTACTTGAAAAAGTTCTGGCTCTAATGGGACCGGTTGCCGCTCTAGGCCTTCGCCGGAAjuIBmgBI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    CTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGAGACTAGCTCTGTTTGCCGCTTTGGCCCCTCTAGCACACCCTATTCCCGGCCCTAAAACGGTGGCACGCCTTTCACGACTCGTACGGGGTTCACTTATAGCACTTTTTCTGGCTCCACGTCTGTCCGCCGAAGTCGTTTCTCAGATAGGACGGGTTCTCCTTGTCGCTATTCGACTSphI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCAGCGGTCTTTCTTCCTGACCCTGGGATTCTTCATGCCGCCGAAGCTGTCGGGGTGGCACCGGATAAGACACGACCACCACCGGTTTCACCTTTTCCCGTTCAGGTTCTTTGACTTCTCACACTTTCTCGACGACCCCTAGTGGTAGTACCTTTCTTCGTCGAAGCTCTTCTTAGGBarIKflIBsaBI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    CATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGGTAGCTGAAAGACCTTCGGTTCCCGATGTTTCTTCACTTTTTCCTGGACTAGTAGTTCGACGGATTCATGAGGGACAAGCTCGACCTTTTGCCGGCCTTCTCTTACGACCGGAGACGGCCGCTTGACGTCTTCCCTTTGCTTGACCGGGACGGGAGGTTTATACACTTGAAGGACBsmIXcmI4,9204,9404,9604,9805,0005,0205,0405,060
    TACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAATGGACCGGTCGGTGATACTCTTCGACTTCCCGAGGGGGCTCCTATTACTCGTCTTTGTCGACAAACACCTTGTCGTGTTCGTGATGGACCTGCTCTAGTAGCTCGTCTAGTCGCTCAAGAGGTTCTCTCACTAGGACCGGCTGCGATTAGACCTGTTTCACGACAGGCGGATGT5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACTGTTCGTGGCCCTATTCGGGTAGTCTCTCGTCCGGCTCTTATAGTAGGTGGACAAATGGGACTGGTTAGACCCTCGGGGACGGCGGAAGTTCATGAAACTGTGGTGGTAGCTGGCCTTCTCCATGTGGTCGTGGTTTCTCCACGACCTGCGGTGGGACTAGGTGGTCTCGTAGTGXcmI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGgaattcGGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCAATGACCGAGTACGCCGGACATGCTCTGTGCCTAGCTGGACAGAGTCGACCCTCCGCTGTTTTCCGGCCGCCGGTGCTTTTTCCGGCCGGTCCGTTTTTTCTTTTTCcttaagCCGTCACCTCTCCCGTCTCCTTCAGACGATTGTACGCCACTGCAGCTCCTCTTAGGACCGGGTTACTGGCTCATGFseIBseRIEcoRIpur...t)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    AAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCATTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTPasIBsiWIPflFIRsrIIBstEIITth111Ipuro (variant)5,6205,6405,6605,6805,7005,7205,7405,760
    AGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGStuISacIIpuro (variant)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGBsaIpuro (variant)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAgaattctaaCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTcttaagattGATCTCGAGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGSacIEcoRISexAIEco53kIbGH_PA_terminatorpuro (variant)BGH_re...rimer6,1406,1606,1806,2006,2206,2406,2606,2806,300
    TGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGAgAATAGCAGGCATGCTGGGGAgcggccgcaggaacccctagtgatggagttggccactccctctctgACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTcTTATCGTCCGTACGACCCCTcgccggcgtccttggggatcactacctcaaccggtgagggagagacNotISphIbGH_PA_terminator6,3206,3406,3606,3806,4006,4206,4406,460
    cgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatSbfISmaISmaISrfISrfIXmaIXmaITspMITspMI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaagf1_origin6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattgctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataaf1_origin6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacgaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgPsiISspI7,0207,0407,0607,0807,1007,1207,1407,160
    acccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattacBsmBIBsmBIEsp3IEsp3IpGEX_3_primer7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttacaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaatSspIAmpicillinAmpR_promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ttcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacaagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgAmpicillin7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtacAmpicillin7,7207,7407,7607,7807,8007,8207,8407,860
    agtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaatcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttPvuIAmpicillin7,8807,9007,9207,9407,9607,9808,0008,0208,040
    caacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcatgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcaccttcggcgccatagtaFspISacIIAmpicillin8,0608,0808,1008,1208,1408,1608,1808,2008,220
    tgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttAmpicillin8,2408,2608,2808,3008,3208,3408,3608,3808,400
    cttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttgpBR322_origin8,4208,4408,4608,4808,5008,5208,5408,560
    caccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggapBR322_origin8,5808,6008,6208,6408,6608,6808,7008,7208,740
    gttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactcttpBR322_origin8,7608,7808,8008,8208,8408,8608,8808,9008,920
    agcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggctcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgpBR322_origin8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpBR322_origin9,1209,1409,160
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9175 bp65007000750080008500500100015002000SV40 NLSSV40 NLSNLShU6_promoter,LKO1_5_primer,NdeI,BbsI,BbsI,XbaI,Acc65I,KpnICAG_enhancerNdeI,SnaBI,NcoIFLAG,FLAG,FLAG,BseRI,PaqCI,AgeI,NcoIBstXIBglIIPflMIEcoNIBmgBI,BstXIEcoNIf1_originPsiISspIpGEX_3_primer,BsmBI,Esp3I,BsmBI,Esp3IAmpR_promoterSspIAmpicillin,PvuI,FspISacIIpBR322_origin,AflIII,PciI

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    9175