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PBS Solution

Updated 9/22/2019 01:56 AM
Step-by-step
by SoundBio iGEM (created by Keerthana Thammana)

Introduction

Recipe to make 1L of 1x PBS solution for the bacterial growth and light exposure protocol.

Materials

  • Reagents
    • 8g NaCl (above microwave)
    • 0.2g KCl (bin 1)
    • 1.44g Na2HPO4 (bin 2)
    • 0.24g KH2PO4 (bin 1)
    • Distilled water
    • HCl
  • Equipment
    • pH meter
    • Autoclave

Procedure

Procedure

  1. Start with 800mL of distilled water in a suitable container.
  1. Add 8g of NaCl to the solution.
  1. Add 0.2g of KCl to the solution.
  1. Add 1.44g of Na2HPO4 to the solution.
  1. Add 0.24g of KH2PO4 to the solution.
  1. Adjust solution to desired pH (7-7.2) by using HCl or NaOH.
  1. Add distilled water until volume is 1L.
  1. Store at room temperature.

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