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  • 0 feature results
pGL3-Basic
  • Sequence Map
    Editing disabled on read-only sequence.
    GGTACCGAGCTCTTACGCGTGCTAGCCCGGGCTCGAGATCTGCGATCTAAGTAAGCTTGGCATTCCGGTACTGTTGGTAAAGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTACCATGGCTCGAGAATGCGCACGATCGGGCCCGAGCTCTAGACGCTAGATTCATTCGAACCGTAAGGCCATGACAACCATTTCGGTGGTACCTTCTGCGGTTTTTGTATTTCTTTCCGGGCCGCGGTAAGATAGGCGACCTTCTACCTTGGCGACCTCTCGTTGACGTATTCCGATBmtIBtgIKasIKpnIMluINarINcoINheISacISfoISmaISrfIStyITliIXhoIXmaIBglIIPluTITspMIAcc65IPaeR7IEco53kIHindIIIluciferaseMCS20406080100120140160
    TGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGGACATCACTTACGCTGAGTACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTACTTCTCTATGCGGGACCAAGGACCTTGTTAACGAAAATGTCTACGTGTATAGCTCCACCTGTAGTGAATGCGACTCATGAAGCTTTACAGGCAAGCCAACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTTAGCAGCATACGTCACTTTTGAGAGAAGTTAAPfoIBstBIluciferase180200220240260280300320340
    CTTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTCGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAGCTCCCAATCATCGAAATACGGCCACAACCCGCGCAATAAATAGCCTCAACGTCAACGCGGGCGCTTGCTGTAAATATTACTTGCACTTAACGAGTTGTCATACCCGTAAAGCGTCGGATGGCACCACAAGCAAAGGTTTTTCCCCAACGTTTTTTAAAACTTGCACGTTTTTTTCGAGGGTTAGTAGBtgIluciferase360380400420440460480500520
    CAAAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTGCCAGAGTCCTTCGATAGGGACAAGACAATTGCACTGATCATGAACTCCTCTGGATCTACTGGTCTGCGTTTTTTAATAATAGTACCTAAGATTTTGCCTAATGGTCCCTAAAGTCAGCTACATGTGCAAGCAGTGTAGAGTAGATGGAGGGCCAAAATTACTTATGCTAAAACACGGTCTCAGGAAGCTATCCCTGTTCTGTTAACGTGACTAGTACTTGAGGAGACCTAGATGACCAGACGBclIBsrGIluciferase540560580600620640660680700
    CTAAAGGTGTCGCTCTGCCTCATAGAACTGCCTGCGTGAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTGATTTCCACAGCGAGACGGAGTATCTTGACGGACGCACTCTAAGAGCGTACGGTCTCTAGGATAAAAACCGTTAGTTTAGTAAGGCCTATGACGCTAAAATTCACAACAAGGTAAGGTAGTGCCAAAACCTTACAAATGATGTGAGCCTATAAACTATACACCTAAAGCTCAGCANspISphIXcmIBspEIluciferase720740760780800820840860
    CTTAATGTATAGATTTGAAGAAGAGCTGTTTCTGAGGAGCCTTCAGGATTACAAGATTCAAAGTGCGCTGCTGGTGCCAACCCTATTCTCCTTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGTGGCGCTCCCCTCTCTAAGGAATTACATATCTAAACTTCTTCTCGACAAAGACTCCTCGGAAGTCCTAATGTTCTAAGTTTCACGCGACGACCACGGTTGGGATAAGAGGAAGAAGCGGTTTTCGTGAGACTAACTGTTTATGCTAAATAGATTAAATGTGCTTTAACGAAGACCACCGCGAGGGGAGAGATTCSapIBspQIluciferase8809009209409609801,0001,0201,040
    GAAGTCGGGGAAGCGGTTGCCAAGAGGTTCCATCTGCCAGGTATCAGGCAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGACTTCAGCCCCTTCGCCAACGGTTCTCCAAGGTAGACGGTCCATAGTCCGTTCCTATACCCGAGTGACTCTGATGTAGTCGATAAGACTAATGTGGGCTCCCCCTACTATTTGGCCCGCGCCAGCCATTTCAACAAGGTAAAAAACTTCGCTTCCAACACCTAGACCTATGGCCCTluciferase1,0601,0801,1001,1201,1401,1601,1801,2001,220
    AAACGCTGGGCGTTAATCAAAGAGGCGAACTGTGTGTGAGAGGTCCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATCGTTGACCGCCTTTTGCGACCCGCAATTAGTTTCTCCGCTTGACACACACTCTCCAGGATACTAATACAGGCCAATACATTTGTTAGGCCTTCGCTGGTTGCGGAACTAACTGTTCCTACCTACCGATGTAAGACCTCTGTATCGAATGACCCTGCTTCTGCTTGTGAAGAAGTAGCAACTGGCGGABseYIBspEIPpuMIEcoO109Iluciferase1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GAAGTCTCTGATTAAGTACAAAGGCTATCAGGTGGCTCCCGCTGAATTGGAATCCATCTTGCTCCAACACCCCAACATCTTCGACGCAGGTGTCGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAACTTCAGAGACTAATTCATGTTTCCGATAGTCCACCGAGGGCGACTTAACCTTAGGTAGAACGAGGTTGTGGGGTTGTAGAAGCTGCGTCCACAGCGTCCAGAAGGGCTGCTACTGCGGCCACTTGAAGGGCGGCGGCAACAACAAAACCTCGTGCCTTTCTGCTACTGCCTTTTTPaqCISgrAIluciferase1,4201,4401,4601,4801,5001,5201,5401,560
    GAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGATCGCCGTGTAATTCTAGAGTCCTCTAGCACCTAATGCAGCGGTCAGTTCATTGTTGGCGCTTTTTCAACGCGCCTCCTCAACACAAACACCTGCTTCATGGCTTTCCAGAATGGCCTTTTGAGCTGCGTTCTTTTTAGTCTCTCTAGGAGTATTTCCGGTTCTTCCCGCCTTTCTAGCGGCACATTAAGATCTCAGBplIBplIXbaIluciferase1,5801,6001,6201,6401,6601,6801,7001,7201,740
    GGGGCGGCCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTCCCCGCCGGCCGGCGAAGCTCGTCTGTACTATTCTATGTAACTACTCAAACCTGTTTGGTGTTGATCTTACGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAACGAAATAAACATTGGTAATATTCGACGTTATTTGTTCAATTGTTGTTGTTAACGTAAGTAAAFseIHpaISV40 poly(A) signal1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAAGGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTATACAAAGTCCAAGTCCCCCTCCACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGTTTACACCATTTTAGCTATTCCTAGGCAGCTGGCTACGGGAACTCTCGGAAGTTGGGTCAGTCGAGGAAGGCCACCCGCGCCCCGTACTGATAGCAGCGGCGTGAATACTGACAGAAAccISalIBamHIBsaBIPshAI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCAfeINspIPciISapIBspQI2,1202,1402,1602,1802,2002,2202,2402,260
    CCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAABssSIBssSαIori2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGApaLIBaeGIBseYIori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGAlwNIori2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAori2,8202,8402,8602,8802,9002,9202,9402,960
    TTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAAhdIBglIBsaIBsrDINmeAIIIAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCAclIAseIFspIBsrDIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGACAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTPvuIAmpR3,5203,5403,5603,5803,6003,6203,6403,660
    GTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAclIXmnIApaLIBaeGIBssSIBssSαIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTAAmpR promoterAmpRAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGTTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCf1 oriAmpR promoter4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAAloIBsaAIBtgZIDraIIIf1 ori4,2204,2404,2604,2804,3004,3204,3404,360
    CTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTGCCATTCGCCATTCAGGCTGCGCGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCGAATGTTAAACGGTAAGCGGTAAGTCCGACGCGBglIFspIf1 ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    AACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCCCAAGCTACCATGATAAGTAAGTAATATTAAGGTACGGGAGGTACTTGGAGCGGCCGCAATAAAATATCTTTATTTTCATTACATCTGTGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATATTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGGGTTCGATGGTACTATTCATTCATTATAATTCCATGCCCTCCATGAACCTCGCCGGCGTTATTTTATAGAAATAAAAGTAATGTAGACACACAACCAAAAAACACACTTAGCTATCATGATTGTATNotIPvuIpoly(A) signal4,5604,5804,6004,6204,6404,6604,6804,7004,720
    CGCTCTCCATCAAAACAAAACGAAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCTATCGATAGCGAGAGGTAGTTTTGTTTTGCTTTGTTTTGTTTGATCGTTTTATCCGACAGGGGTCACGTTCACGTCCACGGTCTTGTAAAGAGATAGCTATBsgIPaqCIpause site4,7404,7604,7804,800
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4818 bp350037504000425045002505007501000MCS,Acc65I,KpnI,Eco53kI,SacI,MluI,NheI,BmtI,TspMI,XmaI,SmaI,SrfI,PaeR7I,TliI,+12BtgIBsrGIBclI,NspI,SphIBspEI,XcmI,BspQI,SapIluciferaseAclI,XmnI,BssSI,BssSαIAmpR,ApaLI,BaeGIAmpR promoterf1 ori,BtgZI,BsaAI,DraIIIAloIpoly(A) signal,pause site,BglI,FspI,PvuI,NotI,PaqCI,BsgI

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    4818