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pLVX-TetOne-Puro (Archived)
  • Sequence Map
    Editing disabled on archived sequence.
    tggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaaccttcccgattaagtgagggtttcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaatcgtcttgatgtgtggtcccggtccccagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcggtctattccatcttct3' long terminal repeat (LTR) from HIV-120406080100120140160
    ggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagccggttatttcctctcttgtggtcgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcacaatctcacctccaaactgtcggcggatcgtaaagtagtgcaccgggctctcgacgtaggcctcatgaagttcttgacgactatagctcgaacgatgttc3' long terminal repeat (LTR) from HIV-1180200220240260280300320340
    ggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcctgaaaggcgacccctgaaaggtccctccgcaccggacccgccctgacccctcaccgctcgggagtctaggacgtatattcgtcgacgaaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggaAflII3' long terminal repeat (LTR) from HIV-1360380400420440460480500520
    caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgcgtcctgagc3' long terminal repeat (LTR) from HIV-1packagi...type 1540560580600620640660680700
    gcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaacgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttcttttttNruIpackaging signal of human immunodeficiency virus type 1720740760780800820840860
    tataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgggagataa8809009209409609801,0001,0201,040
    gtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcatFseIMfeISapIBspQI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtattttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatThe Rev response element (RRE) of HIV-1 allows...RNA export from the nucleus to the cytoplasm.1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccacatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgBbvCIBpu10IThe Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.1,4201,4401,4601,4801,5001,5201,5401,560
    tgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagatacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctaAleI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggtt1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagagctgccatagcggaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgKflIPpuMIcentral polypurine tract and cent...e of HIV-1 (lacking the first T)1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aaactaaagaactacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttatcgacttaacttgtttattgcagcttataatggttacaaataaggcaatagcatcacaaatttcacaaataaggcatttttttcactgcattctagtttgatttcttgatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcaaatagctgaattgaacaaataacgtcgaatattaccaatgtttattccgttatcgtagtgtttaaagtgtttattccgtaaaaaaagtgacgtaagatcSV40 polyadenylation signalcentral polypurine ...cking the first T)2,1202,1402,1602,1802,2002,2202,2402,260
    ttttggtttgtccaaactcatcaatgtatcttatcatgtctggatctcaaatccctcggaagctgcgcctgtcttaggttggagtgatacatttttatcacttttacccgtctttggattaggcagtagctctgacggccctcctgtcttaggttagtgaaaaatgtcactctctaaaaccaaacaggtttgagtagttacatagaatagtacagacctagagtttagggagccttcgacgcggacagaatccaacctcactatgtaaaaatagtgaaaatgggcagaaacctaatccgtcatcgagactgccgggaggacagaatccaatcactttttacagtgagagaSV40 polyadenylation signal2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tacccgtcattggctgtccagcttagctcgcaggggaggtggtctggatccgccggcaccggtgtatacgggaattctttacgagggtaggaagtggtacggaaagttggtataagacaaaagtgttgtggaattgaagtttactcaaaaaatcagcactcttttataggcgcccatgggcagtaaccgacaggtcgaatcgagcgtcccctccaccagacctaggcggccgtggccacatatgcccttaagaaatgctcccatccttcaccatgcctttcaaccatattctgttttcacaacaccttaacttcaaatgagttttttagtcgtgagaaaatatccgcgggAgeIBlpIBamHIEcoRISgrAIBstZ17I3rd-generation Tet-responsive promoter that ca...or endogenous mammalian transcription factorsmultiple cloning site2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tggtttacataagcaaagcttatacgttctctatcactgatagggagtaaactggatatacgttctctatcactgatagggagtaaactgtagatacgttctctatcactgatagggagtaaactggtcatacgttctctatcactgatagggagtaaactccttatacgttctcaccaaatgtattcgtttcgaatatgcaagagatagtgactatccctcatttgacctatatgcaagagatagtgactatccctcatttgacatctatgcaagagatagtgactatccctcatttgaccagtatgcaagagatagtgactatccctcatttgaggaatatgcaagag3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On(R) 3G, modified to eliminatebinding sites for endogenous mammalian transcription factorstetO p..._bindtetO p..._bindtetO p..._bindtetO p..._bind2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tatcactgatagggagtaaagtctgcatacgttctctatcactgatagggagtaaactcttcatacgttctctatcactgatagggagtaaactcgaggtgataattccacggggttggggttgcgccttttccaaggcagccctgggtttgcgcagggacgcggctgctctgggatagtgactatccctcatttcagacgtatgcaagagatagtgactatccctcatttgagaagtatgcaagagatagtgactatccctcatttgagctccactattaaggtgccccaaccccaacgcggaaaaggttccgtcgggacccaaacgcgtccctgcgccgacgagacccFspITliIXhoIBstXIPspXIPaeR7I3rd-generation Tet-responsive promoter that ... endogenous mammalian transcription factorshuman phosphoglycerate kinase 1 promotertetO p..._bindtetO p..._bindtetO...ind2,8202,8402,8602,8802,9002,9202,9402,960
    cgtggttccgggaaacgcagcggcgccgaccctgggtctcgcacattcttcacgtccgttcgcagcgtcacccggatcttcgccgctacccttgtgggccccccggcgacgcttcctgctccgcccctaagtcgggaaggttccttgcggttcgcggcgtgccggacgtgacaaagcaccaaggccctttgcgtcgccgcggctgggacccagagcgtgtaagaagtgcaggcaagcgtcgcagtgggcctagaagcggcgatgggaacacccggggggccgctgcgaaggacgaggcggggattcagcccttccaaggaacgccaagcgccgcacggcctgcactgtttAhdIhuman phosphoglycerate kinase 1 promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cggaagccgcacgtctcactagtaccctcgcagacggacagcgccagggagcaatggcagcgcgccgaccgcgatgggctgtggccaatagcggctgctcagcagggcgcgccgagagcagcggccgggaaggggcggtgcgggaggcggggtgtggggcggtagtgtgggccctgccttcggcgtgcagagtgatcatgggagcgtctgcctgtcgcggtccctcgttaccgtcgcgcggctggcgctacccgacaccggttatcgccgacgagtcgtcccgcgcggctctcgtcgccggcccttccccgccacgccctccgccccacaccccgccatcacacccgggaAscIBlpIMscISpeIBsmBIEsp3Ihuman phosphoglycerate kinase 1 promoter3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gttcctgcccgcgcggtgttccgcattctgcaagcctccggagcgcacgtcggcagtcggctccctcgttgaccgaatcaccgacctctctccccagggggatcatcgaattaccatgtctagactggacaagagcaaagtcataaactctgctctggaattactcaatggagtccaaggacgggcgcgccacaaggcgtaagacgttcggaggcctcgcgtgcagccgtcagccgagggagcaactggcttagtggctggagagaggggtccccctagtagcttaatggtacagatctgacctgttctcgtttcagtatttgagacgagaccttaatgagttacctcagXbaIhuman phosphoglycerate kinase 1 promoterTet-On(R) 3G3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggtatcgaaggcctgacgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacgtgaagaacaagcgggccctgctcgatgccctgccaatcgagatgctggacaggcatcatacccactcctgccccctggaaggcgagtcatggcaagactttcccatagcttccggactgctgttcctttgagcgagttttcgaccctcaactcgtcggatgggacatgaccgtgcacttcttgttcgcccgggacgagctacgggacggttagctctacgacctgtccgtagtatgggtgaggacgggggaccttccgctcagtaccgttctgaaagTet-On(R) 3G3,5203,5403,5603,5803,6003,6203,6403,660
    tgcggaacaacgccaagtcataccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcacccgcccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtacgccttgttgcggttcagtatggcgacacgagaggagagtgtagcgctgccccgatttcacgtagagccgtgggcgggttgtctctttgtcatgctttgggaccttttagtcgagcgcaaggacacagtcgttccgaagagggacctcttgcgtgacatgcgagacaggcggcaNruIPsrITet-On(R) 3G3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagaggaaagagagacacctaccaccgattctatgcccccacttctgaaacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatatgtcccggtgaaatgtgacccgacgcataacctccttgtcctcgtagttcatcgttttctcctttctctctgtggatggtggctaagatacgggggtgaagactttgttcgttaactcgacaagctggccgtccctcggcttggacggaaggaaaagccggaccttgattagtatacaAloIMfeINdeIBfuAIBspMITet-On(R) 3G3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggcctggagaaacagctaaagtgcgaaagcggcgggccgaccgacgcccttgacgattttgacttagacatgctcccagccgatgcccttgacgactttgaccttgatatgctgcctgctgacgctcttgacgattttgaccttgacatgctccccgggtaaacgcgcgaatgtgccggacctctttgtcgatttcacgctttcgccgcccggctggctgcgggaactgctaaaactgaatctgtacgagggtcggctacgggaactgctgaaactggaactatacgacggacgactgcgagaactgctaaaactggaactgtacgaggggcccatttgcgcgcttacacSmaIXmaITspMITet-On(R) 3G4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgNsiINsiISphISphISexAISV40 enhancer and early promoter4,2204,2404,2604,2804,3004,3204,3404,360
    ccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaacgcgaccatgaccgagtacaagggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgttttgcgctggtactggctcatgttcNcoISfiIAvrIISV40 enhancer and early promoterSV40 origin of replicationpac ...CDS4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaagggggtgccacgcggagcggtgggcgctgctgcagggggcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctgggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccSmaIXmaIBsiWIPflFIRsrIITspMIBstEIITth111Ipac from Streptomyces alboniger CDS4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcacacccagcgcctgctgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgSacIIpac from Streptomyces alboniger CDS4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgcccaccaaggaccggtggcagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggBcgIMscIBsmBIEsp3Ipac from Streptomyces alboniger CDS4,9204,9404,9604,9805,0005,0205,0405,060
    gacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgtctggaacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgttcgggccacggacttgcgcagaccttgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacataMluISexAIAflIIIwoodchuck hepatitis virus posttranscriptional regulatory elementpac from Streptomyces alboniger CDS5,0805,1005,1205,1405,1605,1805,2005,2205,240
    catgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccggtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggcPflMIwoodchuck hepatitis virus posttranscriptional regulatory element5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggaccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgNcoIwoodchuck hepatitis virus posttranscriptional regulatory element5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcggaccttaattaagacgtcagctctggatctttttgtacPstISacIIwoodchuck hepatitis virus posttranscriptional regulatory element5,6205,6405,6605,6805,7005,7205,7405,760
    gagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggtttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaagaggggactggaagctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttctcccctgaccttcKpnIAcc65IBsu36I5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggccaaccgattaagtgagggttgcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaatcgtcttgatgtgtggtcccggtccccagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcggtctattccatcttctccggtt3' long terminal repeat (LTR) from HIV-15,9605,9806,0006,0206,0406,0606,0806,1006,120
    taaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggacttatttcctctcttgtggtcgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcacaatctcacctccaaactgtcggcggatcgtaaagtagtgcaccgggctctcgacgtaggcctcatgaagttcttgacgactatagctcgaacgatgttccctgaa3' long terminal repeat (LTR) from HIV-16,1406,1606,1806,2006,2206,2406,2606,2806,300
    tccgctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaaggcgacccctgaaaggtccctccgcaccggacccgccctgacccctcaccgctcgggagtctaggacgtatattcgtcgacgaaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttattAflII3' long terminal repeat (LTR) from HIV-16,3206,3406,3606,3806,4006,4206,4406,460
    agcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggctcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtcataaatattgaacgtttctttacttatagtctctcactctccg3' long terminal repeat (LTR) from HIV-16,4806,5006,5206,5406,5606,5806,6006,6206,640
    cttgacattgctagcgttttaccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattggaactgtaacgatcgcaaaatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacBmtINheI6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaagcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttSapIBspQI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgDrdIPciIAflIIIhigh-copy-number ColE1/pMB1/pBR322/pUC origin of replication7,0207,0407,0607,0807,1007,1207,1407,160
    ccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggtBssSIBssSαIhigh-copy-number ColE1/pMB1/pBR322/pUC origin of replication7,1807,2007,2207,2407,2607,2807,3007,3207,340
    agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtgtcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccachigh-copy-number ColE1/pMB1/pBR322/pUC origin of replication7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctahigh-copy-number ColE1/pMB1/pBR322/pUC origin of replication7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgctggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaBspHIAmpR7,7207,7407,7607,7807,8007,8207,8407,860
    taatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggAhdIAmpR7,8807,9007,9207,9407,9607,9808,0008,0208,040
    agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattctcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagAclIFspIAmpR8,0608,0808,1008,1208,1408,1608,1808,2008,220
    agctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaPvuIAmpR8,2408,2608,2808,3008,3208,3408,3608,3808,400
    tttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgaAclIBcgIAmpR8,4208,4408,4608,4808,5008,5208,5408,560
    gttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataactSspIBssSIBssSαIAmpRbla promoter8,5808,6008,6208,6408,6608,6808,7008,7208,740
    agcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcaacttgtttattgcagcttataatggttacaaataaagcaatagcatctcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagBspHISgrDIbla promoterSV40 polyadenylation signal8,7608,7808,8008,8208,8408,8608,8808,9008,920
    acaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgccaattacctagtggtttcatttatgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctagttgacctattgagttcgattggttttagtagggtttgaagggtggggtatgggataatggtgacggttaatggatcaccaaagtaaatSV40 polyadenylation signal8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ctctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatgtactacaaacttagtagtttttaaagaaattgtatttgttaaatatgtactacaaacttagtagtgagatttggacactaaggagacttaataaaagtaaaatttctttaacataaacaatttatacatgatgtttgaatcatcaaaaatttctttaacataaacaatttatacatgatgtttgaatcatca9,1209,1409,1609,1809,2009,220
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on archived sequence.
    9227 bp650070007500800085005001000150020003' long terminal repeat (LTR) from HIV-1,AflIIpackaging signal of human immunodeficiency virus type 1,NruIBspQI,SapI,FseIMfeI+1BbvCI,Bpu10I,AleIKflI,PpuMI+1SV40 polyadenylation signalBspQI,SapI,AflIII,PciIDrdI,BssSI,BssSαIhigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationBspHIAhdIAmpR,FspI,AclI,PvuIBcgI,AclI,BssSI,BssSαIbla promoter,SV40 polyadenylation signal,SspI,BspHI,SgrDI

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