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pGLO sequence
  • Sequence Map
Editing disabled on read-only sequence.
atcgatgcataatgtgcctgtcaaatggacgaagcagggattctgcaaaccctatgctactccgtcaagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcatttagctacgtattacacggacagtttacctgcttcgtccctaagacgtttgggatacgatgaggcagttcggcagttaacagactaagcaatggttaatactgttgaactgccgatgtagtaagtgaaaaagaagtgttggccgtgccttgagcgagcccgaccggggccacgtaaClaINsiIBspDIBstAPIarabinose\C\gene20406080100120140160
ttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgaaaaatttatgggcgctctttatctcaactagcagttttggttgtaacgctggctgccaccgctatccgtaggcccaccacgagttttcgtcgaagcggaccgactatgcaaccaggagcgcggtcgaattctgcgattagggattgacgaccgccttttctacactgtctgcgctBaeIAflIIBsaBIarabinose\C\gene180200220240260280300320340
cggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcgccgctgttcgtttgtacgacacgctgcgaccgctatagttttaacgacagacggtccactagcgactacatgactgttcggagcgcatgggctaataggtagccacctacctcgctgagcaattagcgaaggtacgcggcgtcattgttaacgagttcgtctaaatagcggtcgEcoRVarabinose\C\gene360380400420440460480500520
agctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgattcgaggcttatcgcgggaaggggaacgggccgcaattactaaacgggtttgtccagcgactttacgccgaccacgcgaagtaggcccgctttcttggggcataaccgtttataactgccggtcaattcggtaagtacggtcatccgcgcgcctgctttcatttgggtgaccactaAjuIBssHIIarabinose\C\gene540560580600620640660680700
accattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaatggtaagcgctcggaggcctactgctggcatcactacttagagaggaccgcccttgtcgttttatagtgggccagccgtttgtttaagagcagggactaaaaagtggtgggggactggcgcttaccactctaactcttatattggaaagtaagggtcgccagccagctattttttNruIBspEIarabinose\C\gene720740760780800820840860
atcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttagctctattggcaaccggagttagccgcaatttgggcggtggtctacccgtaatttgctcatagggccgtcgtcccctagtaaaacgcgaagtcggtatgaaaagtatgagggcggtaagtctcttctttggttaacaggtataacgtagtctgtaacggcagtgacgcagaaaPflMIarabinose\C\genearabinose\promoter8809009209409609801,0001,0201,040
tactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatatgaccgagaagagcgattggtttggccattggggcgaataattttcgtaagacattgtttcgccctggtttcggtactgtttttgcgcattgttttcacagatattagtgccgtcttttcaggtgtaactaataaacgtgccgcagtgtgaaacgatacggtatcgtaaaaataAgeIBsmIMluISapIBspQIBstEIIarabinose\promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
ccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagaaataattttgtttaactttaagaaggagatatacatatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagaggtattctaatcgcctaggatggactgcgaaaaatagcgttgagagatgacaaagaggtatgggcaaaaaaacccgatctttattaaaacaaattgaaattcttcctctatatgtataccgatcgtttcctcttcttgaaaagtgacctcaacagggttaagaacaacttaatctBarIBmtIBpmINheIBsaXIGFP\in\pGLOarabinose\promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
tggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcataccactacaattacccgtgtttaaaagacagtcacctctcccacttccactacgatgtatgcctttcgaatgggaatttaaataaacgtgatgaccttttgatggacaaggtaccggttgtgaacagtgatgaaagagaataccacaagttacgaaaagggcaataggcctagtaBtgIMscINcoIStyIBspEIHindIIIGFP\in\pGLO1,4201,4401,4601,4801,5001,5201,5401,560
atgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacatactttgccgtactgaaaaagttctcacggtacgggcttccaatacatgtccttgcgtgatatagaaagtttctactgcccttgatgttctgcgcacgacttcagttcaaacttccactatgggaacaattagcatagctcaattttccataactaaaatttcttctacctttgtBaeIBarIMluIBsrGIGFP\in\pGLOF\and\R...is\GFP1,5801,6001,6201,6401,6601,6801,7001,7201,740
ttctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctaagagcctgtgtttgagctcatgttgatattgagtgtgttacatatgtagtgccgtctgtttgttttcttaccttagtttcgattgaagttttaagcggtgttgtaacttctacctaggcaagttgatcgtctggtaatagttgttttatgaggttaaccgctaccgggacaggaTliIXhoIPspXIPaeR7IBstZ17IGFP\in\pGLO1,7601,7801,8001,8201,8401,8601,8801,9001,920
tttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatgaattcgagctcggtacccggggatcctctagagtcgaaaatggtctgttggtaatggacagctgtgttagacgggaaagctttctagggttgcttttcgcactggtgtaccaggaagaactcaaacattgacgacgaccctaatgtgtaccgtacctactcgagatgtttattacttaagctcgagccatgggcccctaggagatctcagctKpnISacISacISalISalISmaIXbaIXmaIBstBIBtgZIEcoRITspMIAcc65IEco53kIEco53kIGFP\in\pGLO1,9401,9601,9802,0002,0202,0402,0602,0802,100
cctgcaggcatgcaagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggacgtccgtacgttcgaaccgacaaaaccgcctactctcttctaaaagtcggactatgtctaatttagtcttgcgtcttcgccagactattttgtcttaaacggaccgccgtcatcgcgccaccagggtggactggggtacggcttgagtcttcactttgcggcatcgcggctaPstISbfISphIXmnIBfuAIBspMIHindIII2,1202,1402,1602,1802,2002,2202,2402,260
ggtagtgtggggtcccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtgcaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagccatcacaccccagggggtacgctctcatcccttgacggtccgtagtttattttgctttccgagtcacgtttctgacccggaaagcaaaatagacaacaaacagccacttgcgagaggactcatcctgtttaggcggccctcgcctaaacttgcaacgcttcgttgccgggcctcKflIPpuMIEcoO109I2,2802,3002,3202,3402,3602,3802,4002,4202,440
ggtggcgggcaggacgcccgccataaactgccaggcatcaaattaagcagaaggccatcctgacggatggcctttttgcgtttctacaaactctttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaccaccgcccgtcctgcgggcggtatttgacggtccgtagtttaattcgtcttccggtaggactgcctaccggaaaaacgcaaagatgtttgagaaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttbla\promoter2,4602,4802,5002,5202,5402,5602,5802,6002,620
aaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaaBssSIBssSαIamp\resistbl...r2,6402,6602,6802,7002,7202,7402,7602,7802,800
gagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatgctctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcacaactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctacScaIXmnIamp\resist2,8202,8402,8602,8802,9002,9202,9402,960
gcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccatacccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggPvuIamp\resist2,9803,0003,0203,0403,0603,0803,1003,1203,140
aaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgcttttgctgctcgcactgtggtgctacggacgtcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgaAseIBglIFspIPstINmeAIIIamp\resist3,1603,1803,2003,2203,2403,2603,2803,3003,320
gataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtctatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacaAhdIBpmIBsaI3,3403,3603,3803,4003,4203,4403,4603,4803,500
cagaccaagtttactcatatatactttagattgatttacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtctggttcaaatgagtatatatgaaatctaactaaatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggNaeINgoMIV3,5203,5403,5603,5803,6003,6203,6403,660
gtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccacagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtAloIBsaAIBsaXIBtgZIDraIII3,6803,7003,7203,7403,7603,7803,8003,8203,840
aactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttaaaaggatctaggtgaagatcctttttgataattgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaattttcctagatccacttctaggaaaaactattPsiI3,8603,8803,9003,9203,9403,9603,9804,0004,020
tctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttga4,0404,0604,0804,1004,1204,1404,1604,1804,200
ctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggtgaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaAlwNI4,2204,2404,2604,2804,3004,3204,3404,360
tggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgtaactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattc4,3804,4004,4204,4404,4604,4804,5004,5204,540
cggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggacBssSIBssSαI4,5604,5804,6004,6204,6404,6604,6804,7004,720
gccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatPciISapIBspQI4,7404,7604,7804,8004,8204,8404,8604,8804,900
cgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcagcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtBsaAIPflFIBstAPIBstZ17ITth111I4,9204,9404,9604,9805,0005,0205,0405,060
tccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagccaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctcggAfeIBtgIPfoIBsmBIEsp3IEcoO109I5,0805,1005,1205,1405,1605,1805,2005,2205,240
cgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatctaattctcatgtttgacagcttatcgctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagattaagagtacaaactgtcgaatagNaeISgrAINgoMIV5,2605,2805,3005,3205,3405,360
  • Plasmid
Editing disabled on read-only sequence.
5371 bp4000425045004750500025050075010001250GFP\in\pGLOPrimer 5 (DNA)BspDI,ClaI,NsiI,BstAPI,BsaBI,AflIIBaeI,EcoRVarabinose\C\gene,AjuIBssHIINruI,BspEIPflMIBspQI,SapIAgeIarabinose\promoter,BstEII,BsmI,MluIBsaXIBarIAlwNIBssSIBssSαIPciI,BspQI,SapIBstAPI,BstZ17I,BsaAI,PflFI,Tth111IPfoI,BsmBI,Esp3I,BtgI,EcoO109I,AfeI,SgrAI,NgoMIV,NaeI

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5371