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pDEST15
  • Sequence Map
    Editing disabled on read-only sequence.
    AtCgAgAtCtCgAtCcCgCgAaAtTaAtAcGaCtCaCtAtAgGgAgAcCaCaAcGgTtTcCcTcTaGaAaTaAtTtTgTtTaAcTtTaAgAaGgAgAtAtAcAtAtGtCcCcTaTaCtAgGtTaTtGgAaAaTtAaGgGcCtTgTgCaAcCcAcTcGaCtTcTtTtGgAaTaTcTTaGcTcTaGaGcTaGgGcGcTtTaAtTaTgCtGaGtGaTaTcCcTcTgGtGtTgCcAaAgGgAgAtCtTtAtTaAaAcAaAtTgAaAtTcTtCcTcTaTaTgTaTaCaGgGgAtAtGaTcCaAtAaCcTtTtAaTtCcCgGaAcAcGtTgGgTgAgCtGaAgAaAaCcTtAtAgAAseIBarINdeIXbaIBglIIEcoNIGST tag (no stop)T7 primerT7 promoterInvitrogen vector 57620406080100120140160
    tGaAgAaAaAtAtGaAgAgCaTtTgTaTgAgCgCgAtGaAgGtGaTaAaTgGcGaAaCaAaAaGtTtGaAtTgGgTtTgGaGtTtCcCaAtCtTcCtTaTtAtAtTgAtGgTgAtGtTaAaTtAaCaCaGtCtAtGgCcAtCaTaCgTtAtAtAgCtGaCaAgCaCaAcAtGtTgaCtTcTtTtTaTaCtTcTcGtAaAcAtAcTcGcGcTaCtTcCaCtAtTtAcCgCtTtGtTtTtCaAaCtTaAcCcAaAcCtCaAaGgGtTaGaAgGaAtAaTaTaAcTaCcAcTaCaAtTtAaTtGtGtCaGaTaCcGgTaGtAtGcAaTaTaTcGaCtGtTcGtGtTgTaCaAcPciISapIBspQIAflIIIGST tag (no stop)Invitrogen vector 576180200220240260280300320340
    GgTgGtTgTcCaAaAgAgCgTgCaGaGaTtTcAaTgCtTgAaGgAgCgGtTtTgGaTaTtAgAtAcGgTgTtTcGaGaAtTgCaTaTaGtAaAgAcTtTgAaAcTcTcAaAgTtGaTtTtCtTaGcAaGcTaCcTgAaAtGcTgAaAaTgTtCgAaGaTcGtTtAtGtCaTaAaACcAcCaAcAgGtTtTcTcGcAcGtCtCtAaAgTtAcGaAcTtCcTcGcCaAaAcCtAtAaTcTaTgCcAcAaAgCtCtTaAcGtAtAtCaTtTcTgAaAcTtTgAgAgTtTcAaCtAaAaGaAtCgTtCgAtGgAcTtTaCgAcTtTtAcAaGcTtCtAgCaAaTaCaGtAtTtTBsgIBstBIGST tag (no stop)Invitrogen vector 576360380400420440460480500520
    cAtAtTtAaAtGgTgAtCaTgTaAcCcAtCcTgAcTtCaTgTtGtAtGaCgCtCtTgAtGtTgTtTtAtAcAtGgAcCcAaTgTgCcTgGaTgCgTtCcCaAaAtTaGtTtGtTtTaAaAaAcGtAtTgAaGcTaTcCcAcAaAtTgAtAaGtAcTtGaAaTcCaGcAaGtAtAtgTaTaAaTtTaCcAcTaGtAcAtTgGgTaGgAcTgAaGtAcAaCaTaCtGcGaGaAcTaCaAcAaAaTaTgTaCcTgGgTtAcAcGgAcCtAcGcAaGgGtTtTaAtCaAaCaAaAtTtTtTgCaTaAcTtCgAtAgGgTgTtTaAcTaTtCaTgAaCtTtAgGtCgTtCaTaTaBclISwaIGST tag (no stop)Invitrogen vector 576540560580600620640660680700
    AgCaTgGcCtTtGcAgGgCtGgCaAgCcAcGtTtGgTgGtGgCgAcCaTcCtCcAaAaTcGgAtCtGgTtCcGcGtCcAtGgTcGaAtCaAaCaAgTtTgTaCaAaAaAgCtGaAcGaGaAaCgTaAaAtGaTaTaAaTaTcAaTaTaTtAaAtTaGaTtTtGcAtAaAaAaCaGTcGtAcCgGaAaCgTcCcGaCcGtTcGgTgCaAaCcAcCaCcGcTgGtAgGaGgTtTtAgCcTaGaCcAaGgCgCaGgTaCcAgCtTaGtTtGtTcAaAcAtGtTtTtTcGaCtTgCtCtTtGcAtTtTaCtAtAtTtAtAgTtAtAtAaTtTaAtCtAaAaCgTaTtTtTtGtCNcoIattR1GST tag (no stop)Invitrogen vector 576720740760780800820840860
    aCtAcAtAaTaCtGtAaAaCaCaAcAtAtCcAgTcAcTaTgGcGgCcGcAtTaGgCaCcCcAgGcTtTaCaCtTtAtGcTtCcGgCtCgTaTaAtGtGtGgAtTtTgAgTtAgGaTcCgTcGaGaTtTtCaGgAgCtAaGgAaGcTaAaAtGgAgAaAaAaAtCaCtGgAtAtActGaTgTaTtAtGaCaTtTtGtGtTgTaTaGgTcAgTgAtAcCgCcGgCgTaAtCcGtGgGgTcCgAaAtGtGaAaTaCgAaGgCcGaGcAtAtTaCaCaCcTaAaAcTcAaTcCtAgGcAgCtCtAaAaGtCcTcGaTtCcTtCgAtTtTaCcTcTtTtTtTaGtGaCcTaTaTgEagINotIattR1Cm(R)Invitrogen vector 5768809009209409609801,0001,0201,040
    CaCcGtTgAtAtAtCcCaAtGgCaTcGtAaAgAaCaTtTtGaGgCaTtTcAgTcAgTtGcTcAaTgTaCcTaTaAcCaGaCcGtTcAgCtGgAtAtTaCgGcCtTtTtAaAgAcCgTaAaGaAaAaTaAgCaCaAgTtTtAtCcGgCcTtTaTtCaCaTtCtTgCcCgCcTgAtGGtGgCaAcTaTaTaGgGtTaCcGtAgCaTtTcTtGtAaAaCtCcGtAaAgTcAgTcAaCgAgTtAcAtGgAtAtTgGtCtGgCaAgTcGaCcTaTaAtGcCgGaAaAaTtTcTgGcAtTtCtTtTtAtTcGtGtTcAaAaTaGgCcGgAaAtAaGtGtAaGaAcGgGcGgAcTaCPvuIICm(R)Invitrogen vector 5761,0601,0801,1001,1201,1401,1601,1801,2001,220
    aAtGcTcAtCcGgAaTtCcGtAtGgCaAtGaAaGaCgGtGaGcTgGtGaTaTgGgAtAgTgTtCaCcCtTgTtAcAcCgTtTtCcAtGaGcAaAcTgAaAcGtTtTcAtCgCtCtGgAgTgAaTaCcAcGaCgAtTtCcGgCaGtTtCtAcAcAtAtAtTcGcAaGaTgTgGcGttTaCgAgTaGgCcTtAaGgCaTaCcGtTaCtTtCtGcCaCtCgAcCaCtAtAcCcTaTcAcAaGtGgGaAcAaTgTgGcAaAaGgTaCtCgTtTgAcTtTgCaAaAgTaGcGaGaCcTcAcTtAtGgTgCtGcTaAaGgCcGtCaAaGaTgTgTaTaTaAgCgTtCtAcAcCgCaApoIEcoRICm(R)Invitrogen vector 5761,2401,2601,2801,3001,3201,3401,3601,3801,400
    GtTaCgGtGaAaAcCtGgCcTaTtTcCcTaAaGgGtTtAtTgAgAaTaTgTtTtTcGtCtCaGcCaAtCcCtGgGtGaGtTtCaCcAgTtTtGaTtTaAaCgTgGcCaAtAtGgAcAaCtTcTtCgCcCcCgTtTtCaCcAtGgGcAaAtAtTaTaCgCaAgGcGaCaAgGtGcTCaAtGcCaCtTtTgGaCcGgAtAaAgGgAtTtCcCaAaTaAcTcTtAtAcAaAaAgCaGaGtCgGtTaGgGaCcCaCtCaAaGtGgTcAaAaCtAaAtTtGcAcCgGtTaTaCcTgTtGaAgAaGcGgGgGcAaAaGtGgTaCcCgTtTaTaAtAtGcGtTcCgCtGtTcCaCgANcoIPasISspIBsmBIEsp3ICm(R)Invitrogen vector 5761,4201,4401,4601,4801,5001,5201,5401,560
    gAtGcCgCtGgCgAtTcAgGtTcAtCaTgCcGtCtGtGaTgGcTtCcAtGtCgGcAgAaTgCtTaAtGaAtTaCaAcAgTaCtGcGaTgAgTgGcAgGgCgGgGcGtAaTcTaGaGgAtCcGgCtTaCtAaAaGcCaGaTaAcAgTaTgCgTaTtTgCgCgCtGaTtTtTgCgGtcTaCgGcGaCcGcTaAgTcCaAgTaGtAcGgCaGaCaCtAcCgAaGgTaCaGcCgTcTtAcGaAtTaCtTaAtGtTgTcAtGaCgCtAcTcAcCgTcCcGcCcCgCaTtAgAtCtCcTaGgCcGaAtGaTtTtCgGtCtAtTgTcAtAcGcAtAaAcGcGcGaCtAaAaAcGcCaXbaIBssHIICm(R)Invitrogen vector 5761,5801,6001,6201,6401,6601,6801,7001,7201,740
    AtAaGaAtAtAtAcTgAtAtGtAtAcCcGaAgTaTgTcAaAaAgAgGtGtGcTaTgAaGcAgCgTaTtAcAgTgAcAgTtGaCaGcGaCaGcTaTcAgTtGcTcAaGgCaTaTaTgAtGtCaAtAtCtCcGgTcTgGtAaGcAcAaCcAtGcAgAaTgAaGcCcGtCgTcTgCgTTaTtCtTaTaTaTgAcTaTaCaTaTgGgCtTcAtAcAgTtTtTcTcCaCaCgAtAcTtCgTcGcAtAaTgTcAcTgTcAaCtGtCgCtGtCgAtAgTcAaCgAgTtCcGtAtAtAcTaCaGtTaTaGaGgCcAgAcCaTtCgTgTtGgTaCgTcTtAcTtCgGgCaGcAgAcGcABstZ17IInvitrogen vector 5761,7601,7801,8001,8201,8401,8601,8801,9001,920
    gCcGaAcGcTgGaAaGcGgAaAaTcAgGaAgGgAtGgCtGaGgTcGcCcGgTtTaTtGaAaTgAaCgGcTcTtTtGcTgAcGaGaAcAgGgAcTgGtGaAaTgCaGtTtAaGgTtTaCaCcTaTaAaAgAgAgAgCcGtTaTcGtCtGtTtGtGgAtGtAcAgAgTgAtAtTaTtcGgCtTgCgAcCtTtCgCcTtTtAgTcCtTcCcTaCcGaCtCcAgCgGgCcAaAtAaCtTtAcTtGcCgAgAaAaCgAcTgCtCtTgTcCcTgAcCaCtTtAcGtCaAaTtCcAaAtGtGgAtAtTtTcTcTcTcGgCaAtAgCaGaCaAaCaCcTaCaTgTcTcAcTaTaAtAaAlwNIBbvCIccdBInvitrogen vector 5761,9401,9601,9802,0002,0202,0402,0602,0802,100
    GaCaCgCcCgGgCgAcGgAtGgTgAtCcCcCtGgCcAgTgCaCgTcTgCtGtCaGaTaAaGtCtCcCgTgAaCtTtAcCcGgTgGtGcAtAtCgGgGaTgAaAgCtGgCgCaTgAtGaCcAcCgAtAtGgCcAgTgTgCcGgTcTcCgTtAtCgGgGaAgAaGtGgCtGaTcTcACtGtGcGgGcCcGcTgCcTaCcAcTaGgGgGaCcGgTcAcGtGcAgAcGaCaGtCtAtTtCaGaGgGcAcTtGaAaTgGgCcAcCaCgTaTaGcCcCtAcTtTcGaCcGcGtAcTaCtGgTgGcTaTaCcGgTcAcAcGgCcAgAgGcAaTaGcCcCtTcTtCaCcGaCtAgAgTSmaISrfIXmaIBmgBIBstXITspMIccdBInvitrogen vector 5762,1202,1402,1602,1802,2002,2202,2402,260
    gCcAcCgCgAaAaTgAcAtCaAaAaCgCcAtTaAcCtGaTgTtCtGgGgAaTaTaAaTgTcAgGcTcCcTtAtAcAcAgCcAgTcTgCaGgTcGaCcAtAgTgAcTgGaTaTgTtGtGtTtTaCaGtAtTaTgTaGtCtGtTtTtTaTgCaAaAtCtAaTtTaAtAtAtTgAtAtcGgTgGcGcTtTtAcTgTaGtTtTtGcGgTaAtTgGaCtAcAaGaCcCcTtAtAtTtAcAgTcCgAgGgAaTaTgTgTcGgTcAgAcGtCcAgCtGgTaTcAcTgAcCtAtAcAaCaCaAaAtGtCaTaAtAcAtCaGaCaAaAaAtAcGtTtTaGaTtAaAtTaTaTaAcTaTaPstISalIBfuAIBspMIattR2ccdBInvitrogen vector 5762,2802,3002,3202,3402,3602,3802,4002,4202,440
    TtAtAtCaTtTtAcGtTtCtCgTtCaGcTtTcTtGtAcAaAgTgGtTtGaTtCgAcCcGgGaTcCgGcTgCtAaCaAaGcCcGaAaGgAaGcTgAgTtGgCtGcTgCcAcCgCtGaGcAaTaAcTaGcAtAaCcCcTtGgGgCcTcTaAaCgGgTcTtGaGgGgTtTtTtGcTgAAaTaTaGtAaAaTgCaAaGaGcAaGtCgAaAgAaCaTgTtTcAcCaAaCtAaGcTgGgCcCtAgGcCgAcGaTtGtTtCgGgCtTtCcTtCgAcTcAaCcGaCgAcGgTgGcGaCtCgTtAtTgAtCgTaTtGgGgAaCcCcGgAgAtTtGcCcAgAaCtCcCcAaAaAaCgAcTBlpISmaIXmaITspMIT7 transcription terminatorattR2T7 reve...primerInvitrogen vector 5762,4602,4802,5002,5202,5402,5602,5802,6002,620
    aAgGaGgAaCtAtAtCcGgAtAtCcAcAgGaCgGgTgTgGtCgCcAtGaTcGcGtAgTcGaTaGtGgCtCcAaGtAgCgAaGcGaGcAgGaCtGgGcGgCgGcCaAaGcGgTcGgAcAgTgCtCcGaGaAcGgGtGcGcAtAgAaAtTgCaTcAaCgCaTaTaGcGcTaGcAgCatTcCtCcTtGaTaTaGgCcTaTaGgTgTcCtGcCcAcAcCaGcGgTaCtAgCgCaTcAgCtAtCaCcGaGgTtCaTcGcTtCgCtCgTcCtGaCcCgCcGcCgGtTtCgCcAgCcTgTcAcGaGgCtCtTgCcCaCgCgTaTcTtTaAcGtAgTtGcGtAtAtCgCgAtCgTcGtBmtINheIEcoRVFspAIT7 tran...inatorInvitrogen vector 5762,6402,6602,6802,7002,7202,7402,7602,7802,800
    CgCcAtAgTgAcTgGcGaTgCtGtCgGaAtGgAcGaTaTcCcGcAaGaGgCcCgGcAgTaCcGgCaTaAcCaAgCcTaTgCcTaCaGcAtCcAgGgTgAcGgTgCcGaGgAtGaCgAtGaGcGcAtTgTtAgAtTtCaTaCaCgGtGcCtGaCtGcGtTaGcAaTtTaAcTgTgAGcGgTaTcAcTgAcCgCtAcGaCaGcCtTaCcTgCtAtAgGgCgTtCtCcGgGcCgTcAtGgCcGtAtTgGtTcGgAtAcGgAtGtCgTaGgTcCcAcTgCcAcGgCtCcTaCtGcTaCtCgCgTaAcAaTcTaAaGtAtGtGcCaCgGaCtGaCgCaAtCgTtAaAtTgAcAcTEcoRVInvitrogen vector 5762,8202,8402,8602,8802,9002,9202,9402,960
    tAaAcTaCcGcAtTaAaGcTtAtCgAtGaTaAgCtGtCaAaCaTgAgAaTtCtTgAaGaCgAaAgGgCcTcGtGaTaCgCcTaTtTtTaTaGgTtAaTgTcAtGaTaAtAaTgGtTtCtTaGaCgTcAgGtGgCaCtTtTcGgGgAaAtGtGcGcGgAaCcCcTaTtTgTtTaTtaTtTgAtGgCgTaAtTtCgAaTaGcTaCtAtTcGaCaGtTtGtAcTcTtAaGaAcTtCtGcTtTcCcGgAgCaCtAtGcGgAtAaAaAtAtCcAaTtAcAgTaCtAtTaTtAcCaAaGaAtCtGcAgTcCaCcGtGaAaAgCcCcTtTaCaCgCgCcTtGgGgAtAaAcAaAtAaApoIClaIZraIAatIIBspDIEcoRIHindIIIbla promoterInvitrogen vector 5762,9803,0003,0203,0403,0603,0803,1003,1203,140
    TtTcTaAaTaCaTtCaAaTaTgTaTcCgCtCaTgAgAcAaTaAcCcTgAtAaAtGcTtCaAtAaTaTtGaAaAaGgAaGaGtAtGaGtAtTcAaCaTtTcCgTgTcGcCcTtAtTcCcTtTtTtGcGgCaTtTtGcCtTcCtGtTtTtGcTcAcCcAgAaAcGcTgGtGaAaGtAAaAgAtTtAtGtAaGtTtAtAcAtAgGcGaGtAcTcTgTtAtTgGgAcTaTtTaCgAaGtTaTtAtAaCtTtTtCcTtCtCaTaCtCaTaAgTtGtAaAgGcAcAgCgGgAaTaAgGgAaAaAaCgCcGtAaAaCgGaAgGaCaAaAaCgAgTgGgTcTtTgCgAcCaCtTtCaTSspIBciVIAmp(R)bla promoterInvitrogen vector 5763,1603,1803,2003,2203,2403,2603,2803,3003,320
    aAaGaTgCtGaAgAtCaGtTgGgTgCaCgAgTgGgTtAcAtCgAaCtGgAtCtCaAcAgCgGtAaGaTcCtTgAgAgTtTtCgCcCcGaAgAaCgTtTtCcAaTgAtGaGcAcTtTtAaAgTtCtGcTaTgTgGcGcGgTaTtAtCcCgTgTtGaCgCcGgGcAaGaGcAaCtCgtTtCtAcGaCtTcTaGtCaAcCcAcGtGcTcAcCcAaTgTaGcTtGaCcTaGaGtTgTcGcCaTtCtAgGaAcTcTcAaAaGcGgGgCtTcTtGcAaAaGgTtAcTaCtCgTgAaAaTtTcAaGaCgAtAcAcCgCgCcAtAaTaGgGcAcAaCtGcGgCcCgTtCtCgTtGaGcAcuIAmp(R)Invitrogen vector 5763,3403,3603,3803,4003,4203,4403,4603,4803,500
    GtCgCcGcAtAcAcTaTtCtCaGaAtGaCtTgGtTgAgTaCtCaCcAgTcAcAgAaAaGcAtCtTaCgGaTgGcAtGaCaGtAaGaGaAtTaTgCaGtGcTgCcAtAaCcAtGaGtGaTaAcAcTgCgGcCaAcTtAcTtCtGaCaAcGaTcGgAgGaCcGaAgGaGcTaAcCgCCaGcGgCgTaTgTgAtAaGaGtCtTaCtGaAcCaAcTcAtGaGtGgTcAgTgTcTtTtCgTaGaAtGcCtAcCgTaCtGtCaTtCtCtTaAtAcGtCaCgAcGgTaTtGgTaCtCaCtAtTgTgAcGcCgGtTgAaTgAaGaCtGtTgCtAgCcTcCtGgCtTcCtCgAtTgGcGBtsIBtsIPvuIBtsαIBtsαIAmp(R)Invitrogen vector 5763,5203,5403,5603,5803,6003,6203,6403,660
    tTtTtTgCaCaAcAtGgGgGaTcAtGtAaCtCgCcTtGaTcGtTgGgAaCcGgAgCtGaAtGaAgCcAtAcCaAaCgAcGaGcGtGaCaCcAcGaTgCcTgCaGcAaTgGcAaCaAcGtTgCgCaAaCtAtTaAcTgGcGaAcTaCtTaCtCtAgCtTcCcGgCaAcAaTtAaTaaAaAaAcGtGtTgTaCcCcCtAgTaCaTtGaGcGgAaCtAgCaAcCcTtGgCcTcGaCtTaCtTcGgTaTgGtTtGcTgCtCgCaCtGtGgTgCtAcGgAcGtCgTtAcCgTtGtTgCaAcGcGtTtGaTaAtTgAcCgCtTgAtGaAtGaGaTcGaAgGgCcGtTgTtAaTtAtAseIPstIAmp(R)Invitrogen vector 5763,6803,7003,7203,7403,7603,7803,8003,8203,840
    GaCtGgAtGgAgGcGgAtAaAgTtGcAgGaCcAcTtCtGcGcTcGgCcCtTcCgGcTgGcTgGtTtAtTgCtGaTaAaTcTgGaGcCgGtGaGcGtGgGtCtCgCgGtAtCaTtGcAgCaCtGgGgCcAgAtGgTaAgCcCtCcCgTaTcGtAgTtAtCtAcAcGaCgGgGaGtCCtGaCcTaCcTcCgCcTaTtTcAaCgTcCtGgTgAaGaCgCgAgCcGgGaAgGcCgAcCgAcCaAaTaAcGaCtAtTtAgAcCtCgGcCaCtCgCaCcCaGaGcGcCaTaGtAaCgTcGtGaCcCcGgTcTaCcAtTcGgGaGgGcAtAgCaTcAaTaGaTgTgCtGcCcCtCaGAhdIAmp(R)Invitrogen vector 5763,8603,8803,9003,9203,9403,9603,9804,0004,020
    aGgCaAcTaTgGaTgAaCgAaAtAgAcAgAtCgCtGaGaTaGgTgCcTcAcTgAtTaAgCaTtGgTaAcTgTcAgAcCaAgTtTaCtCaTaTaTaCtTtAgAtTgAtTtAaAaCtTcAtTtTtAaTtTaAaAgGaTcTaGgTgAaGaTcCtTtTtGaTaAtCtCaTgAcCaAaAttCcGtTgAtAcCtAcTtGcTtTaTcTgTcTaGcGaCtCtAtCcAcGgAgTgAcTaAtTcGtAaCcAtTgAcAgTcTgGtTcAaAtGaGtAtAtAtGaAaTcTaAcTaAaTtTtGaAgTaAaAaTtAaAtTtTcCtAgAtCcAcTtCtAgGaAaAaCtAtTaGaGtAcTgGtTtTaAmp(R)Invitrogen vector 5764,0404,0604,0804,1004,1204,1404,1604,1804,200
    CcCtTaAcGtGaGtTtTcGtTcCaCtGaGcGtCaGaCcCcGtAgAaAaGaTcAaAgGaTcTtCtTgAgAtCcTtTtTtTcTgCgCgTaAtCtGcTgCtTgCaAaCaAaAaAaCcAcCgCtAcCaGcGgTgGtTtGtTtGcCgGaTcAaGaGcTaCcAaCtCtTtTtCcGaAgGtAGgGaAtTgCaCtCaAaAgCaAgGtGaCtCgCaGtCtGgGgCaTcTtTtCtAgTtTcCtAgAaGaAcTcTaGgAaAaAaAgAcGcGcAtTaGaCgAcGaAcGtTtGtTtTtTtGgTgGcGaTgGtCgCcAcCaAaCaAaCgGcCtAgTtCtCgAtGgTtGaGaAaAaGgCtTcCaTAcuIpBR322 originInvitrogen vector 5764,2204,2404,2604,2804,3004,3204,3404,360
    aCtGgCtTcAgCaGaGcGcAgAtAcCaAaTaCtGtCcTtCtAgTgTaGcCgTaGtTaGgCcAcCaCtTcAaGaAcTcTgTaGcAcCgCcTaCaTaCcTcGcTcTgCtAaTcCtGtTaCcAgTgGcTgCtGcCaGtGgCgAtAaGtCgTgTcTtAcCgGgTtGgAcTcAaGaCgAttGaCcGaAgTcGtCtCgCgTcTaTgGtTtAtGaCaGgAaGaTcAcAtCgGcAtCaAtCcGgTgGtGaAgTtCtTgAgAcAtCgTgGcGgAtGtAtGgAgCgAgAcGaTtAgGaCaAtGgTcAcCgAcGaCgGtCaCcGcTaTtCaGcAcAgAaTgGcCcAaCcTgAgTtCtGcTaAlwNIpBR322 originInvitrogen vector 5764,3804,4004,4204,4404,4604,4804,5004,5204,540
    AgTtAcCgGaTaAgGcGcAgCgGtCgGgCtGaAcGgGgGgTtCgTgCaCaCaGcCcAgCtTgGaGcGaAcGaCcTaCaCcGaAcTgAgAtAcCtAcAgCgTgAgCtAtGaGaAaGcGcCaCgCtTcCcGaAgGgAgAaAgGcGgAcAgGtAtCcGgTaAgCgGcAgGgTcGgAaCTcAaTgGcCtAtTcCgCgTcGcCaGcCcGaCtTgCcCcCcAaGcAcGtGtGtCgGgTcGaAcCtCgCtTgCtGgAtGtGgCtTgAcTcTaTgGaTgTcGcAcTcGaTaCtCtTtCgCgGtGcGaAgGgCtTcCcTcTtTcCgCcTgTcCaTaGgCcAtTcGcCgTcCcAgCcTtGBciVIBseYIpBR322 originInvitrogen vector 5764,5604,5804,6004,6204,6404,6604,6804,7004,720
    aGgAgAgCgCaCgAgGgAgCtTcCaGgGgGaAaCgCcTgGtAtCtTtAtAgTcCtGtCgGgTtTcGcCaCcTcTgAcTtGaGcGtCgAtTtTtGtGaTgCtCgTcAgGgGgGcGgAgCcTaTgGaAaAaCgCcAgCaAcGcGgCcTtTtTaCgGtTcCtGgCcTtTtGcTgGcCttCcTcTcGcGtGcTcCcTcGaAgGtCcCcCtTtGcGgAcCaTaGaAaTaTcAgGaCaGcCcAaAgCgGtGgAgAcTgAaCtCgCaGcTaAaAaCaCtAcGaGcAgTcCcCcCgCcTcGgAtAcCtTtTtGcGgTcGtTgCgCcGgAaAaAtGcCaAgGaCcGgAaAaCgAcCgGaDrdIpBR322 originInvitrogen vector 5764,7404,7604,7804,8004,8204,8404,8604,8804,900
    TtTgCtCaCaTgTtCtTtCcTgCgTtAtCcCcTgAtTcTgTgGaTaAcCgTaTtAcCgCcTtTgAgTgAgCtGaTaCcGcTcGcCgCaGcCgAaCgAcCgAgCgCaGcGaGtCaGtGaGcGaGgAaGcGgAaGaGcGcCtGaTgCgGtAtTtTcTcCtTaCgCaTcTgTgCgGtAAaAcGaGtGtAcAaGaAaGgAcGcAaTaGgGgAcTaAgAcAcCtAtTgGcAtAaTgGcGgAaAcTcAcTcGaCtAtGgCgAgCgGcGtCgGcTtGcTgGcTcGcGtCgCtCaGtCaCtCgCtCcTtCgCcTtCtCgCgGaCtAcGcCaTaAaAgAgGaAtGcGtAgAcAcGcCaTPciISapIBspQIAflIIIpB...nInvitrogen vector 5764,9204,9404,9604,9805,0005,0205,0405,060
    tTtCaCaCcGcAtAtAtGgTgCaCtCtCaGtAcAaTcTgCtCtGaTgCcGcAtAgTtAaGcCaGtAtAcAcTcCgCtAtCgCtAcGtGaCtGgGtCaTgGcTgCgCcCcGaCaCcCgCcAaCaCcCgCtGaCgCgCcCtGaCgGgCtTgTcTgCtCcCgGcAtCcGcTtAcAgAcaAaGtGtGgCgTaTaTaCcAcGtGaGaGtCaTgTtAgAcGaGaCtAcGgCgTaTcAaTtCgGtCaTaTgTgAgGcGaTaGcGaTgCaCtGaCcCaGtAcCgAcGcGgGgCtGtGgGcGgTtGtGgGcGaCtGcGcGgGaCtGcCcGaAcAgAcGaGgGcCgTaGgCgAaTgTcTgDrdIBsaAIPflFIBstZ17ITth111IInvitrogen vector 5765,0805,1005,1205,1405,1605,1805,2005,2205,240
    AaGcTgTgAcCgTcTcCgGgAgCtGcAtGtGtCaGaGgTtTtCaCcGtCaTcAcCgAaAcGcGcGaGgCaGcTgCgGtAaAgCtCaTcAgCgTgGtCgTgAaGcGaTtCaCaGaTgTcTgCcTgTtCaTcCgCgTcCaGcTcGtTgAgTtTcTcCaGaAgCgTtAaTgTcTgGcTTtCgAcAcTgGcAgAgGcCcTcGaCgTaCaCaGtCtCcAaAaGtGgCaGtAgTgGcTtTgCgCgCtCcGtCgAcGcCaTtTcGaGtAgTcGcAcCaGcAcTtCgCtAaGtGtCtAcAgAcGgAcAaGtAgGcGcAgGtCgAgCaAcTcAaAgAgGtCtTcGcAaTtAcAgAcCgAPfoIBsmBIEsp3IPvuIIROPInvitrogen vector 5765,2605,2805,3005,3205,3405,3605,3805,4005,420
    tCtGaTaAaGcGgGcCaTgTtAaGgGcGgTtTtTtCcTgTtTgGtCaCtGaTgCcTcCgTgTaAgGgGgAtTtCtGtTcAtGgGgGtAaTgAtAcCgAtGaAaCgAgAgAgGaTgCtCaCgAtAcGgGtTaCtGaTgAtGaAcAtGcCcGgTtAcTgGaAcGtTgTgAgGgTaAaaGaCtAtTtCgCcCgGtAcAaTtCcCgCcAaAaAaGgAcAaAcCaGtGaCtAcGgAgGcAcAtTcCcCcTaAaGaCaAgTaCcCcCaTtAcTaTgGcTaCtTtGcTcTcTcCtAcGaGtGcTaTgCcCaAtGaCtAcTaCtTgTaCgGgCcAaTgAcCtTgCaAcAcTcCcAtTtROPInvitrogen vector 5765,4405,4605,4805,5005,5205,5405,5605,5805,600
    CaAcTgGcGgTaTgGaTgCgGcGgGaCcAgAgAaAaAtCaCtCaGgGtCaAtGcCaGcGcTtCgTtAaTaCaGaTgTaGgTgTtCcAcAgGgTaGcCaGcAgCaTcCtGcGaTgCaGaTcCgGaAcAtAaTgGtGcAgGgCgCtGaCtTcCgCgTtTcCaGaCtTtAcGaAaCaCGtTgAcCgCcAtAcCtAcGcCgCcCtGgTcTcTtTtTaGtGaGtCcCaGtTaCgGtCgCgAaGcAaTtAtGtCtAcAtCcAcAaGgTgTcCcAtCgGtCgTcGtAgGaCgCtAcGtCtAgGcCtTgTaTtAcCaCgTcCcGcGaCtGaAgGcGcAaAgGtCtGaAaTgCtTtGtGBsgIInvitrogen vector 5765,6205,6405,6605,6805,7005,7205,7405,760
    gGaAaCcGaAgAcCaTtCaTgTtGtTgCtCaGgTcGcAgAcGtTtTgCaGcAgCaGtCgCtTcAcGtTcGcTcGcGtAtCgGtGaTtCaTtCtGcTaAcCaGtAaGgCaAcCcCgCcAgCcTaGcCgGgTcCtCaAcGaCaGgAgCaCgAtCaTgCgCaCcCgTgGcCaGgAcCccCtTtGgCtTcTgGtAaGtAcAaCaAcGaGtCcAgCgTcTgCaAaAcGtCgTcGtCaGcGaAgTgCaAgCgAgCgCaTaGcCaCtAaGtAaGaCgAtTgGtCaTtCcGtTgGgGcGgTcGgAtCgGcCcAgGaGtTgCtGtCcTcGtGcTaGtAcGcGtGgGcAcCgGtCcTgGgFspAIPpuMIPpuMIInvitrogen vector 5765,7805,8005,8205,8405,8605,8805,9005,9205,940
    AaCgCtGcCcGaGaTgCgCcGcGtGcGgCtGcTgGaGaTgGcGgAcGcGaTgGaTaTgTtCtGcCaAgGgTtGgTtTgCgCaTtCaCaGtTcTcCgCaAgAaTtGaTtGgCtCcAaTtCtTgGaGtGgTgAaTcCgTtAgCgAgGtGcCgCcGgCtTcCaTtCaGgTcGaGgTgGTtGcGaCgGgCtCtAcGcGgCgCaCgCcGaCgAcCtCtAcCgCcTgCgCtAcCtAtAcAaGaCgGtTcCcAaCcAaAcGcGtAaGtGtCaAgAgGcGtTcTtAaCtAaCcGaGgTtAaGaAcCtCaCcAcTtAgGcAaTcGcTcCaCgGcGgCcGaAgGtAaGtCcAgCtCcAcCInvitrogen vector 5765,9605,9806,0006,0206,0406,0606,0806,1006,120
    cCcGgCtCcAtGcAcCgCgAcGcAaCgCgGgGaGgCaGaCaAgGtAtAgGgCgGcGcCtAcAaTcCaTgCcAaCcCgTtCcAtGtGcTcGcCgAgGcGgCaTaAaTcGcCgTgAcGaTcAgCgGtCcAgTgAtCgAaGtTaGgCtGgTaAgAgCcGcGaGcGaTcCtTgAaGcTggGgCcGaGgTaCgTgGcGcTgCgTtGcGcCcCtCcGtCtGtTcCaTaTcCcGcCgCgGaTgTtAgGtAcGgTtGgGcAaGgTaCaCgAgCgGcTcCgCcGtAtTtAgCgGcAcTgCtAgTcGcCaGgTcAcTaGcTtCaAtCcGaCcAtTcTcGgCgCtCgCtAgGaAcTtCgAcInvitrogen vector 5766,1406,1606,1806,2006,2206,2406,2606,2806,300
    TcCcTgAtGgTcGtCaTcTaCcTgCcTgGaCaGcAtGgCcTgCaAcGcGgGcAtCcCgAtGcCgCcGgAaGcGaGaAgAaTcAtAaTgGgGaAgGcCaTcCaGcCtCgCgTcGcGaAcGcCaGcAaGaCgTaGcCcAgCgCgTcGgCcGcCaTgCcGgCgAtAaTgGcCtGcTtCAgGgAcTaCcAgCaGtAgAtGgAcGgAcCtGtCgTaCcGgAcGtTgCgCcCgTaGgGcTaCgGcGgCcTtCgCtCtTcTtAgTaTtAcCcCtTcCgGtAgGtCgGaGcGcAgCgCtTgCgGtCgTtCtGcAtCgGgTcGcGcAgCcGgCgGtAcGgCcGcTaTtAcCgGaCgAaGEagINruIBfuAIBseYIBspMIBstAPIInvitrogen vector 5766,3206,3406,3606,3806,4006,4206,4406,460
    tCgCcGaAaCgTtTgGtGgCgGgAcCaGtGaCgAaGgCtTgAgCgAgGgCgTgCaAgAtTcCgAaTaCcGcAaGcGaCaGgCcGaTcAtCgTcGcGcTcCaGcGaAaGcGgTcCtCgCcGaAaAtGaCcCaGaGcGcTgCcGgCaCcTgTcCtAcGaGtTgCaTgAtAaAgAaGaaGcGgCtTtGcAaAcCaCcGcCcTgGtCaCtGcTtCcGaAcTcGcTcCcGcAcGtTcTaAgGcTtAtGgCgTtCgCtGtCcGgCtAgTaGcAgCgCgAgGtCgCtTtCgCcAgGaGcGgCtTtTaCtGgGtCtCgCgAcGgCcGtGgAcAgGaTgCtCaAcGtAcTaTtTcTtCtInvitrogen vector 5766,4806,5006,5206,5406,5606,5806,6006,6206,640
    CaGtCaTaAgTgCgGcGaCgAtAgTcAtGcCcCgCgCcCaCcGgAaGgAgCtGaCtGgGtTgAaGgCtCtCaAgGgCaTcGgTcGaTcGaCgCtCtCcCtTaTgCgAcTcCtGcAtTaGgAaGcAgCcCaGtAgTaGgTtGaGgCcGtTgAgCaCcGcCgCcGcAaGgAaTgGtGGtCaGtAtTcAcGcCgCtGcTaTcAgTaCgGgGcGcGgGtGgCcTtCcTcGaCtGaCcCaAcTtCcGaGaGtTcCcGtAgCcAgCtAgCtGcGaGaGgGaAtAcGcTgAgGaCgTaAtCcTtCgTcGgGtCaTcAtCcAaCtCcGgCaAcTcGtGgCgGcGgCgTtCcTtAcCaCPvuIEcoNIPshAIInvitrogen vector 5766,6606,6806,7006,7206,7406,7606,7806,8006,820
    cAtGcAaGgAgAtGgCgCcCaAcAgTcCcCcGgCcAcGgGgCcTgCcAcCaTaCcCaCgCcGaAaCaAgCgCtCaTgAgCcCgAaGtGgCgAgCcCgAtCtTcCcCaTcGgTgAtGtCgGcGaTaTaGgCgCcAgCaAcCgCaCcTgTgGcGcCgGtGaTgCcGgCcAcGaTgCggTaCgTtCcTcTaCcGcGgGtTgTcAgGgGgCcGgTgCcCcGgAcGgTgGtAtGgGtGcGgCtTtGtTcGcGaGtAcTcGgGcTtCaCcGcTcGgGcTaGaAgGgGtAgCcAcTaCaGcCgCtAtAtCcGcGgTcGtTgGcGtGgAcAcCgCgGcCaCtAcGgCcGgTgCtAcGcSphIBanIIBanIISgrAIInvitrogen vector 5766,8406,8606,8806,9006,9206,9406,9606,9807,000
    TcCgGcGtAgAgGAgGcCgCaTcTcCInv...767,010
    • Plasmid
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    Editing disabled on read-only sequence.
    7013 bp500055006000650050010001500ROPCm(R)initiation ATGInvitrogen vector 576

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    7013