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pLB260
  • Sequence Map
    Editing disabled on read-only sequence.
    TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCDrdIPfoIBsmBIBsmBIEsp3IEsp3I20406080100120140160
    AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTATCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATBglIKasINarINdeISfoIPluTIPvuIIBstAPILac-operonLacZ alpha180200220240260280300320340
    ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCTCGCGAATGCATCTAGATCCAATTTTCTCTTAAACAACTCCAGACCAGTAAGTTACATGTTTTTTTTTTATTGGAAAGATGTTACAGACTGATGTTTGTAAAAGTGTTTCTTTGCGGTCCCAAAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTTAAGCTCGAGCCATGGAGCGCTTACGTAGATCTAGGTTAAAAGAGAATTTGTTGAGGTCTGGTCATTCAATGTACAAAAAAAAAATAACCTTTCTACAATGTCTGACTACAAACATTTTCACAAAGAAApoIBpmIBsmIKpnINruINsiISacIXbaIEcoRIAcc65IEco53kIPolyA_signalLac-operonM13-fwd360380400420440460480500520
    TTAACCGGTCTTGTACAGCTCGTCCATGCCGTACAGGAACAGGTGGTGGCGGCCCTCGGAGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAGCTTGGTGTCCACGTAGTAGTAGCCGGGCAGTTGCACGGGCTTCTTGGCCATGTAGATGGTCAATTGGCCAGAACATGTCGAGCAGGTACGGCATGTCCTTGTCCACCACCGCCGGGAGCCTCGCGAGCATGACAAGGTGCTACCACATCAGGAGCAACACCCTCCACTACAGGTCGAACCACAGGTGCATCATCATCGGCCCGTCAACGTGCCCGAAGAACCGGTACATCTACCAGAfeIAgeIAleIMscIBsrGIBstXItdTomato540560580600620640660680700
    TTGAACTCCACCAGGTAGTGGCCGCCGTCCTTCAGCTTCAGGGCCTGGTGGATCTCGCCCTTCAGCACGCCGTCGCGGGGGTACAGGCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCGTCGGGGGGGAAGTTGGTGCCGCGCATCTTCACCTTGTAACTTGAGGTGGTCCATCACCGGCGGCAGGAAGTCGAAGTCCCGGACCACCTAGAGCGGGAAGTCGTGCGGCAGCGCCCCCATGTCCGCGAGCCACCTCCGGAGGGTCGGGTACCAGAAGAAGACGTAATGCCCCGGCAGCCCCCCCTTCAACCACGGCGCGTAGAAGTGGAACANcoISexAItdTomato720740760780800820840860
    AGATCAGCGTGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCAGACCGCCGTCCTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCTTGTAATCGGGGATGTCGGCGGGGTGCTTCACGTACGCCTTGGAGCCGTACATGAACTGGGGGGATCTAGTCGCACGGCAGGACGTCCCTCCTCAGGACCCAGTGCCAGTGGTCTGGCGGCAGGAGCTTCAAGTAGTGCGCGAGGGTGAACTTCGGGAGCCCCTTCCTGTCGAAGAACATTAGCCCCTACAGCCGCCCCACGAAGTGCATGCGGAACCTCGGCATGTACTTGACCCCCCTSbfIBsiWIPshAItdTomato8809009209409609801,0001,0201,040
    CAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCATGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCTTTGATGACGGCCATGTTGGTCCTACAGGGTCCGCTTCCCGTCCCCCGGCGGGAACCAGTGGAAGTCGAACCGCCAGACCCACGGGAGCATCCCCGCCGGGAGCGGGAGCGGGAGCTAGAGCTTGAGCACCGGCAAGTACCTCGGGAGGTACGCGTGGAACTTCGCGTACTTGAGAAACTACTGCCGGTACAACFspAItdTomato1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TTGTCCTCGGAGGAGGCGGTGCCGGAGCTGCCGCTGCCGGTGCTGCCGGTGCCATGCCCCAGGAACAGGTGGTGGCGGCCCTCGGAGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAGCTTGGTGTCCACGTAGTAGTAGCCGGGCAGTTGCAAACAGGAGCCTCCTCCGCCACGGCCTCGACGGCGACGGCCACGACGGCCACGGTACGGGGTCCTTGTCCACCACCGCCGGGAGCCTCGCGAGCATGACAAGGTGCTACCACATCAGGAGCAACACCCTCCACTACAGGTCGAACCACAGGTGCATCATCATCGGCCCGTCAACGTAfeIAleItdTomato1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CGGGCTTCTTGGCCATGTAGATGGTCTTGAACTCCACCAGGTAGTGGCCGCCGTCCTTCAGCTTCAGGGCCTGGTGGATCTCGCCCTTCAGCACGCCGTCGCGGGGGTACAGGCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCGTCGGGGGGGAAGCCCGAAGAACCGGTACATCTACCAGAACTTGAGGTGGTCCATCACCGGCGGCAGGAAGTCGAAGTCCCGGACCACCTAGAGCGGGAAGTCGTGCGGCAGCGCCCCCATGTCCGCGAGCCACCTCCGGAGGGTCGGGTACCAGAAGAAGACGTAATGCCCCGGCAGCCCCCCCTTMscINcoIBstXISexAItdTomato1,4201,4401,4601,4801,5001,5201,5401,560
    GTTGGTGCCGCGCATCTTCACCTTGTAGATCAGCGTGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCAGACCGCCGTCCTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCTTGTAATCGGGGATGTCGGCGGGGTGCTTCACGTACGCCCAACCACGGCGCGTAGAAGTGGAACATCTAGTCGCACGGCAGGACGTCCCTCCTCAGGACCCAGTGCCAGTGGTCTGGCGGCAGGAGCTTCAAGTAGTGCGCGAGGGTGAACTTCGGGAGCCCCTTCCTGTCGAAGAACATTAGCCCCTACAGCCGCCCCACGAAGTGCATGCGGSbfIBsiWIPshAItdTomato1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TTGGAGCCGTACATGAACTGGGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCATGGAGCCCTCCATGCGCACCTTGAAGCGCAAACCTCGGCATGTACTTGACCCCCCTGTCCTACAGGGTCCGCTTCCCGTCCCCCGGCGGGAACCAGTGGAAGTCGAACCGCCAGACCCACGGGAGCATCCCCGCCGGGAGCGGGAGCGGGAGCTAGAGCTTGAGCACCGGCAAGTACCTCGGGAGGTACGCGTGGAACTTCGCGTFspAItdTomato1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TGAACTCTTTGATGACCTCCTCGCCCTTGCTCACTAGGCGCGATCGCAAAGCGTAATCTGGAACGTCATATGGATAGGAACCTGCATAGTCCGGGACGTCATAGGGATAGCCAGCGTAATCTGGAACATCGTATGGGTAATGGCGCGTCCGCGGAATCTTTTGGTCCTCATCATTACTTGAGAAACTACTGGAGGAGCGGGAACGAGTGATCCGCGCTAGCGTTTCGCATTAGACCTTGCAGTATACCTATCCTTGGACGTATCAGGCCCTGCAGTATCCCTATCGGTCGCATTAGACCTTGTAGCATACCCATTACCGCGCAGGCGCCTTAGAAAACCAGGAGTAGTAANdeIPfoIZraIAatIIAsiSIBfuAIBspMISacII3xHAtdTomatoPalmy1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTTTTCAACCTGTTTGGTTCTTCTCATACAGCACAGATGGCGCGCCATTTTTTAAGTCTTCCAAGAGACACGAAAACGTGTGCACACCAGAAAATTTCTCAAACAGAAAGAAAGATTTGGGGGCCACAGCTTCCTGAACATGTGGGTTTGAGACACCAAAATAATTTCCACCTAAGAAAAGTTGGACAAACCAAGAAGAGTATGTCGTGTCTACCGCGCGGTAAAAAATTCAGAAGGTTCTCTGTGCTTTTGCACACGTGTGGTCTTTTAAAGAGTTTGTCTTTCTTTCTAAACCCCCGGTGTCGAAGGACTTGTACACCCAAACTCTGTGGTTTTATTAAAGGTGGATTApoIAscIBssHIIRPP2Palmy2,1202,1402,1602,1802,2002,2202,2402,260
    ATTATTAACAATTTGTTTATAAATAGCCCTGTAAACATCAAATCTCCCTATAGTGAGTCGTATTAGGATCCCGGGCCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACTAATAATTGTTAAACAAATATTTATCGGGACATTTGTAGTTTAGAGGGATATCACTCAGCATAATCCTAGGGCCCGGGCAGCTGACGTCTCCGGACGTACGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGAccIApaIPsiISalISmaISphIXmaIBamHITspMIHincIIPspOMIHindIIIRPP2T7LacOM13-revT72,2802,3002,3202,3402,3602,3802,4002,4202,440
    GAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGSapIBspQIPvuII2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGColE1 origin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTDrdIMmeIBciVIColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)2,8202,8402,8602,8802,9002,9202,9402,960
    GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGMmeIColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGAColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTCCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAAColE1 originpMB1 origin of replication ...5 to RNA/DNA switch point)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCTTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGAmpR3,5203,5403,5603,5803,6003,6203,6403,660
    CAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACBglIBpmIBsaIBsrDINmeAIIIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCAclIBsrDIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCBcgIScaIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCATTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTAclIXmnIAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    AAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCSspIZraIBciVI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGAatII4,5604,5804,600
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4616 bp325035003750400042502505007501000tdTomatoM13-fwdLB386 (DNA)LB388 (DNA)LB385 (DNA)BsmBI,Esp3I,BsmBI,Esp3I,PfoI,DrdI,NdeI,BstAPI,KasI,NarI,SfoI,PluTILac-operon,LacZ alpha,BglI,PvuII,ApoI,EcoRI,Eco53kI,SacI,Acc65IPolyA_signal,KpnI,NruI,BsmI,NsiI,XbaI,BpmIAgeI,BsrGI,AfeI,AleIMscIBstXI,SexAINcoISbfIPshAIBsiWIBsaI,BsrDI,BpmIBglI,NmeAIIIAclIBsrDIAmpRScaI,BcgIAclI,XmnISspI,BciVI,ZraI,AatII

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    4616