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PUC57-T7-RPP2(5UTR)-GCaMP6s(AscI-AgeI)_c1
  • Sequence Map
    Editing disabled on read-only sequence.
    TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCDrdIPfoIBsmBIBsmBIEsp3IEsp3IpBR322 [Split]20406080100120140160
    AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTATCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATBglIFspIKasINarINdeIPvuISfoIPluTIPvuIIBstAPILac-operonLacZ alphapBR322 [Split]180200220240260280300320340
    ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCTCGCGAATGCATCTAGATCCAATTTTCTCTTAAACAACTCCAGACCAGTAAGTTACATGTTTTTTTTTTATTGGAAAGATGTTACAGACTGATGTTTGTAAAAGTGTTTCTTTGCGGTCCCAAAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTTAAGCTCGAGCCATGGAGCGCTTACGTAGATCTAGGTTAAAAGAGAATTTGTTGAGGTCTGGTCATTCAATGTACAAAAAAAAAATAACCTTTCTACAATGTCTGACTACAAACATTTTCACAAAGAABmrIBsmIKpnINruINsiISacIXbaIEcoRIAcc65IEco53kIPolyA_signalLac-operonM13-fwd360380400420440460480500520
    TTAACCGGTCTTCGCTGTCATCATTTGTACAAACTCTTCGTAGTTTACCTGACCATCCCCATCGATGTCTGCTTCCCTGATCATTTCATCAACCTCTTCATCTGTTAACTTCTCTCCAAGGTTTGTCATCACGTGGCGAAGCTCTGCTGCACTGATGTAGCCATTGCCATCCTTAAATTGGCCAGAAGCGACAGTAGTAAACATGTTTGAGAAGCATCAAATGGACTGGTAGGGGTAGCTACAGACGAAGGGACTAGTAAAGTAGTTGGAGAAGTAGACAATTGAAGAGAGGTTCCAAACAGTAGTGCACCGCTTCGAGACGACGTGACTACATCGGTAACGGTAGGAATAgeIBarIBbsIBclIClaIHpaIPmlIBsaAIBspDIBsrGIGCaMP6s540560580600620640660680700
    TCAAACACACCGAACGCTTCTCTAATTTCTTCTTCCGTGTCCCTGTATTTCATTTTTCTTGCCATCATTGTCAGGAACTCAGGGAAGTCGATTGTGCCGTCACCGTCGGCATCTACTTCATTGATCATGTCCTGCAGCTCTGCTTCTGTGGGGTTCTGCCCCAGAGACCGCATCAAGTTTGTGTGGCTTGCGAAGAGATTAAAGAAGAAGGCACAGGGACATAAAGTAAAAAGAACGGTAGTAACAGTCCTTGAGTCCCTTCAGCTAACACGGCAGTGGCAGCCGTAGATGAAGTAACTAGTACAGGACGTCGAGACGAAGACACCCCAAGACGGGGTCTCTGGCGTAGTBclIBsaIPstIGCaMP6s720740760780800820840860
    CCGTCCCCAGCTCCTTGGTTGTTATTGTCCCATCCCCGTCCTTGTCAAATAGGGAGAAAGCCTCTTTAAATTCTGCGATCTGCTCTTCAGTCAGTTGGTCCGGCAGGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGGCAGGGGTCGAGGAACCAACAATAACAGGGTAGGGGCAGGAACAGTTTATCCCTCTTTCGGAGAAATTTAAGACGCTAGACGAGAAGTCAGTCAACCAGGCCGTCCAACATGAGGTCGAACACGGGGTCCTACAACGGCAGGAGGAACTTCAGCTACGGGAAGTCGAGCTACGCBplIBplISapIBfuAIBseYIBspMIBspQIBstXIGCaMP6s8809009209409609801,0001,0201,040
    GTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGATGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGCAAGTGGTCCCACAGCGGGAGCTTGAAGTGGAGCCGCGCCCAGAACATCAACGGCAGCAGGAACTTCTTCTACCACGCGAGGACCTACATCGGAAGCCCGTACCGCCTGAACTTCTTCAGCACGACGAAGTACACCAGCCCCATCGCCGACTTCGTGACGTGCGGCATCCAGTCCPfoIGCaMP6s1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCACCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCACCAGTGCTCCCACCCGGTCCCGTGCCCGTCGAACGGCCACCACGTCTACTTGAAGTCCCAGTCGAACGGCATCCACCGTAGCGGGAGTGGGAGCGGCCTGTGCGACTTGAACACCGGCAAATGCAGCGGCAGGTCGAGCTGGTCCTACCCGTGGTGGGGCCACTTGTCGAGGABseRIBtgZIGCaMP6s1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CGCCCTTGCTCACCATGCTCCCTCCGGTACCGCCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTCGAAAGTTTGGACTGCACGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCGGGAACGAGTGGTACGAGGGAGGCCATGGCGGGAACATGTCGAGCAGGTACGGCTCTCACTAGGGCCGCCGCCAGTGCTTGAGGTCGTCCTGGTACACTAGCGCGAAGAGCAACCCCAGAAAGCTTTCAAACCTGACGTGCGAGTCCATCACCAACAGCCCGTCGTCGTGCCCKpnIBsrGIBstBIAcc65IBpu10IGCaMP6s1,4201,4401,4601,4801,5001,5201,5401,560
    GCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTAGGCGAGCTGCACGCCGCCGTCCTCGATGTTGTGGCGGATGTGGAAGTTCGCCTTGATGCCGTTCTTCTGCTTGTCGGCCTTGATATAGACGTTCTCGAGTGAGCTCAGCCGACCTATAGCTCTGACTGCGTGACCTGTCCGGCAGCGGCTACCCCCACAAGACGACCATCACCATCCGCTCGACGTGCGGCGGCAGGAGCTACAACACCGCCTACACCTTCAAGCGGAACTACGGCAAGAAGACGAACAGCCGGAACTATATCTGCAAGAGCTCACTCGAGTCGGCTGGATATCGAGACTGACGCACTGGACAGAvaIBlpISacITliIXhoIBsoBIPaeR7IEco53kIGCaMP6s1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TTATTCCACTTACGACGTGATGAGTCGACCATGGTGGCGAGATCCTTATCGTCATCGTCGTACAGATCCCGACCCATTTGCTGTCCACCAGTCATGCTAGCCATACCATGATGATGATGATGATGAGAACCTCGGCGCGCCATTTTTTAAGTCTTCCAAGAGACACGAAAACGTGAATAAGGTGAATGCTGCACTACTCAGCTGGTACCACCGCTCTAGGAATAGCAGTAGCAGCATGTCTAGGGCTGGGTAAACGACAGGTGGTCAGTACGATCGGTATGGTACTACTACTACTACTACTCTTGGAGCCGCGCGGTAAAAAATTCAGAAGGTTCTCTGTGCTTTTGCACAccIAscIBbsIBcgIBmtIBtgINcoINheISalIBmgBIBsaBIBssHIIGCaMP6sRPP21,7601,7801,8001,8201,8401,8601,8801,9001,920
    TGCACACCAGAAAATTTCTCAAACAGAAAGAAAGATTTGGGGGCCACAGCTTCCTGAACATGTGGGTTTGAGACACCAAAATAATTTCCACCTAAATTATTAACAATTTGTTTATAAATAGCCCTGTAAACATCAAATCTCCCTATAGTGAGTCGTATTAGGATCCCGGGCCCGTACGTGTGGTCTTTTAAAGAGTTTGTCTTTCTTTCTAAACCCCCGGTGTCGAAGGACTTGTACACCCAAACTCTGTGGTTTTATTAAAGGTGGATTTAATAATTGTTAAACAAATATTTATCGGGACATTTGTAGTTTAGAGGGATATCACTCAGCATAATCCTAGGGCCCGGGCAApaIAvaIPsiISalISmaIXmaIBamHIBsoBITspMIPspOMIRPP2T7T71,9401,9601,9802,0002,0202,0402,0602,0802,100
    CGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTGACGTCTCCGGACGTACGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGAccIPstISphIStuIHindIIIlac promoterLacOM13-rev2,1202,1402,1602,1802,2002,2202,2402,260
    GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATASapITfiIBsaXIBspQIPvuII2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCDrdITfiIColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGMmeIBciVIBseYIColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTMmeIAlwNIColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)2,8202,8402,8602,8802,9002,9202,9402,960
    CTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGColE1 originpMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCAATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTColE1 originAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCAhdIBglIBmrIBsaIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCATCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTAclIFspIAmpR3,5203,5403,5603,5803,6003,6203,6403,660
    ACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCAPvuIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAAclIBcgIScaIXmnIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCSspI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    TTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGZraIAatIIBciVIEcoO109I4,2204,2404,2604,2804,3004,3204,3404,360
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4361 bp32503500375040002505007501000GCaMP6sM13-fwdLB386 (DNA)LB385 (DNA)

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    4361