Haynes Labhayneslab

Arizona State UniversityJoined in August 2013
modified Dec 15 at 06:22pm
created Oct 20, 2015

hayneslab / Chromatin CRISPR Interference

modified Jul 07 at 10:15pm
created Aug 04, 2013

hayneslab / Mammalian Basic Parts

Parts used to assemble composites

modified Dec 15 at 07:08pm
created Sep 09, 2014

hayneslab / MicroRNA sensors

Customizable two-state genetic switches, where Red indicates the absence of a microRNA and Cyan indicates the presence of a microRNA. Haynes, K. A., Ceroni, F., Flicker, D., Younger, A., & Silver, P. A. (2012). A sensitive switch for visualizing natural gene silencing in single cells. ACS synthetic biology, 1(3), 99–106. doi:10.1021/sb3000035

modified Apr 07 at 11:21pm
created May 29, 2014

hayneslab / miscellaneous

modified Jun 25 at 08:22pm
created Dec 08, 2015

hayneslab / Protocols - Haynes Lab

Shared protocols for the Haynes lab at Arizona State University

modified Jul 14 at 07:16pm
created Jan 30, 2015

hayneslab / SB.ASU

Synthetic Biology plasmids distributed through DNASU.

modified Feb 27 at 01:14am
created Feb 26, 2015

hayneslab / Spark Open Research Synthetic Biology

modified Jul 15 at 05:56pm
created Apr 03, 2014

hayneslab / Synthetic Chromatin Actuators

This unique class of transcription factors (gene activators) are fusion proteins that bind to histone marks (H3K27me3) instead of DNA sequences. The first generation of these actuators, called Pc-TFs, was published by Haynes and Silver in 2011. All proteins listed here are tagged with the mCherry red fluorescent protein for easy visualization, tagged with the myc epitope for biochemical analysis, and placed under control of a Tet repressible (dox-inducible) promoter.
Haynes, K. A., & Silver, P. A. (2011). Synthetic reversal of epigenetic silencing. The Journal of biological chemistry, 286(31), 27176–27182. doi:10.1074/jbc.C111.229567

modified Jun 11 at 02:26am
created Aug 04, 2013

hayneslab / Vectors for Mammalian Expression

DNA plasmid vectors for expression of synthetic genes in mammalian cells.