Haynes Labhayneslab

Arizona State UniversityJoined in August 2013
modified Nov 17 at 08:45pm
created Oct 20, 2015

hayneslab / Chromatin CRISPR Interference

modified Jun 05 at 05:08am
created Aug 04, 2013

hayneslab / Mammalian Basic Parts

Parts used to assemble composites

modified Feb 25 at 06:44pm
created Sep 09, 2014

hayneslab / MicroRNA sensors

Customizable two-state genetic switches, where Red indicates the absence of a microRNA and Cyan indicates the presence of a microRNA. Haynes, K. A., Ceroni, F., Flicker, D., Younger, A., & Silver, P. A. (2012). A sensitive switch for visualizing natural gene silencing in single cells. ACS synthetic biology, 1(3), 99–106. doi:10.1021/sb3000035

modified Nov 17 at 05:41pm
created May 29, 2014

hayneslab / miscellaneous

modified Jul 14 at 07:16pm
created Jan 30, 2015

hayneslab / SB.ASU

Synthetic Biology plasmids distributed through DNASU.

modified Feb 27 at 01:14am
created Feb 26, 2015

hayneslab / Spark Open Research Synthetic Biology

modified Aug 24 at 11:26pm
created Apr 03, 2014

hayneslab / Synthetic Chromatin Actuators

This unique class of transcription factors (gene activators) are fusion proteins that bind to histone marks (H3K27me3) instead of DNA sequences. The first generation of these actuators, called Pc-TFs, was published by Haynes and Silver in 2011. All proteins listed here are tagged with the mCherry red fluorescent protein for easy visualization, tagged with the myc epitope for biochemical analysis, and placed under control of a Tet repressible (dox-inducible) promoter.
Haynes, K. A., & Silver, P. A. (2011). Synthetic reversal of epigenetic silencing. The Journal of biological chemistry, 286(31), 27176–27182. doi:10.1074/jbc.C111.229567

modified Oct 29 at 11:11pm
created Aug 04, 2013

hayneslab / Vectors for Mammalian Expression

DNA plasmid vectors for expression of synthetic genes in mammalian cells.