Parts used to assemble composites
Customizable two-state genetic switches, where Red indicates the absence of a microRNA and Cyan indicates the presence of a microRNA. Haynes, K. A., Ceroni, F., Flicker, D., Younger, A., & Silver, P. A. (2012). A sensitive switch for visualizing natural gene silencing in single cells. ACS synthetic biology, 1(3), 99–106. doi:10.1021/sb3000035
Plasmids submitted to DNASU for the SB.ASU project
This unique class of transcription factors (gene activators) are fusion proteins that bind to histone marks (H3K27me3) instead of DNA sequences. The first generation of these actuators, called Pc-TFs, was published by Haynes and Silver in 2011. All proteins listed here are tagged with the mCherry red fluorescent protein for easy visualization, tagged with the myc epitope for biochemical analysis, and placed under control of a Tet repressible (dox-inducible) promoter.
Haynes, K. A., & Silver, P. A. (2011). Synthetic reversal of epigenetic silencing. The Journal of biological chemistry, 286(31), 27176–27182. doi:10.1074/jbc.C111.229567
DNA plasmid vectors for expression of synthetic genes in mammalian cells.