Welcome to Benchling

Benchling is an intelligent research platform with tools for note-taking, molecular biology, and sample tracking.

Create an account

Email
Already have an account?
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pBR322 (Archived)
  • Sequence Map
    Editing disabled on archived sequence.
    TTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCAAGAGTACAAACTGTCGAATAGTAGCTATTCGAAATTACGCCATCAAATAGTGTCAATTTAACGATTGCGTCAGTCCGTGGCACATACTTTAGATTGTTACGCGAGTAGCAGTAGGAGCCGTGGCAGTGGGACCTACGACATCCGTATCCGAACCAATACGGCCATGACGGCCCGClaIBspDIHindIIItetR CDSP1 ...l)20406080100120140160
    CTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGAGAGAACGCCCTATAGCAGGTAAGGCTGTCGTAGCGGTCAGTGATACCGCACGACGATCGCGATATACGCAACTACGTTAAAGATACGCGTGGGCAAGAGCCTCGTGACAGGCTGGCGAAACCGGCGGCGGGTCAGGACGAGCGAAGCGATGAACCTCGGTGATAGCTGATGCGCTBmtINheIEcoRVFspAItetR CDS180200220240260280300320340
    TCATGGCGACCACACCCGTCCTGTGGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGAGTACCGCTGGTGTGGGCAGGACACCTAGGAGATGCGGCCTGCGTAGCACCGGCCGTAGTGGCCGCGGTGTCCACGCCAACGACCGCGGATATAGCGGCTGTAGTGGCTACCCCTTCTAGCCCGAGCGGTGAAGCCCGAGTACTCGCGAACAAAGCCGCACCCATACCACCGTCCBamHIBanIIBanIISgrAItetR CDS360380400420440460480500520
    CCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGGGGCACCGGCCCCCTGACAACCCGCGGTAGAGGAACGTACGTGGTAAGGAACGCCGCCGCCACGAGTTGCCGGAGTTGGATGATGACCCGACGAAGGATTACGTCCTCAGCGTATTCCCTCTCGCAGCTGGCTACGGGAACTCTCGGAAGTTGGGTCAGTCGAGGAAGGCCACCAccIBtgISalISphIEcoNIHincIItetR CDS540560580600620640660680700
    GCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCGCGCCCCGTACTGATAGCAGCGGCGTGAATACTGACAGAAGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGCGAGACCCAGTAAAAGCCGCTCCTGGCGAAAGCGACCTCGCGCTGCTACTAGCCGGACAGCGAACGCCATAAGCCTTAGAACGTGCGGGAGCGAGTTCPshAItetR CDS720740760780800820840860
    CCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCGGAAGCAGTGACCAGGGCGGTGGTTTGCAAAGCCGCTCTTCGTCCGGTAATAGCGGCCGTACCGCCGGCTGCGCGACCCGATGCAGAACGACCGCAAGCGCTGCGCTCCGACCTACCGGAAGGGGTAATACTAAGAAGAGCGAAGGCCGCCGTAGCCCTACGGGCGCAACGTCCGEagINruIBseYItetR CDS8809009209409609801,0001,0201,040
    CATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCATTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCGTACGACAGGTCCGTCCATCTACTGCTGGTAGTCCCTGTCGAAGTTCCTAGCGAGCGCCGAGAATGGTCGGATTGAAGCTAGTAACCTGGCGACTAGCAGTGCCGCTAAATACGGCGGAGCCGCTCGTGTACCTTGCCCAACCGTACCTAACATCCGCGGCGGGATATGGAACAGBfuAIBspMIBstAPItetR CDS1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATACGGAGGGGCGCAACGCAGCGCCACGTACCTCGGCCCGGTGGAGCTGGACTTACCTTCGGCCGCCGTGGAGCGATTGCCTAAGTGGTGAGGTTCTTAACCTCGGTTAGTTAAGAACGCCTCTTGACACTTACGCGTTTGGTTGGGAACCGTCTTGTATAGGTAGCGCAGGCGGTABsmIStyIPflMIPflMItetR CDS1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGAGGTCGTCGGCGTGCGCCGCGTAGAGCCCGTCGCAACCCAGGACCGGTGCCCACGCGTACTAGCACGAGGACAGCAACTCCTGGGCCGATCCGACCGCCCCAACGGAATGACCAATCGTCTTACTTAGTGGCTATGCGCTCGCTTGCACTTCGCTGACGACGACGTTTTGCAGAAvaIBtgIMscIBsoBIFspAIPpuMIPpuMI1,4201,4401,4601,4801,5001,5201,5401,560
    GCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGACGCTGGACTCGTTGTTGTACTTACCAGAAGCCAAAGGCACAAAGCATTTCAGACCTTTGCGCCTTCAGTCGCGGGACGTGGTAATACAAGGCCTAGACGTAGCGTCCTACGACGACCGATGGGACACCTTGTGGATGTAGACATAATTGCTTCGCGACCGTAACTGGGACTCACTBsgIBsaBIBspEIBpu10I1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAAAAAAGAGACCAGGGCGGCGTAGGTATGGCGGTCAACAAATGGGAGTGTTGCAAGGTCATTGGCCCGTACAAGTAGTAGTCATTGGGCATAGCACTCGTAGGAGAGAGCAAAGTAGCCATAGTAATGGGGGTACTTGTCTTTAGGGGGAATGTGCCTCCGTAGTCACTGGTTTGTro...S1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCCTTTTTTGGCGGGAATTGTACCGGGCGAAATAGTCTTCGGTCTGTAATTGCGAAGACCTCTTTGAGTTGCTCGACCTGCGCCTACTTGTCCGTCTGTAGACACTTAGCGAAGTGCTGGTGCGACTACTCGAAATGGCGTCGACGGAGCGCGCAAAGCCACTACTGCCACTTTTGXmnIPvuIIrop CDS1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGCGTCGGTACTGGGTCAGTGCATCGCTATCGCCTCACATATGACCGAATTGATACGCCGTAGTCTCGTAccIDrdIPfoIBsaAIBsmBIEsp3IPflFIBstZ17ITth111I2,1202,1402,1602,1802,2002,2202,2402,260
    GATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAACTAACATGACTCTCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTEarINdeISapITatIBspQIBsrBIBstAPI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCDrdIPciIAflIIIRNAII transcriptpMB1 ori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGBciVIBseYIRNAII transcriptpMB1 ori2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCAlwNIRNAII transcriptpMB1 ori2,8202,8402,8602,8802,9002,9202,9402,960
    ACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAAcuIpMB1 oriRNAII transcriptRNAI Transcript2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGbla CDS3,1603,1803,2003,2203,2403,2603,2803,3003,320
    AGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGATCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTAhdIBsaIBsrDIbla CDS3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAATCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGACGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTAseIPstIBsrDIbla CDS3,5203,5403,5603,5803,6003,6203,6403,660
    GGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGBtsIBtsIPvuIScaITatIBtsαIBtsαIbla CDS3,6803,7003,7203,7403,7603,7803,8003,8203,840
    AACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAATTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTGTGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTXmnIHincIIbla CDS3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAcuIEarISspIbla CDSperiplasm signal4,0404,0604,0804,1004,1204,1404,1604,1804,200
    TCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAAAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGAAGTTCTTApoIZraIAatIIBciVIBsrBIEcoRI4,2204,2404,2604,2804,3004,3204,3404,360
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on archived sequence.
    4361 bp32503500375040002505007501000tetR CDSbla CDSperiplasm signal

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    4361