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Notebook entry
Notebook entry
Tuesday, 12/15/2015
(Deleted)
The DNA sequence corresponding to this note no longer exists.
Figure 1. DsRed2 cloning into pDusk vector (12/15/2015)
Materials
Table1
A A | B B | C C | D D | E E | |
1 1 | Number of Reactions | 80 | 40 | 20 | |
2 2 | Component | Amount (uL/tube) | Master Mix (uL) | Master Mix (uL) | Master Mix (uL) |
3 3 | Water | 16 | 1280 | 640 | 320 |
4 4 | 10x Buffer | 2 | 160 | 80 | 40 |
5 5 | Enzyme | 1 | 80 | 40 | 20 |
6 6 | Master Mix Total | 19 | 1520 | 760 | 380 |
7 7 | Template DNA | 1 | |||
8 8 | Reaction Total | 20 |
Wednesday, 12/16/2015
Combine above reaction in tube
Incubate at 37ยฐC for an hour
Run gel
Extract band and save
Reference plasmid map pDusk-DsRed2 tomorrow.
Thursday, 12/17/2015
Drag and drop an image in to view it inline and take notes about it:
pAS329_ligation_check.png
pAS329_ligation_check.png
Lane 1: DNA ladder
Lane 2: undigested
Lane 3: digested vector
Lane 4: insert DNA
Lane 5: ligation tube
Friday, 12/18/2015
Link to other Benchling files by dragging them in from the file browser at the left.
pBR322
pBR322
pBR322 is the backbone I'm going to use for this experiment.
The ampR gene is penicillin beta-lactamase.
It also has the tetR gene and the rop gene.
Saturday, 12/19/2015
1.
Add nuclease
Benchling_Marketing_1-pager.pptx
Benchling_Marketing_1-pager.pptx
Friday, 8/12/2016