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pACD3-EcI5G
  • Sequence Map
    Editing disabled on read-only sequence.
    gaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccatcttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaEcoRIPvuIICm(R)20406080100120140160
    tgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacggctagttgcagagtaaaagcggttttcaaccgggPasIBsaAIBsmBIEsp3IPflMIBpu10ICm(R)180200220240260280300320340
    agggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtatttattcggcgcaaagtgcgtcgggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtcccttcccgaagggccatagttgtccctgtggtcctaaataaataagacgcttcactagaaggcagtgtccataaataagccgcgtttcacgcagcccactacgacggttgaatgactaaatcacatactaccacaaaaactccacgaggtcaccgaagacaaagatagtcgacagggaPvuII360380400420440460480500520
    cctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagcgctagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggaggacaagtcgatgactgccccaccacgcattgccgttttcgtggcggcctgtagtcgcgatcgcctcacatatgaccgaatgatacaaccgtgactactcccacagtcacttcacgaagtacaccgtcctcttttttccgacgtggccacgcagtcgtcttatacactatgtcctBmtINheIXmnIp15a540560580600620640660680700
    tatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagatataaggcgaaggagcgagtgactgagcgatgcgagccagcaagctgacgccgctcgcctttaccgaatgcttgccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgaggcggggggactgttcPfoISacIIp15a720740760780800820840860
    catcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagtgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtgcggactgtgagtca8809009209409609801,0001,0201,040
    tccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagtacg1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatccggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttctagBstBI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atcttattaatcagataaaatatttctagctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgtaattctcatgtttgacagcttatcatcgataagctcaaggagatggcgcccaacagtcccccggccacggggcctgcctagaataattagtctattttataaagatcgatctaaagtcacgttaaatagagaagtttacatcgtggacttcagtcggggtatgctatattcaacattaagagtacaaactgtcgaatagtagctattcgagttcctctaccgcgggttgtcagggggccggtgccccggacggEarISspIEcoO109I1,4201,4401,4601,4801,5001,5201,5401,560
    accatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgacttggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagagctagggcgctttaattatgctgaNaeIBglIINgoMIVT7...r1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cactataggggaattgtgagcggataacaattcccctctagaagaattcccatgccaaactggtgctcgaatgtgcgacatgaagtcgcctgaataattgttccagcggagttcgattccgtcagggaacctgatgttccgtcatcagtagcctactgacacatgcgtcactggtgtgatatccccttaacactcgcctattgttaaggggagatcttcttaagggtacggtttgaccacgagcttacacgctgtacttcagcggacttattaacaaggtcgcctcaagctaaggcagtcccttggactacaaggcagtagtcatcggatgactgtgtacgcagtgaccaAhdIXbaIEcoRIEcI5-IBS templateEcI5 TargeTronIBS1/2S primerEcI5-IBS-F primerlac operator1,7601,7801,8001,8201,8401,8601,8801,9001,920
    aacggtggggtgtgaagctgtcaggacaatgaaaccggatcttcggatcgcatgaaaccgtgaggttacatgtaatctgccagcatcaggcggaggaggtctaggctcggtagcatgactaacatatgtgaactgctgaagcgtcgttaaccagaacaagccaaagatgctgacattgccaccccacacttcgacagtcctgttactttggcctagaagcctagcgtactttggcactccaatgtacattagacggtcgtagtccgcctcctccagatccgagccatcgtactgattgtatacacttgacgacttcgcagcaattggtcttgttcggtttctacgactgtNdeIEcI5 TargeTronEcI5-IBS templateEBS2AS primerEcI5-IBS-R primer1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggcttgaaccaaaaggtatgtggttggttactcctctgtttgctaggggtacacggacaataaaccaccggtgttgtgagcagagtctaacctactgttgttattcaggtggaacatggtaagcccgtatcgctgcctcgtgaggcaggttaaccgcaagggatactgttggcggccgaacttggttttccatacaccaaccaatgaggagacaaacgatccccatgtgcctgttatttggtggccacaacactcgtctcagattggatgacaacaataagtccaccttgtaccattcgggcatagcgacggagcactccgtccaattggcgttccctatgacaaccgccXcmICspCIEcI5 TargeTronEcI5-EBS templateEBS1S primerEcI5-EBS-G-F primerEBS2AS primer2,1202,1402,1602,1802,2002,2202,2402,260
    tgcgggtaaaagaaggtggaaaaagcgaatgccagtctgtaatggaccggataagggttgagcccggcaacattaccctacgcgaaagcgggcagacttccactgggtctttcattacgagagagtttgagtaatcttccagaaaggaaaagcagatgactgagcaggctacaacacgcccattttcttccacctttttcgcttacggtcagacattacctggcctattcccaactcgggccgttgtaatgggatgcgctttcgcccgtctgaaggtgacccagaaagtaatgctctctcaaactcattagaaggtctttccttttcgtctactgactcgtccgatgttgAvaIIEcI5 TargeTronEcI5-EBS templateEcI5-EBS-R primer2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctgtaaaggtgcgtccttacttaacggtgactcctggcacagtatcaactggcgtcagtgctatcgggaagtgcggagactgcaagcgcgtatcgtaaaggcaaacgcgtcacttgttcatgcgaaaggtatacatgtagtgaaaccggctcatgagagtgggcttagaaaggctgacatttccacgcaggaatgaattgccactgaggaccgtgtcatagttgaccgcagtcacgatagcccttcacgcctctgacgttcgcgcatagcatttccgtttgcgcagtgaacaagtacgctttccatatgtacatcactttggccgagtactctcacccgaatctttccgaMluIEcI5 TargeTron2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgagccgtatgccgggaaactggcacgtacggttcttagggggcggtgatgcagtaatgcatcactgctacccgatcgtatgtatttttctctgcagccccgtttaaacccctagtacccccgtttaaaccctaagaaggagatatacctatgactgagcaggctacaacctgtaactcggcatacggccctttgaccgtgcatgccaagaatcccccgccactacgtcattacgtagtgacgatgggctagcatacataaaaagagacgtcggggcaaatttggggatcatgggggcaaatttgggattcttcctctatatggatactgactcgtccgatgttggacatBarINsiIPstIPvuIBsaAIBsiWIEcI5 TargeTronIEP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    aaggtgcgtccttacttaacggtgactcctggcacagtatcaactggcgtcagtgctatcgggaagtgcggagactgcaagcgcgtatcgtaaaggcaacacgagaaggtaaacatggcaaggtcaaatccttgcaatggatactgactcactcgtttagtggcagagcagttgcttccacgcaggaatgaattgccactgaggaccgtgtcatagttgaccgcagtcacgatagcccttcacgcctctgacgttcgcgcatagcatttccgttgtgctcttccatttgtaccgttccagtttaggaacgttacctatgactgagtgagcaaatcaccgtctcgtcaacgIEP2,8202,8402,8602,8802,9002,9202,9402,960
    tgtcagacgagttacagagaatagcgggaaacgtactcctggggttgatggtcagacatggtcttccccggaggttaaatttcttgctattaacctgctgaaacggcgtggttacaaaccacagccacttaagcgggtttatatacctaagtcaaatgggaagtctcgtccccttacagtctgctcaatgtctcttatcgccctttgcatgaggaccccaactaccagtctgtaccagaaggggcctccaatttaaagaacgataattggacgactttgccgcaccaatgtttggtgtcggtgaattcgcccaaatatatggattcagtttacccttcagagcaggggaaBbsIAflIIPflFITth111IIEP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gggatacccactatgaaggatcgggcgatgcaggcgttgtatttgctggctcttgagcccgttgctgaagtcactgctgaccagagatccttcggctttcggactggaaggtctactgcagatgcgatagcacagtgcttctgtgttcttgcgcagaaaacatccgctgaatgggccctatgggtgatacttcctagcccgctacgtccgcaacataaacgaccgagaactcgggcaacgacttcagtgacgactggtctctaggaagccgaaagcctgaccttccagatgacgtctacgctatcgtgtcacgaagacacaagaacgcgtcttttgtaggcgacttacccFspIPstIIEP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tgcttgagggggatatacggggatgctttgacaatatatctcatcaatggctaatcgataacacctcaacagacagacaaatcctgacaaagtggcttaaagcgggttacagggaaaaggggcagttattccctgtaaattcaggaactcctcaggggggaataatttctcctgtacgaactccccctatatgcccctacgaaactgttatatagagtagttaccgattagctattgtggagttgtctgtctgtttaggactgtttcaccgaatttcgcccaatgtcccttttccccgtcaataagggacatttaagtccttgaggagtccccccttattaaagaggacaXmnIBsu36IIEP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    actggctaacattgctctggatggactggaggcgcttcttgcttcggagtttaaaaagagaacagtcaagggacgattggttaacccgaaagttaactatgtgagatatgctgatgattttattatcactggagagtcaaaagaacttcttgagtctcaggtattacccgtcgtctgaccgattgtaacgagacctacctgacctccgcgaagaacgaagcctcaaatttttctcttgtcagttccctgctaaccaattgggctttcaattgatacactctatacgactactaaaataatagtgacctctcagttttcttgaagaactcagagtccataatgggcagcagIEP3,5203,5403,5603,5803,6003,6203,6403,660
    aggcggtttatggctgaaagaggattaatgctttctccggaaaagacgaaaatcacacatatcgaggaaggatttgattttcttggacagaacattcgcaaatatggcggtaagatgctgatcaaaccctctaaagccaatgtcagttcctttttaaagaaaatacgcgctgtaatccgccaaataccgactttctcctaattacgaaagaggccttttctgcttttagtgtgtatagctccttcctaaactaaaagaacctgtcttgtaagcgtttataccgccattctacgactagtttgggagatttcggttacagtcaaggaaaaatttcttttatgcgcgacattIEP3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tcaaaggcaacaaagctatggatcagcttacgttgatccgcatgctgaacccaatgatcaaagggtgggcggcttaccatcaacacattgtggccaaggtcgcatttaacaaagttgacaatgagatctggctggctttatggcgttgggctgttcggcgtcatcccaataagggagtttccgttgtttcgatacctagtcgaatgcaactaggcgtacgacttgggttactagtttcccacccgccgaatggtagttgtgtaacaccggttccagcgtaaattgtttcaactgttactctagaccgaccgaaataccgcaacccgacaagccgcagtagggttattcccMscISphIBglIIDraIIIIEP3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aaagaagtggattcggaaaagatatttccatcaacaaggtgcccgaaactggtctttctccacggctaccggagaactccttgccaacggtaagcctcagtatgcaaatctgaggaaagcaatcgatacgcctatcaaccggtttaaaccgattaaaattgcagctaatccgttctttcttcacctaagccttttctataaaggtagttgttccacgggctttgaccagaaagaggtgccgatggcctcttgaggaacggttgccattcggagtcatacgtttagactcctttcgttagctatgcggatagttggccaaatttggctaattttaacgtcgattaggcaagBaeGIIEP4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gatcctcaatgggagatgtactttgaggagcgttgtgcagacaaaatgcggcacaaactgcaaggacggaaaaagctcattcaaatctggtttgagcagcggggccgatgccccatatgtgatgagaggattacatctgattcccagtggcaggttcatcacatcatacggcgagctaggagttaccctctacatgaaactcctcgcaacacgtctgttttacgccgtgtttgacgttcctgcctttttcgagtaagtttagaccaaactcgtcgccccggctacggggtatacactactctcctaatgtagactaagggtcaccgtccaagtagtgtagtatgccgctcNdeIBstAPIIEP4,2204,2404,2604,2804,3004,3204,3404,360
    tcgatggagggagcaactgcctctccaatttgattatgctgcatccgatgtgtcacacacttgttcatgcgaaaggtatacatgtagtgaaaccggctcatgagagtgggcttagaaaggcttgactagtcggtgttctcgaggttgggtaccgaaatatatctcgagcacccgtagctacctccctcgttgacggagaggttaaactaatacgacgtaggctacacagtgtgtgaacaagtacgctttccatatgtacatcactttggccgagtactctcacccgaatctttccgaactgatcagccacaagagctccaacccatggctttatatagagctcgtgggcaAvaIAvaIKpnISpeITliITliIXhoIXhoIBsoBIBsoBIAcc65IPaeR7IPaeR7IIEP4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcgccaagctcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaatagagcctcgtgacaggctggcgaaaccggcggcgggtcaggacgagcgaagcgatgaacctcggtgatagctgatgcgctagtaccgctggtgtgggcaggacacctagcggttcgagcggctaccatcacaccccagaggggtacgctctcatcccttgacggtccgtagtttaBsaI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgccaggcatcaaattaagcagttttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagaggactcatcctgtttaggcggccctcgcctaaacttgcaacgcttcgttgccgggcctcccaccgcccgtcctgcgggcggtatttgacggtccgtagtttaattcgtcAclIT14,7404,7604,7804,8004,8204,8404,8604,8804,900
    aaggccatcctgacggatggcctttttgcgtttctacaaactcttcctgtcgtcatatctacaagccatccccccacagatacggtaaactagcctcgtttttgcatcaggaaagcagaacgccatgagcggcctcatttcttattctgagttacaacagtccgcaccgctgtccttccggtaggactgcctaccggaaaaacgcaaagatgtttgagaaggacagcagtatagatgttcggtaggggggtgtctatgccatttgatcggagcaaaaacgtagtcctttcgtcttgcggtactcgccggagtaaagaataagactcaatgttgtcaggcgtggcgacaggEarIT24,9204,9404,9604,9805,0005,0205,0405,060
    ggtagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgccctgcaccattatgttccggatctccatcgaggaaggccacccgcgccccgtactgatagcagcggcgtgaatactgacagaagaaatagtacgttgagcatcctgtccacggccgtcgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgggacgtggtaatacaaggcctagaBbsIBcgINaeIPshAINgoMIVEcoO109I5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gcatcgcaggatgctgctggctaccctgtggaacacctacatctgtattaacgaagcgctaaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacacgtagcgtcctacgacgaccgatgggacaccttgtggatgtagacataattgcttcgcgattggcaaaaatagtccgagaccctccgtcttatttactagtatagcagttaataatggaggtgcccctctcggactcgtttgaccggagtccgtaaactcttcgtgtgccagtgtBpu10IBsu36I5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaatttgctttcgaatttctgccattcatccgcttattatcacttattcaggcgtagcaccaggcgtttaagggcaccaataactgcctgacgaaggccatcagttatttggccatttggtcgttatctgtattcgccgataaattgctgggacgggacttggctgctggcccagcttaaacgaaagcttaaagacggtaagtaggcgaataatagtgaataagtccgcatcgtggtccgcaaattcccgtggttattgacggaBaeGIBstBIPflFITth111I5,4405,4605,4805,5005,5205,5405,5605,5805,600
    taaaaaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagtatttttttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtctgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaNcoIScaISspICm(R)5,6205,6405,6605,6805,7005,7205,7405,760
    tgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaMscIPasIBsmBIEsp3IPflMICm(R)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgcAclICm(R)5,9605,9806,0006,0206,040
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    6049 bp45005000550050010001500T1T2p15aCm(R)

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    6049